... other l-aminoacids, including l-aspartate, l-glutamine, l-alanine,l-arginine, l-cysteine, l-proline, l-phenylalanine,l-lysine, l-tryptophan, l-isoleucine, l-tyrosine, l-histi-dine, l-leucine, ... standard conditions [32] following the instructions of the manufacturer.Cloning and sequencing of the alcoholdehydrogenase encoding gene The identification of the gene encoding an alcohol dehy-drogenase ... (underlined in the sequences). In order tointroduce an NcoI restriction site, an extra alanine codon(GCA) was introduced in the tdh gene by the forwardprimer BG 1279 (bold in the sequence). The...