báo cáo khoa học: "Stability and assembly in vitro of bacteriophage PP7 virus-like particles" pptx

... quantity of RNA Assembly of PP7 VLPs in vitro in the presence of MS2 opera-tor RNAFigure 9 Assembly of PP7 VLPs in vitro in the presence of MS2 opera-tor RNA. All reactions contained 0.1 nmol PP7 ... proximity within the dimer of the N-terminus of onesubunit to the C-terminus of the other suggested a simplemeans of introducing an inter-dimer covalent bond.Duplicating the coat gene and joining the ... proteindimer. Protein from the peak fractions was used in the in vitro assembly reactions. In vitro VLP assembly Purified dimeric PP7 coat protein (0.1 nmol) was addedto reactions containing...

Báo cáo khoa học: Stability and ﬁbril formation properties of human and ﬁsh transthyretin, and of the Escherichia coli transthyretin-related protein potx

... to the retinol-binding protein when it is loaded with retinol [1]. TTRis mainly expressed in the adult liver, the choroidplexus of the brain, and the retina [2,3]. TTR isinvolved in three ... SwedenTransthyretin (TTR) is a homotetrameric plasma pro-tein that binds and transports the thyroid hormones3,5,3¢-triiodo-l-thyronine and 3,5,3¢,5¢-tetraiodo-l-thyr-onine (thyroxine) and retinol by binding ... stability of pro-teins [38–40]. These include tighter hydrophobic packing of the protein core [41–43], increased electrostatic inter-actions on the surface of the protein [44,45], more pro-lines and...

Báo cáo khoa học: Substrate and inhibitor speciﬁcity of Mycobacterium avium dihydrofolate reductase pptx

... of aspartate 27 of dihydrofolate reductasefrom Escherichia coli in interconversion of active and inactive enzyme conformers and binding of NADPH.J Biol Chem 265, 5579–5584.19 Birdsall B, Andrews ... importance of D31 and L32as substrate-binding, cofactor-binding and inhibitor-binding sites.The objectives of this study were to: (a) utilize site-directed mutagenesis to modify Asp31 and Leu32 ... enzyme in the folate biosyn-thetic pathway. Information regarding key residues in the dihydrofolate-binding site of Mycobacterium avium dihydrofolate reductase is lacking. Onthe basis of previous...

Tài liệu Báo cáo khoa học: Applications and trends in systems biology in biochemistry docx

... decreases of dopamine transporter in combination with monoamineoxidase inhibition appear to have thepotential of efﬁciently increasingextracellular dopamine levels at thesame time as minimizing ... protein kinase; MCF, macrophare chemotactic factor; MCIP, modulatory calcineurin-interact-ing protein; MDCK, Madin Darby canine kidney; MEF, mouse embryonic ﬁbroblast; MEK, MAP kinase/ERK kinase; ... acid,argininecatabolismto NO and polyaminesNG, aortaendothelialcellsLow afﬁnity transporter and arginaseshare the control of the ﬂuxesthrough the pathways involvingarginine as a precurser of nitricoxide and polyaminesODE...

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... (G) Sinus gland axonal arborizationscontainingD-Trp4 VIH (green) and D-Phe3 CHH (red). (H) Enlargement of axon terminals in the sinus gland with labelling as in (G).Peptidyl isomerization in ... (made in rat) and gp-anti-dW4 (produced in guineapig), two antisera discriminating CHH isomers (gp-anti-pQlmade in guinea pig and rb-anti- pQd in rabbit) [38] and anantiserum raised against ... chirality of a single aminoacyl resi-due within a peptide chain is a subtle and intriguingmechanism that remains poorly known to date, and which leads to structural and functional diversiﬁcationof...

Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx

... integrin-dependentcell volume sensing and signaling integrates into theoverall context of insulin signaling. Similarly, sensing of glutamine-induced hepatocyte swelling by integrinsfeeds into ... outcome.This minireview summarizes recent progress in the understanding of howosmosensing and osmosignaling integrate into the overall context of growthfactor signaling and the execution of apoptotic ... FEBSMINIREVIEWOsmosensing and signaling in the regulation of mammalian cell functionFreimut Schliess, Roland Reinehr and Dieter Ha¨ussingerClinic for Gastroenterology, Hepatology and Infectiology,...

Tài liệu Báo cáo khoa học: "Liars and Saviors in a Sentiment Annotated Corpus of Comments to Political debates" pdf

... Number of negative and iro5 Main Findings and Future DirectionsWe showed that in our setting negative opinions tend to greatly outnumber positive opinions, leaing to a very unbalanced opinion ... the existing approaches to opinion mining propose algorithms that are independent of the text genre, the topic and the target involved. However, practice shows that the opinion mining challenges ... Opinion Mea-surement, Hong Kong. Ganapathibhotla, Murthy, and Bing Liu. 2008. “Mining Opinions in Comparative Sentences”. In Proc. of the 22nd International Conference on Computational Lin-guistics,...

Tài liệu Báo cáo khoa học: "Abstraction and Generalisation in Semantic Role Labels: PropBank, VerbNet or both?" doc

... ting Yi, and Martha Palmer. 2007.Combining lexical resources: Mapping betweenPropBank and VerbNet. In Proceedings of theIWCS.Christopher Manning and Hinrich Schuetze. 1999.Foundations of ... aspects of a sentence meaning. Capturing thenature and the number of semantic roles in a sentence is therefore fundamental tocorrectly describing the interface betweengrammar and meaning. In this ... of previous work. In some interesting experiments, Zapirain et al.(2008) test generalising abilities of VerbNet and PropBank comparatively to new role instances in general (their Table 1, line...

Tài liệu Báo cáo khoa học: " MISREPRESENTATION AND CONTEXT IN DIALOGUE" doc

... representation, or the instructional plan. The individual conditions are combined to determine a final "score" for the PMM, using a calculus akin to MYCIN's certainty factors ([Shortliffe ... information when needed. Input to the system is a teaching situation including information from the content planner, the student model, and the domain. The system's output is a log of ... University of Pittsburgh, 1991-92 differential equations are left out of engineering classes because of their complexity. Simplified and imperfect representations are often found in tutoring discourse....

Tài liệu Báo cáo khoa học: "CODES AND CLUES IN CONTEXTS" pdf

... is inferred from clues provided by code and context. Both kinds of meaning are related to syntax in ways that we are coming to understand better as work continues in analyzing language and ... appropriateness of answers, and reports an investigation of the extralinguistic conceptual structuring of space that affects the pragmatic rules people follow in furnishing appropriate information in ... proceeds, and the use of focusing (an extrallngulstlc prc~ess) tq control the inferences made in identifying the referents of pronominal anaphora. Levin and Hutchlnson analyze the clues in reports...

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