báo cáo khoa học: "eneration of diffuse large B cell lymphomaassociated antigen-specific Va6/Vb13+T cells by TCR gene transfer" pdf

... gene- trans- duced CD3+T cells against Toledo cells by cassette bIa Figure 2 TCR V b1 3 protein expression was detected in TCR gene- transfected CD3+T cells by Western Blot analysis. Lane 1: CD3+T cells transfected ... aIb. Cytotoxicity of < /b> TCR gene- transduced CD3+T cells against Toledo cells by two types of < /b> TCR transgene cassettes (aIb, P = 0.008;...

Ngày tải lên: 10/08/2014, 22:21

... Alizadeh AA, Eisen MB, Davis RE, et al.: Distinct types of < /b> diffuse < /b> large < /b> B- cell lymphoma identified by gene expression profil- ing. Nature 2000, 403(6769):503-511. 22. Hans CP, Weisenburger DD, Greiner ... expression of < /b> CD30, CD45, plasma cell, B- cell, T -cell and NK -cell mark- ers, EMA and light chain, heavy chain gene and T -cell receptor gene...

Ngày tải lên: 10/08/2014, 22:20

... Asian variant of < /b> intravascular large < /b> B- cell lymphoma: clinical, pathological and cytogenetic approaches to diffuse < /b> large < /b> B- cell lymphoma associated with haemophagocytic syndrome. Br J Haematol ... Hodgkin lymphoma. Number of < /b> cases with DLBCL and B- IVL was 1,815(44.6% of < /b> all ML, 48.7% of < /b> NHL) and 9(0.22% of < /b> all ML, 0.24% of <...

Ngày tải lên: 10/08/2014, 21:23

... Matsushita K, Abe Y, Rohman MS, Koga Y, Takano K & Kanaya S (2008) Remarkable stabilization of < /b> a psychrotrophic RNase HI by combi- nation of < /b> thermostabilizing mutations identified by sup- pressor ... Several proteins have also been reported to be stabilized by the introduction of < /b> ion pairs [18–21]. Asp39 fi Gly So-RNase HI is stabilized by the Asp39 fi Gly muta-...

Ngày tải lên: 18/02/2014, 13:20

... in thyroid epithelial cells: activation by TSH, inhi- bition by TGFbeta, and phosphorylations of < /b> its subunits demonstrated by two-dimensional gel electro- phoresis. Exp Cell Res 291, 135–149. 35 ... amino-terminal domains in subcellular localization. Mol Biol Cell 10, 3771–3786. 27 Thomas SM & Brugge JS (1997) Cellular functions reg- ulated by Src family kinases. Annu...

Ngày tải lên: 18/02/2014, 18:20

... plasma membrane of < /b> Chinese ham- ster ovary cells [37]. However, assessment of < /b> the sub- cellular distribution of < /b> endogenous ARF-6 in various other tissues and cells has established both a cytosolic and ... [2]. CT belongs to the AB family of < /b> bacterial exotoxins, and consists of < /b> a pentameric B subunit (CT -B) and an A subunit (CT-A) comprising two polype...

Ngày tải lên: 19/02/2014, 02:20

... concentrations of < /b> unlabeled ATP. Nitrocellulose membranes were soaked briefly in 0.4 m KOH, rinsed thoroughly, and mounted on a dot blot apparatus. The binding reaction was stopped by addition of < /b> 70 lL of < /b> ... were separated by SDS ⁄ PAGE, and AB C Fig. 3. ATP inhibits IRP–IRE binding activity. IRE binding activity was measured by EMSA using either cytosolic extract...

Ngày tải lên: 07/03/2014, 09:20

... pUMGPT-ZK-I31A. Construction of < /b> yeast strains The strains used in this study are described in Table 1. The genes were introduced into yeast cells using the lithium acetate method [15]. Substitution of < /b> the STE18 gene by ... media described above to produce YPD and SD-Ura agar. Construction of < /b> plasmids for yeast chromosome substitution Plasmids used for deletion of <...

Ngày tải lên: 16/03/2014, 01:20

... kinetic mechanism of < /b> Scheme 1. The rate constants reported in Table 2 were estimated on the basis of < /b> this method. For the kinetic set-up of < /b> Scheme 1a ,b, the steady state is described by Eqns (1,2), by analogy ... was chosen as the target of < /b> mutagenesis by error–prone PCR [16]. A library of < /b> DAAO mutants was then generated in BL21(DE3)pLysS E. coli cel...

Ngày tải lên: 16/03/2014, 02:20

... per- formed by transferring the proteins on to nitrocellulose membrane as described by the manufacturer (Novex). Subsequently, the blots were blocked with NaCl/P i /1% BSA and incubated o vernight ... also recognized by these polyclonal rabbit antibodies. The total cleavage product was subsequently radiolabeled and sepa- rated by size-exclusion chromatography. Four peaks were detected...

Ngày tải lên: 17/03/2014, 11:20