báo cáo khoa học: " Antimicrobial activity of spherical silver nanoparticles prepared using a biocompatible macromolecular capping agent: evidence for induction of a greatly prolonged bacterial lag phase" potx

... biocompatible macromolecular capping agent: evidence for induction of a greatly prolonged bacterial lag phase. Journal of Nanobiotechnology 2010 8:34. Submit your next manuscript to BioMed Central and take ... than S. aureus to the keratin-based Ag Nps. Conclusions In this work we have evaluated the antimicrobial prop- erties of a biocompatible macromolecu...

Ngày tải lên: 11/08/2014, 00:22

... vertebrates r anging from fish to humans. Histone antimicrobial activity was first demonstrated in 1958 for histones A and B purified from calf thymus, which exhibited antibacterial activity against various ... hyaline, semigranular, and granular cells. The hyaline cells are associated with coagulation a nd phagocytosis while the semigranular and granular cells are involved in phagoc...

Ngày tải lên: 07/03/2014, 16:20

... substrates have been identified. Although calpain 7 contains a C2-like domain, it lacks a penta-EF-hand domain and is classified as an atypical calpain. As one of the significant structural features, mammalian ... Germany). Construction of plasmids Cloning of human calpain 7 cDNA and construction of mammalian expression plasmids for various mGFP-fused calpain 7 mutants (FLAG–CHMP1B...

Ngày tải lên: 18/02/2014, 04:20

... 253–257. 34 Takeshima K, Chikushi A, Lee KK, Yonehara S & Matsuzaki K (2003) Translocation of analogues of the antimicrobial peptides magainin and buforin across human cell membranes. J Biol ... buforins and pos- sibly HN. The inflammation is also accompanied by the release of large amounts of ATP from stimulated cells [13,25]. A large body of evidence indicates that catio...

Ngày tải lên: 18/02/2014, 14:20

... 4B). Presence of Xantha-g mRNA in xantha-h mutants A possible explanation for the absence of 70 kDa XAN-G protein in the xantha-h mutants could be that the XAN-H protein affects the level of Xantha-g mRNA. ... primers EXgLp67 (5¢-CGTAGATACAAACTTGTTCTCGGT AT-3¢) and EXgUp70 (5¢-GCATTTATTCCCTTCCGTG GAGACT-3¢) are separated by two introns in the chromo- somal Xantha-g DNA. Therefore a...

Ngày tải lên: 19/02/2014, 12:20

... expression for a single pK a (see the legend to Table 1 for details). The values for k cat and bk cat ,ateachpH,weretakenfrom fitting of k app vs. BTC concentration data for each mutant (data not shown). 318 ... k cat activity was effectively zero, and bk cat activity was approaching zero (Fig. 3). The titration of k cat was monophasic, with a pK a of 6.7 ± 0.09. Howe...

Ngày tải lên: 20/02/2014, 23:20

... TaHsp17.8C-II contains either fewer subunits than TaHsp16.9C-I, or has a more nonglobular shape. Finite element analysis of the data [28] estimates a molecular mass of 201 kDa for TaHsp16.9C-I and 173 kDa for ... University of Arizona Experiment Station Funds, and American Cancer Society Faculty Research Award #FRA-420 to E. V. G. J. L. was a recipient of a National Instit...

Ngày tải lên: 07/03/2014, 15:20

... multiples of AKKARA or ARKKAAKA [15,20] were synthesized and tested in bactericidal assays using E. faecalis as the test organism, and the results demonstrate that these peptides are also antibacterial. ... visualized as a clear zone around each well after 18–24 h of incubation at 37 °C for bacteria and 28 °CforC. albicans.ForE. coli the dose– response characteristics of the RDA w...

Ngày tải lên: 07/03/2014, 15:20

... 477–487. 15. Chopade, B .A. , Shankar, S., Sundin, G.W., Mukhopadhyay, S. & Chakrabarty, A. M. (1997) Characterization of membrane–asso- ciated Pseudomonas aeruginosa Ras-Like proteins Pra, a GTP binding ... CAC GGA TCC GTG ACC GAA-3¢, carrying BamHI at its 5¢-end and a reverse primer 5¢-TCC GGA TGA GCA TTC ATC AGG-3¢. The internal primers were 5¢-GCC AGC CAG CAA TAC GCC GAA-3¢ and...

Ngày tải lên: 08/03/2014, 08:20

... chromatography and analysis by MS (MALDI and, separately, LC ⁄ MS) identified AK as a contaminant that could cause the apparent ADP hydrolysis activity when coupled with the ATPase activity of NtrC1 ... (S-frac- tion, 30 537.5 ± 6 Da; Q-fraction, 30 537.0 ± 6 Da). However, the S-fraction lost apparent ADPase activity and the Q-fraction had an elevated apparent ADPase activity. [Note...

Ngày tải lên: 16/03/2014, 04:20