báo cáo khoa học: "Self-assembly of proteins and their nucleic acids" docx

... breakthrough in the field of proteo- mics and enables rapid sequencing of large peptides and intact proteins on a QLT mass spectrometer. The scan times required for direct analysis of proteins using ETD ... Fragmentation scheme for production of ions of type c¢ and z¢ Æ by reaction of a low-energy electron with a multiply protonated peptide. ETD-MS analysis of peptides...

Ngày tải lên: 18/02/2014, 16:20

... 1 of 16 (page number not for citation purposes) Journal of Nanobiotechnology Open Access Research Self-assembly of proteins and their nucleic acids Graham Fletcher, Sean Mason, Jon Terrett and ... fragment of the SAPV was amplified using 3 ul of 16 pmol/ul M13F prim- er (part of the TOPO vector, upstream of the T7-F primer of the SAPV insert), 0.5 ul of 100 pmol/ul...

Ngày tải lên: 11/08/2014, 00:21

... corresponding target genes and study of their roles will elucidate possi- ble functions of miRNAs and target genes in relevant processes of seed development. Figure 8 GO analysis of targets of known and new miRNAs ... Pentatricopeptide repeat proteins (PPR) were detected as the targets of Soy_3 and Soy_16. PPR-containing proteins perform functions at the post- transc...

Ngày tải lên: 11/08/2014, 11:21

... attention was given to the banding patterns and different glycoforms of PrP C . In this study, we have analyzed the glycoform patterns of PrP C of human, sheep, cattle and mice and compared them. Variable ... nonglycosylated isoform (m) of PrP C were quantified and calcu- lated as percentages of the total signal. The glycoforms of the pro- tein bands were analyzed as arithm...

Ngày tải lên: 19/02/2014, 02:20

... pathophysiological mechanisms of obesity-induced androgen deficiency and hypogonadism-induced obesity remain the subject of intense investigation. Several hypotheses have discussed the relationship of androgen deficiency and ... FEBS MINIREVIEW Mechanisms of obesity and related pathologies: Androgen deficiency and endothelial dysfunction may be the link between obesity and erec...

Ngày tải lên: 18/02/2014, 06:20

... Institute of Plant Sciences, Department of Agricultural and Food Sciences and Department of Biology, Zu ¨ rich, Switzerland; 2 Universita ¨ tZu ¨ rich, Institute of Biochemistry, Zu ¨ rich, Switzerland UDP-N-acetylglucosamine ... [9]), and closed (B, PDB entry 1UAE [11]), conformation. The thiol group of C115 is highlighted in green and the nitrogen atoms of R120 and K22...

Ngày tải lên: 19/02/2014, 13:20

... variety of classes of words. Almost 1/4 of the clauses contain no subject. The results of the above study indicate that the word order in scientific Russian is sufficiently different from that of ... Order of Subject and Predicate 67 The clause contained no subject in 657 in- stances, or 22.55% of the total. 1. The predicate slot was filled by a verb in 1527 inst...

Ngày tải lên: 19/02/2014, 19:20

... molecular weights of approximately 52, 40 and 36 kDa. Effect of deletions corresponding to cleavage and shedding comparative to a deletion of almost the whole extracellular domain of the receptor on ... extracellular and a b-transmembrane and intracellular subunits, held together by disulfide bonds. Moreover, part of the a-subunit of the receptor is shed from thyroid and...

Ngày tải lên: 21/02/2014, 00:20

... for different domains of the Lsm1 and Lsm8 proteins in their function and localization, we created a series of mutant and hybrid proteins. We deleted or exchanged their N- and ⁄ or C-terminal domains, ... LSm1 and AB Fig. 7. Correlation between viability and correct localization of Lsm1 and Lsm8 hybrid and mutant proteins. (A) Mutant proteins that accumu- late...

Ngày tải lên: 07/03/2014, 02:20

... g,4°C, 15 min). Purification of recombinant expressed proteins DptE and DptF For purification of DptE and DptF, cell pellets from 1 litre of culture were resuspended in 10 mL of buffer A (50 mm phosphate ... dimethylsulfoxide and 500 lm decanoic acid. Michaelis–Menten fitting of the experimental data set resulted in a K M of 135 lm and a k cat of 6.3 min )1 . The catalyti...

Ngày tải lên: 07/03/2014, 04:20