Báo cáo y học: "Functional characterization of Trip10 in cancer cell growth and survival" ppsx

... Overex- pression of Trip10 in IMR-32 cells caused Trip10 and huntingtin to colocalize and form perinuclear foci. In contrast, while overexpression of Trip10 in CP70 cells also increased huntingtin level ... with Trip10- overexpressing IMR-32 cells (Figure 4A). On the other hand, huntingtin increases cell death by promot- ing apoptosis. Thus, high levels of huntingtin...

Ngày tải lên: 10/08/2014, 05:21

... bias in assignment of treatment by a physician. ‘Confounding by indication’ means that certain treatments are preferentially given to sicker patients and certain treatments preferentially to ... in improving the use of databanks. Many of the problems with observa- tional studies can be minimized with careful planning in advance of the study. Ideally the subjects in longitudi...

Ngày tải lên: 09/08/2014, 01:23

... 1 Department of Cardiothoracic Surgery, School of Medicine, University of Patras, Greece and 2 Department of Anaesthesiology and Critical Care Medicine, School of Medicine, University of Patras, ... presence of nodal involvement. In stage I surgery must always be followed by adjuvant chemotherapy, while in stage II and III surgery must be planned only in the conte...

Ngày tải lên: 10/08/2014, 10:20

... Nidetzky Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Austria Introduction In terms of their physiological functions, which include enzymatic catalysis, ... Gillingham, UK). Production of superoxide by xanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto- chrome c being reduced by...

Ngày tải lên: 18/02/2014, 06:20

... three domains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64. Cytotoxicity was analyzed in vitro using C2C12 skeletal muscle cells. Toxins ... residues phenylalanine and tyrosine typical of the Ig-fold are bold in each domain. Fig. 5. Inhibition of in vivo myotoxicity of myotoxins I or II by DM6...

Ngày tải lên: 21/02/2014, 01:21

... standard curve was prepared by electrophoresing different amounts of cell free extract from Artemia cysts containing p26 in 12.5% SDS polyacrylamide gels, blotting t o nitrocellulose and probing ... (1992) Expression and aggre- gation of recombinant aA-crystallin and its two domains. Bi ochim. Biophys. Acta 1130, 267–276. 8. Augusteyn, R.C. (1998) a-Crystallin polymers and pol...

Ngày tải lên: 22/02/2014, 04:20

... Egmond 1 and Martina Huber 3 1 Unilever Research Vlaardingen, the Netherlands; 2 University of Groningen, Laboratory of Biophysical Chemistry, Groningen, the Netherlands; 3 Department of Molecular Physics, ... Functional analysis of the copper dependent quercetin 2,3-dioxygenase. 1. Ligand-induced coordination changes probed by X-ray crys- tallography: inhibition, ordering effects a...

Ngày tải lên: 22/02/2014, 07:20

... FAs have a key role in maintaining the correct membrane fluidity in poikilothermic organisms, which is necessary for the mobility and function of embedded proteins and in giving shape to membrane curvatures, ... homology. Moreover, they indicate that a com- plete pathway for very-long-chain polyunsaturated fatty acid biosynthesis is functional in L. major using D6, D5 and D4 de...

Ngày tải lên: 07/03/2014, 12:20

... Groningen, the Netherlands) in order to obtain a recombinant protein containing the C-myc epitope followed by six histidines at the C-terminus. A plasmid containing the wild type version of CCR5 ... concentration; insertion of detergent into the membrane should result in a gradual decrease of RET [41,42], and lysis of the liposomes in abrupt abolition of RET. A decrease in...

Ngày tải lên: 08/03/2014, 09:20

... (1997) The crystal structures of Sinapis alba myro- sinase and a covalent glycosyl±enzyme intermediate provide insights into the substrate recogn ition and active-site machinery of an S-glycosidase. ... 2002 20 lL of the enzyme/inhibitor mixture to 180 lLof substrate (4NPGlc), incubating for 30 min at 37 °C, and measuring the rel ease of 4NP. Protein assays and protein sequenc...

Ngày tải lên: 08/03/2014, 16:20