báo cáo khoa học: "Self-assembly of proteins and their nucleic acids" docx
Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... breakthrough in the field of proteo-mics and enables rapid sequencing of large peptides and intact proteins on a QLT mass spectrometer. Thescan times required for direct analysis of proteins usingETD ... Fragmentation scheme for production of ions of type c¢ and z¢Æby reaction of a low-energy electron with a multiply protonatedpeptide.ETD-MS analysis of peptides and proteins N. D. Udeshi et al.6270 ... building blocks of eukaryotic chromatin[15]. A host of PTMs reside on the N-terminal tails of the histones, and this array of PTMs has been welldocumented and includes monomethylation and Fig. 2....
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báo cáo khoa học: "Self-assembly of proteins and their nucleic acids" docx
... 1 of 16(page number not for citation purposes)Journal of NanobiotechnologyOpen AccessResearchSelf-assembly of proteins and their nucleic acidsGraham Fletcher, Sean Mason, Jon Terrett and ... fragment of theSAPV was amplified using 3 ul of 16 pmol/ul M13F prim-er (part of the TOPO vector, upstream of the T7-F primer of the SAPV insert), 0.5 ul of 100 pmol/ul SA-11R primer and 0.5 ul of ... then nucleic acids should in-clude binding sites (i.e. specific sequences) for nucleic acid-binding proteins and should also encode corre-sponding nucleic acid-binding proteins. The use of knownprotein...
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báo cáo khoa học: " Identification of miRNAs and their target genes in developing soybean seeds by deep sequencing" doc
... correspondingtarget genes and study of their roles will elucidate possi-ble functions of miRNAs and target genes in relevantprocesses of seed development.Figure 8 GO analysis of targets of known and new miRNAs ... Pentatricopeptide repeat proteins (PPR) were detected as the targets of Soy_3 and Soy_16.PPR-containing proteins perform functions at the post-transcriptional level in mitochondria and chloroplasts and are ... numbers of miRNAs and their related target genes through deepsequencing of a small RNA library and a degradome library. Our study provides more information about theregulatory network of miRNAs...
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Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... attention was given to the bandingpatterns and different glycoforms of PrPC. In thisstudy, we have analyzed the glycoform patterns of PrPC of human, sheep, cattle and mice and comparedthem. Variable ... nonglycosylated isoform (m) of PrPCwere quantified and calcu-lated as percentages of the total signal. The glycoforms of the pro-tein bands were analyzed as arithmetic means of separate gelruns. ... the protein bands were analyzed by calcula-tion of the percentages of the di- (d), mono- (j) and nonglycosy-lated (m) isoform as arithmetic means of separate gel runs. Thenumber of gel runs...
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Tài liệu Báo cáo khoa học: Mechanisms of obesity and related pathologies: Androgen deficiency and endothelial dysfunction may be the link between obesity and erectile dysfunction pptx
... pathophysiologicalmechanisms of obesity-induced androgen deficiency and hypogonadism-induced obesity remain the subject of intense investigation.Several hypotheses have discussed the relationship of androgen deficiency and ... FEBSMINIREVIEWMechanisms of obesity and related pathologies: Androgendeficiency and endothelial dysfunction may be the linkbetween obesity and erectile dysfunctionAbdulmaged M. Traish1, Robert J. Feeley1 and Andre ... pathophysiologicalmechanisms of endothelial dysfunction and testosterone deficiency includepenile vascular insufficiency as a result of the loss of nitric oxide synthaseexpression and activity and the loss of tissue...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... Institute of Plant Sciences, Department of Agricultural and Food Sciences and Department of Biology, Zu¨rich, Switzerland;2Universita¨tZu¨rich, Institute of Biochemistry, Zu¨rich, SwitzerlandUDP-N-acetylglucosamine ... [9]), and closed(B, PDB entry 1UAE [11]), conformation. Thethiol group of C115 is highlighted in green and the nitrogen atoms of R120 and K22 in blue.The open form of MurA is unliganded (A) and the ... biosynthesis of UDP-N-acetylmuramic acid) (A) and inactivation mechanism of MurA byfosfomycin as a result of the covalent linkage between Cys115 of MurA and fosfomycin (B).Ó FEBS 2004 Lysine 22 and...
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Tài liệu Báo cáo khoa học: "Order of Subject and Predicate in Scientific Russia" pptx
... variety of classes of words. Almost 1/4 of the clauses contain no subject. The results of the above study indicate that the word order in scientific Russian is sufficiently different from that of ... Order of Subject and Predicate 67 The clause contained no subject in 657 in- stances, or 22.55% of the total. 1. The predicate slot was filled by a verb in 1527 instances, or 52. 40% of ... 7.96% of the total. The word order was Subject - Predicate in all instances without exception. 3. The predicate slot was filled by an adjective in 496 instances, or 17. 02% of the total. Of...
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Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafficking pdf
... molecularweights of approximately 52, 40 and 36 kDa.Effect of deletions corresponding to cleavage and shedding comparative to a deletion of almostthe whole extracellular domain of the receptoron ... extracellular and a b-transmembrane and intracellularsubunits, held together by disulfide bonds. Moreover, part of the a-subunit of the receptor is shed from thyroid and transfected L cells. To understand ... ResultsGeneration of truncated mutants corresponding eitherto b-subunits of the TSHR, or to a receptor deleted of almost the entire extracellular domainTo understand the role of receptor cleavage and shedding,we...
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Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt
... for different domains of the Lsm1 and Lsm8 proteins in their function and localization, we created a series of mutant and hybrid proteins. We deleted or exchanged their N- and ⁄ orC-terminal domains, ... LSm1 and ABFig. 7. Correlation between viability and correct localization of Lsm1 and Lsm8 hybrid and mutant proteins. (A) Mutant proteins that accumu-late in the nucleus. (B) Mutant proteins ... onalignment and structure predictions, we define theN-terminal domain of Lsm1p as aa 1–51 and thatABCFig. 1. Structural features of Lsm1 and Lsm8 polypeptides. (A) Alignment of Lsm1p and Lsm8p...
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Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt
... g,4°C, 15 min).Purification of recombinant expressed proteins DptE and DptFFor purification of DptE and DptF, cell pellets from 1 litre of culture were resuspended in 10 mL of buffer A (50 mmphosphate ... dimethylsulfoxide and 500 lm decanoic acid. Michaelis–Menten fitting of the experimental data set resulted in a KM of 135 lm and a kcat of 6.3 min)1. The catalytic efficiency of thetransfer ... AMP to cognate acyl carrier proteins (ACPs) [14,15].The genes dptE and dptF are localized immediatelyupstream of the NRPSs of A21987C. The resulting proteins DptE and DptF were predicted to...
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