báo cáo khoa học: " Surface structure, model and mechanism of an insect integument adapted to be damaged easily" pot

... Central Page 1 of 11 (page number not for citation purposes) Journal of Nanobiotechnology Open Access Research Surface structure, model and mechanism of an insect integument adapted to be damaged easily Jean-Luc ... surface, to consider it from a mechanistic point of view, and to discuss potential consequences of the integument surface in the preda...

Ngày tải lên: 11/08/2014, 00:22

... STAT5-HA-JH1 displayed specific and strong binding to the ST5 probe (Fig. 5B, lanes 2, 3, and 4) and Int16 probe (Fig. 5B, lanes 6, 7, and 8) which has been found in intron 16 of the T. fluviatilis JAK1 ... incubated with labelled probes ST5 (lanes 2–4), and Int16 (lanes 6–8), followed by EMSA. In vitro transcription and translation product of pu-STAT5-HA was used as controls...

Ngày tải lên: 31/03/2014, 07:20

... control of nondegraded [ 3 H]gelatin, and lane 3 shows a positive control of trypsin digested [ 3 H]gelatin. In lane 5, the bands at 30 kDa and below appear as two spots instead of a band due to a ... 74/76 kDa doublet and 80 and 85 kDa forms of gelatinase in the CaCl 2 treated material. (B) The amount of TIMP-1 used was 1 (lane 3), 10 (lane 4) and 20 n M (lane 5). In (A...

Ngày tải lên: 17/03/2014, 10:20

... the transfer of the C-6¢ pro-R hydride of UDP-glucose to the si face (B face) of NAD + to form NADH and an aldehyde intermediate [13,14]. This inter- mediate is bound tightly to the enzyme and ... process and the value of A 340 does not return to its original position. This occurs because the UDP- glucose that is generated by reduction of the aldehyde cannot be oxi...

Ngày tải lên: 23/03/2014, 12:20

... xylanase As XAIP shows significant sequence identity and con- siderable structural similarity with XIP-I [9,13], which is an inhibitor of GH10 and GH11 xylanases, the role of XAIP as an inhibitor ... centrifuged at 1816 g, and the absorbances of the supernatants were recorded at 575 nm at intervals of 2 h. Xylanase inhibition assay Xylanase from P. furniculosum and beechwoo...

Ngày tải lên: 29/03/2014, 09:20

... camalexin and 3-phenylindole on the activity of BH from mycelial cultures of Leptosphaeria maculans (A) and Alternaria brassicicola (B). The results are expressed as means and standard deviations of ... 3-indolylmethanamine (3), carbonyl sulphide and methanethiol. The chemical mechanism of dithiocarbamate hydro- lysis catalysed by BHs is expected to be similar to the h...

Ngày tải lên: 18/02/2014, 14:20

... to be able to transfer protons [31]. However, without an overexpression approach of RpCAbr and RpCAtr, we cannot know the functional effect of changes of some key amino-acids. Origin and number ... instead of 5¢ capping recruitment [23]. The occurrence of such a mechanism could enhance the regulation capacity for CA translation and may be correlated to an inhib...

Ngày tải lên: 18/02/2014, 16:20

... ratio of 8 : 2, spotted onto the Anchor-Chip plate and air dried. Peptide spectra over a mass range of m ⁄ z 500–3500 and MS ⁄ MS spectra of select ions were collected on an Ultra- flex II MALDI-TOF ... chaperone, identified in human and yeast 2-Cys Prx [10,11], exhibits a marked sensi- tivity to a variety of compounds and conditions, such as reductants and ROS. An imbal...

Ngày tải lên: 18/02/2014, 17:20

... University. Acknowledgements We would like to thank Rongguang Zhang and And- rzej Joachimiak (Advanced Photon Source, Argonne) for help with data collection, and Xiaodong Zhao (ZR group) for technical assistance. This work ... membrane. All SDS/PAGE bands in the gel were then transferred onto nitrocellulose membrane by elec- trophoresis for 2 h at 40 V. The membrane was dyed with Coomas...

Ngày tải lên: 19/02/2014, 02:20

... yet prove to be an important feature of some a-conotoxins in the understanding of structure-function relationships and the interaction of these ligands with the nAChR. Identification of PTMs Methods ... under standard conditions because of the lability of the extra carboxyl group ()44 Da) ([5,41] and as shown in Fig. 1). This residue can not be reliably identified by sta...

Ngày tải lên: 19/02/2014, 12:20