Báo cáo y học: "proteomic characterization of non-small cell lung cancer in a comprehensive translational thoracic oncology database" docx

... staining compares the relative intensity of staining of onecoreofaTMAtothatofacontrolcoreonthe same slide. A score of 1 indicates faint staining, 2 indi- cates medium intensity staining, and ... laboratory and clinical data regarding patient specimens to be maintained separately, the storage of such heterogeneous data in one database offers many benefits as it may facilitate more...

Ngày tải lên: 10/08/2014, 09:22

... line immunoblot assays (LIAs) for single-parameter or profile anal- ysis – have been established in clinical laboratory practice dur- ing past years, allowing examination of a large number of sera in a rapid ... performed using alkaline phos- phatase-labelled goat anti-human IgG. Control sera were included in each assay. Incubated blot strips were digitalized using a flatbed s...

Ngày tải lên: 09/08/2014, 01:22

... Fujino S, Andoh A, Bamba S, Ogawa A, Hata K, Araki Y, Bamba T, Fujiyama Y: Increased expression of interleukin 17 in inflam- matory bowel disease. Gut 2003, 52:65-70. 68. Katz Y, Nadiv O, Beer Y: ... disease, and T cells comprise a large proportion of the inflammatory cells invading the synovial tissue. T cell activation and migration into the synovium occur as an early conseq...

Ngày tải lên: 09/08/2014, 06:22

... arteria mammaria interna and a compar- atively thin RCA. To lessen the risk of a relapse, the implantation of an automatic implantable cardioverter defibrillator (AICD) was indicated. The athlete ... long-distance runner collapsed at the finish of a half marathon because of a myocardial infarction with ventricular fibrillation. Coronary angiography showed an anomalous origin...

Ngày tải lên: 11/08/2014, 23:21

... 2. Materials and methods Cell line and cell cultures The human choricarcinoma cell line JAR was obtained from American Type Culture Collection (Manassas, VA, USA). Cells were maintained in ... apoptosis-related proteins induced by hypoxia in trophoblastic cells so as to clarify the mechanism of hypoxia-induced apoptosis by using the PoweBlot, an antibody-based Western array...

Ngày tải lên: 31/10/2012, 15:12

... The addition of a flavone (apigenin), of a flavanonol (taxifolin) and of a flavan-3-ol (epicatechin) (Fig. 8) did not alter the EPR spectra of the enzyme indicating the absence of binding to the copper ... BamHI–HindIII fragment from pAW4-1 containing the A. awamori pyrG gene as selection marker. Transformants were screened for extra- cellular production of 2,3QD in a plat...

Ngày tải lên: 22/02/2014, 07:20

... is a measure of acyl chain order. HDL induces a slight rigidification in particular in the liquid crystalline (a) phase of the acyl chains of LPS Re, as deduced from a decrease in wavenumber values ... 1), to an increase of the phase transition temperature of the acyl chains of the endotoxins and a drastic increase in acyl chain order, i.e. a rigidification of th...

Ngày tải lên: 17/03/2014, 10:20

... antibodies against annexin II strongly stained the plasma membrane in motor neu- rons in the lumbar spinal cord of G9 3A mice, whereas mostly weak nuclear and cytoplasmic staining was observed in WT ... functions, such as vesicular transport, neurotransmitter synthesis and release; cytoskeletal organization, and linkage to the plasma membrane; metabolism; and microglia activation and...

Ngày tải lên: 23/03/2014, 04:21

... scintillant (Amersham). Each value is an average of four independent colonies and the assays were performed in triplicate. b-Galactosidase assay b-Galactosidase activity was assayed in cell extracts as described ... Growth of a wild-type strain bearing the CYC1::GAL1 construct which expresses galactokinase at a basal level on glycerol plus lactate was marginally reduced in the...

Ngày tải lên: 31/03/2014, 08:20

... Polyadenylated mRNA was purified by oligo(dT)-cellulose chromatogra- phy (Amersharm Pharmacia). A cDNA library was con- structed using a ZAP-cDNA Synthesis kit (Stratagene) and a Gigapack in vitro ... replicate only in ovarian calyx cells of the female wasps. Despite the absence of replication in the parasitized hosts, PDVs induce an array of physiological alterations that ar...

Ngày tải lên: 31/03/2014, 15:20