báo cáo khoa học: "mRNA analysis of single living cells" docx

... mammalian single cells. Our studies Table 1: The detection of β-actin mRNA from single VNOf90 cells. The number of assays against single cells The number of β-actin positive cases The number of cells ... change of β-actin mRNA levels was also small compared with that of c-fos mRNA levels in single cell analysis as well as in the cell population analysis. Although the...

Ngày tải lên: 11/08/2014, 00:21

... expression analysis Microarray [58] RNA-seq [93] miRNA expression analysis Microarray [11] RNA-seq [18] lncRNA expression analysis Microarray [12,13] Identication of alternative splicing isoforms ... experimental means of verifying the relationship of SNPs or haplotypes with diseases. Abbreviations CHARGE, Coloboma of the eye, Heart defects, Atresia of the choanae, Retarda...

Ngày tải lên: 11/08/2014, 12:21

... time-dependent mRNA expression of single living cells, but it is limited to the analysis of the fine localization of mRNA in the cells. ISH can examine mRNA expression of the whole cells with higher ... Ikai Atsushi: mRNA analysis of single living cells. Journal of Nanobiotechnology 2003, 1(2):1-8. 13. Uehara Hironori, Osada Toshiya, Ikai Atsushi: Quantitative meas- ur...

Ngày tải lên: 11/08/2014, 00:22

... Department of Structural and Functional Biology, and Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy 2 Department of Biotechnology, University of Verona, Italy 3 Department of Clinical ... specifically linked to one of the major patho- genetic mechanisms of PD [5]. Worthy of note is the complete disappearance of the VDAC-2 upon dopa- mine treatment. Thi...

Ngày tải lên: 15/02/2014, 01:20

... re-plot of Dslope )1 of the Lineweaver– Burk plot as a function of the reciprocal of the benzalkonium concentration. (B) Mutant D70G:d, no benzalkonium; s,2l M; , 3 l M; h,4lM. Modulation of butyrylcholinesterase ... hydrolysis of aryl acylamides by BuChE [33]. However, several lines of structural evi- dence clearly rule out this hypothesis. First, kinetic analysis of organo...

Ngày tải lên: 18/02/2014, 17:20

... thylakoids in the absence of additives or in the presence of 1 lm DCMU or dPQ over a range of temperature from 15 °Cto25°C. The range of tem- perature was set on the basis of the membrane trans- ition ... superposition of the exponential rise to analyze quantitatively the contribution of each phase. The amplitude and rate constant of each of the three phases can be calculate...

Ngày tải lên: 19/02/2014, 05:20

... (in subunits). Inset: plo t of 1/DA as a function of the reciprocal total pro tein concen tration. T he reciproca l of the Y-in ter- cept of this plot gave th e value of DA 1 , the change in a ... excess of CuTCPP and C uTMPyP are also shown. A fi t of the CD spectrum of native MCL, obtained by analysing the spectrum using the CDSSTR program, is also given (details of the spe...

Ngày tải lên: 19/02/2014, 16:20

... viscosity of t he extract as a result of D NA, 2 lLof DNase I (200 units) was added per 100 lL of buffer [30]. Following filtration through two layers of miracloth a nd the addition of four volumes of ... ÔStains- AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4. As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional...

Ngày tải lên: 19/02/2014, 16:20

... S DS/PAGE with 10% of input 35 S-labelled DEC1, and visualized by autoradiography. Ó FEBS 2004 Interaction of DEC1 with BMAL1 (Eur. J. Biochem. 271) 4417 Functional analysis of the basic helix-loop-helix ... Kato 1 1 Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan; 2 Department of Physiology, Hokkaido U...

Ngày tải lên: 19/02/2014, 16:20

... Formation of the covalent serpin– protease complex involves a Michaelis docking complex, cleavage of the P 1 –P 1 ¢ peptide bond, linkage of the active site Ser of the protease to the carboxyl group of ... Mechan- isms of conversion of plasminogen activator inhibitor 1 from a suicide inhibitor to a substrate by monoclonal antibodies. J. Biol. Chem. 277, 43858–43865. Ó FEBS 2003...

Ngày tải lên: 20/02/2014, 11:20