... 529–540.22 Shimomura Y, Honda T, Shiraki M, Murakami T,Sato J, Kobayashi H, Mawatari K, Obayashi M &Harris RA (2006) Branched-chain amino acidcatabolism in exercise and liver disease. J Nutr ... mutant exhibits abnormally high accumu-lations of branched-chain amino acids, includingleucine, in haemolymph [6]. From the second feature,it can be assumed that the branched-chain amino aciddegradation ... 2,6-dichloroindophenol were obtained from Wako PureChemical Industries (Osaka, Japan) and isovaleryl-CoA,isobutyryl-CoA and hexanoyl-CoA substrates wereobtained from Sigma-Aldrich (St. Louis, MO, USA).Comparative...