báo cáo khoa học: "Nanofibers and nanoparticles from the insectcapturing adhesive of the Sundew (Drosera) for cell attachment" ppsx

... 147 cells/mm2 for the negative control, 1681 cells/mm2 for the positive control, and 1253 cells/mm2 for the Sundew adhesive coated surface. Due to the non-uniformity i n the coating of the Sundew ... nanofiber and nanoparticle basednanocomposite from the adhesive of Sundew can be used for tissue engineering, and opens the possibility for further examination of natural plant adhesives for ... substrate to promote the attachment of PC12 neu-ron-like cells, and may be used for a variety of other cell types. Further study into the role of the nanoparticles within the nanocomposite will...

Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx

... trafﬁc and ⁄ or degradation of the IR. How-ever, despite a recent report showing the involvement of Grb10 in the ubiquitination and proteasomal degra-dation of the IR in transfected cells [37], there ... inhibits the tyrosine kinase activity of the IR [7]. On the basis of the crystal structure of the tyrosine kinase domain of the IR in complex with the PIR ⁄ BPS domain of Grb14,Depetris et al. [8] have ... interesting newinformation on the role of this adaptor protein. The endosomal colocalization and ⁄ or cointernaliza-tion of the activated IR and Grb14 raises the questionas to whether Grb14 could,...

Tài liệu Báo cáo khoa học: Temperature and phosphate effects on allosteric phenomena of phosphofructokinase from a hibernating ground squirrel (Spermophilus lateralis) pptx

... changes in the extent to which the binding of allosteric ligandmodiﬁes the afﬁnity of enzyme for substrate. The Hanes–Wolf plot in Fig. 3(B) conclusively demon-strates that the Kmvalue for Mg.ATP ... heart and leg muscle [12]. In the case of leg muscle, the con-centration of the potent activator, F2,6P2, decreased from a level ﬁve times the Kavalue in euthermic ani-mals to one-half the ... (Ea) values for the 3–15 °C tempera-ture range of the plots were 103.2 ± 0.2 and 80.0 ± 1.2 kJ mol)1in the absence and presence of phosphate. For the 15–45 °C temperature range, the Ea values...

báo cáo khoa học: "Naturally occurring nanoparticles from English ivy: an alternative to metal-based nanoparticles for UV protection" doc

... adhesive effect of these nanoparticles will allow the ivy nanoparticles to remain on the skin for a longerperiod of time, and thus enhance their UV protectiveeffect. The combination of these unique ... penetratethrough the SC layer [44]. Therefore, to understand ivynanoparticle diffusion and penetration in human skin, itis essential to understand the diffusion process of these nanoparticles in the SC.There ... effects using a standard spectrophotometric assay. Next the cell toxicity of the ivy nanoparticles was compared to TiO2 nanoparticles using HeLa cells. The biodegradability of these nanoparticles...

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... Determination of Vmax and Km for DAT, NET and SERT in the presence and absence of p25a. HEK-293-MSR cells were tran-siently transfected with DAT, NET or SERT and with p25a or mockvector. The cells ... in the central core and were similar to those required for vesicle binding. Co-expression of p25a and the dopamine transporter in HEK-293-MSR cells sensitized them to the toxicity of extracellular ... vesicles. Because the vesicle-binding proﬁle of biotinylated p25a differed from that of the endoge-nous p25a, the ﬁlter used for biotinylated p25a wasprobed for the ER marker BIP and the synaptic...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf

... neurodegenerative disease.Synthesis and secretion of GIF in the brainCellular and temporal localization of GIFsynthesisWhilst the MT1 and MT2 isoforms are ubiquitouslysynthesized throughout most ... MT isoform has different and distinct functions within the brain. Furthermore, the discovery and continued investigation of this brain-spe-ciﬁc MT isoform has led to intense interest in the roles of ... of GIF within the brain. Further studies are required todetermine the function of GIF within the normal,injured and neurodegenerative brain, and to establish the therapeutic potential of the protein.AcknowledgementsThis...

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... almostlinear and can be obtained by calculating the slope of the curves of Pyr-ONC (M23L) vs. time. From the rates of cyclizationshowninTable1,itcanbeinferredthattheformation of the N-terminal ... analyzed by MALDI-TOF MS. The addition of the protease is considered the zero time point. The relative intensities of the peaks from the mass spectrawere used to calculate the fraction of Pyr-ONC (M23L), ... onconase and the variant. We are also indebted toDr Francesc Canals for his help in the mass spectra acquisition and for the discussion of the results. This work was supported by grants from the...

Báo cáo khoa học: Adenylyl cyclase Rv0386 from Mycobacterium tuberculosis H37Rv uses a novel mode for substrate selection ppt

... [31]. The substrate speciﬁcity of Rv0386 may be deter-mined by the concentrations of available ATP and GTP at the cellular location of the enzyme, yet the intracellular concentrations of ATP and ... analogy to the Rv0386(1)175)N106D mutant wealso generated Rv0386(1)175)Q57E. On the one hand the mutation eliminated the hydrogen-bond donorproperty of the resident Q57; on the other hand a ... predicted for any of these proteins. We areaware, of course, that this does not exclude the exist-ence of novel, hitherto unknown cGMP binding pro-teins in the pathogen.In conclusion the characterization...

Báo cáo khoa học: Apoptosis and autophagy: BIM as a mediator of tumour cell death in response to oncogene-targeted therapeutics pptx

... as a monotherapy [38] highlight the need for a greater understanding of how these compounds actto initiate cell death, which could guide the selection of suitable therapeutic partners for combination ... (PKCs). Treatment of multiple myeloma cells with UCN-01 alone resulted in the activation of ERK1 ⁄ 2 and in the phosphorylation and loss of BIMEL; however, co-administration of the MEK1 ⁄ 2 inhibitor ... cells exhibit aseries of hallmarks that set them apart from normalcells [31] and driver mutations are thought to promote the acquisition and underpin the maintenance of thesetumour-speciﬁc traits....

Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf

... structures i s the lack of a subdomain formedby two b sheets and one a helix connecting the bsheet 2 of the b subunit, which carries the catalytic glutamate, with the inner l ayer of a helices ... 553 Da and a n isoelectric point of 4.9 1 waspredicted for the encoded p rotein. B oth values w ere inagreement with the observed molecular mass and the elution of the enzyme during the ®nal ... induction of the protein, E. coli stopped growing. T hese observationsmay point to a severe impairment of the cellular metabolism of E. coli by the C. prop ionicum enzyme. Although the reason for...

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