báo cáo khoa học: "Engineering of the E coli Outer Membrane Protein FhuA to overcome the Hydrophobic Mismatch in Thick Polymeric Membranes" pdf
báo cáo khoa học: " Engineering of an E. coli outer membrane protein FhuA with increased channel diameter" doc
... we report the increasing of the channel diameter by genetic engineering. Results: The FhuA Δ1-159 diameter increase has been obtained by doubling the amino acid sequence of the first two N-terminal ... the number. Therefore the good correlation between channel sur- face area in crease and kinetic results can be either due to an increase in channel diameter or to an...
Ngày tải lên: 11/08/2014, 00:23
báo cáo khoa học: "Engineering of the E. coli Outer Membrane Protein FhuA to overcome the Hydrophobic Mismatch in Thick Polymeric Membranes" pdf
... address the question whether the FhuA Δ1-159 Ext really acts as a channel or whether the observed kinetics are due to the locally perturbed polymer membrane by the presence of the protein, the ... limit to ensure the insertion of the FhuA Δ1-159 Ext into the E. coli membrane. An increased hydrophobic mismatch between the protein and the lipid...
Ngày tải lên: 11/08/2014, 00:23
báo cáo khoa học:" Preferences of diabetes patients and physicians: A feasibility study to identify the key indicators for appraisal of health care values" pot
... behavioral functioning These were measured using the adapted version of the Diabetes Health Profiles DHP-1 questionnaire for people with type 2 diabetes, the DHP-18 [17]. The 18 items were summed into three subscale ... 3.95% and the yearly increase of the preva- lence is at 5.7%. Six percent of mortality is related to diabetes and 5% of the resources of the health...
Ngày tải lên: 12/08/2014, 01:21
Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx
... Protein levels of KLF4 were determined by western blot. (C) mRNA levels of hSR-BI were determined by real-time PCR. (D) Protein levels of hSR-BI were determined by western blot. The relative values ... oxLDL-stimulated cells, but further increased the expression of SR-BI in response to HDL 3 stimulation compared with the vector control group. In order to observe the...
Ngày tải lên: 18/02/2014, 04:20
Báo cáo khoa học: Engineering of a monomeric and low-glycosylated form of human butyrylcholinesterase pdf
... sites yielded poor expression levels as well (Table 2, seven and nine sites off) due to retention of the protein inside the cell, as shown by Western blotting. As the expression level of these ... under- standing of some aspects of the difference in speci®city between AChE and B ChE, they are not satisfactory for enzyme engineering. The crystal structure of human BCh...
Ngày tải lên: 17/03/2014, 11:20
Báo cáo khoa học: Comparison of human RNase 3 and RNase 7 bactericidal action at the Gram-negative and Gram-positive bacterial cell wall pot
... depolarizing cell membranes, as observed in RNase 3, when E. coli cells are pretreated with EDTA, thus suggesting that the main differences may be restricted to the bacterial outer barrier. These results ... and the time required to achieve half of total membrane depolarization was esti- mated from the nonlinear regression curve. E. coli cells were also incubated in...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo khoa học: Inhibition of a-synuclein fibrillization by dopamine analogs via reaction with the amino groups of a-synuclein Implication for dopaminergic neurodegeneration pot
... that link to the aromatic ring of quinone and the side chains of the protein. The NMR experi- ments provide convincing evidence in support of the reaction between amino groups of Lys residues and ... h. There is no significant change of the peaks in the amide region except for three extra peaks (arrows indicated) emerging in each spectrum. The peaks appearing...
Ngày tải lên: 23/03/2014, 15:20
Báo cáo khoa học: Saporin and ricin A chain follow different intracellular routes to enter the cytosol of intoxicated cells pptx
... their C-termini. In the ER, the presence of a higher pH allows detachment of the retrieved protein from the KDEL receptors [4]. The REDLK Keywords anticancer therapy; bacterial toxins; intracellular ... KDEL [29]. The PEA C-terminal sequence binds to KDEL recep- tors [2] and overexpression of this receptor makes cells more susceptible to the PEA [3]. The terminal...
Ngày tải lên: 23/03/2014, 15:21
Báo cáo khoa học: Engineering of monomeric FK506-binding protein 22 with peptidyl prolyl cis-trans isomerase Importance of a V-shaped dimeric structure for binding to protein substrate docx
... close proximity to the Met8 of the other monomer. Both residues are located close to the bottom of the cleft of the V-shaped structure. Therefore, it is strongly expected that the mutant protein, termed NNC- FKBP22, ... subfamily proteins and is required for PPIase activity. In addition, six of the seven Tyr residues are located in the C-domain. Therefore, th...
Ngày tải lên: 30/03/2014, 01:20
Báo cáo khoa học: Detection of nucleolar organizer and mitochondrial DNA insertion regions based on the isochore map of Arabidopsis thaliana ppt
... indicates a decrease of the GC content or an increase of the AT con- tent, otherwise, a drop in the curve, i .e. k¢ < 0 indicates an increase of the GC content or a decrease of the AT content. Acknowledgements We ... content, while a drop in the curve indicates an increase of the GC content. The slope of the curve denotes the vari- ation rate of the...
Ngày tải lên: 30/03/2014, 20:20