báo cáo khoa học: " Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells" potx
báo cáo khoa học: " Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells" potx
... AND MICB increased during the first five days in culture (Figure 6).CALO and INBL proliferate in response to MICA and MICB After we detected the expression of MICA, MICB, and NKG2D in CALO and ... possible expression of these proteins in two human epithelial cervical cancer cell lines, CALO and INBL, using polyclonal antibodies against MICA /MICB and anti -NKG2D for western blot and flow ... doubling times in vitro because the cervical cells had a doubling time of Figure 4 Cervical cancer cell lines express MICA, MICB and NKG2D. CALO and INBL cells (1×107) were lysed proteins immunoprecipitatedand...
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Báo cáo y học: "Expression of cartilage-derived morphogenetic protein in human intervertebral discs and its effect on matrix synthesis in degenerate human nucleus pulposus cells" potx
... 2Assessment of immunopositive staining for CDMP 1 and 2 in human intervertebral discsAssessment of immunopositive staining for CDMP 1 and 2 in human intervertebral discs. Percentage of cells with ... wasobserved in the matrix of the IVD or in the endothelial cells of Figure 1Examples of immunohistochemical staining for CDMPs in human intervertebral discExamples of immunohistochemical staining for ... and bovine disccells in alginate [21] have shown significant increases in cellproliferation. Here we showed a small increase in proliferation of human disc cells in alginate culture following...
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Báo cáo khoa học: Expression of heme oxygenase-1 is repressed by interferon-c and induced by hypoxia in human retinal pigment epithelial cells pot
... determinant for the hypoxia-mediated induction of HO-1 expression in D407 cells. In addition, hypoxiacoordinately and rapidly induced expression of both HO-1 and Bach1 mRNAs in cultured rat and ... geneity of R PE cells [31,48].Effects of hypoxia on the expression of HO-1 in human RPE cell lines In contrast to interferon-c, hypoxia indu ced the expression levels of HO-1 mRNA in D407 cells and ... effects of interferon-c or hypoxia on HO-1 expression in human RPE cells. Here we show the repression of HO-1 expression by interferon-c and the induction of HO-1 expression by hypoxia in human RPE...
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Báo cáo khoa học: "Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development" ppsx
... (eNOS), and inducible (iNOS) isoforms of nitric oxidesynthase (NOS) in the ovaries of pigs. The intensity of staining is indicated by (-), where staining was absent, up to (+++), for maximalstainingTissue ... multilaminar and antral follicles, but not in those of unilaminar follicles.iNOS was detected in the surface epithelium, oocytes, and theca of multilaminar and antral follicles. Taking all of thefindings ... stages,including Graafian follicles, immunostaining for threeisoforms of NOS was detected in the granulosa and thecacells. This finding suggests that, in the porcine ovary,granulosa and theca...
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Báo cáo khoa học: Downregulation of protease-activated receptor-1 in human lung fibroblasts is specifically mediated by the prostaglandin E2 receptor EP2 through cAMP elevation and protein kinase A pot
... regulator of the PAR-1 gene and acompetitor of AP-2 for binding to the regulatoryregion of the PAR-1 gene [19,29]. Interestingly, in the human alveolar epithelial A549 cell line, Sp1 inhibi-tion, ... to in uence the basal gene expression of PAR-1 in other cell lines expressing this receptor. Weused the human astrocytoma cell line 1321N1 and the human alveolar epithelial cell line A549. In ... participate in wound healingafter tissue injury and in inflammatory responses byproduction of a vast variety of proinflammatory medi-ators, growth factors, and extracellular matrix compo-nents. Many of...
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báo cáo khoa học: "Activation of stress-related signalling pathway in human cells upon SiO2 nanoparticles exposure as an early indicator of cytotoxicity" pps
... of clinical medicine, Institute of Molecular Medicine, TrinityCollege Dublin, Dublin8, IrelandFull list of author information is available at the end of the articleMohamed et al. Journal of ... relevantmodel of the in vivo first line of interaction betweennanoparticles and human tissues, as it would beexpected following exposure of the lungs (inhalation) and uptake of foreign material ... causeabnormal clusters of topoisomerase I in the nucleoplasm of cells, and pro-inflammatory stimulation both in vivo and in vitro [26-29].Lin et al. [30] demonstrated in an in vitro study thatamorphous...
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Báo cáo khoa học: " Profile of time-dependent VEGF upregulation in human pulmonary endothelial cells, HPMEC-ST1.6R infected with DENV-1, -2, -3, and -4 viruses" pps
... VEGF and activation of angiogenesis. In this study, increase in VEGF expression level at designated time points of 0, 8, 24, 96 and 192 hours post-infection wasinvestigated, using a microbead-based ... TNF-α, TGF-β, insulin-like growth factor 1, IL-1β, IL-6 and PGE2 [11]. We and others have shown that expression of some of these cytokines become increasedas a consequence of infection by DENV ... immunoassay. Increased level of VEGF expression in infected-HPMEC-ST1.6R was detected at 8 hours post-infection. Interestingly, VEGF expression level began to decrease up to 96 hours post-infection,...
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Báo cáo y học: " Expression of Toll-like Receptor 9 in nose, peripheral blood and bone marrow during symptomatic allergic rhinitis" ppsx
... the expression of TLR9 in human bone marrow leukocytes. In nasal lavagefluid, neutrophils displayed an intracellular expression of TLR9.The expression of TLR9 in neutrophils, eosinophils and basophils ... immuno-therapy in humans [41]. Thus, the distinct and general expression of TLR9 in the nasal epithelium and in variousleukocytes in the nasal mucosa, indicates that bacterial and viral airway infections ... content of CpG and this could represent one pathogenic mechanism explain-ing the worsening of allergic inflammation during airwayinfections.ConclusionThe widespread and rich expression of TLR9 in...
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Tài liệu Báo cáo khoa học: Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells doc
... basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and aHECT ubiquitin ligase. EMBO J 23, 272–281.9 Craig A (1998) Interaction of polyadenylate-bindingprotein ... lg of plasmid DNAusing 10 lg of lipofectamine (Invitrogen, Burlington,Canada), according to protocols supplied by the manufac-turer. In short, the DNA was mixed with lipofectamine and PABP expression ... that, in addition to the C-terminal domain, PABP1has another eIF4G-binding domain within its N-termi-nal RNA-binding domain 1 [27]. As a negative control,we also examined the colocalization of...
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