báo cáo khoa học: "Interaction génotype-milieu et modèle mixte" ppt

... espérances et les covariances sont fonctions de l’environnement. Le modèle (I) contient le modèle (II) et permet d’expliciter par identification le contenu des paramètres du modèle ... conclusions Le modèle d’échantillonnage proposé (modèle (I)) (avec M et P infiniment grands) permet de justifier par des arguments d’échantillonnage simples l...

Ngày tải lên: 09/08/2014, 22:23

... celle en N et N2. Lors- qu’il représente moins d’un tiers ou entre un tiers et deux tiers du mélange, le modèle en N et N2 n’est pas meilleur que le modèle linéaire. Mais ... curvili- néaire. Partant de cette hypothèse, et par souci d’homogénéité, nous avons adopté le modèle curvilinéaire en N et N2 pour les 4 situations (peuplement...

Ngày tải lên: 09/08/2014, 03:24

... full-length v-KIND; DKIND1, deletion of KIND1; DKIND2, deletion of KIND2; DKIND1 + 2, deletion of both KIND1 and KIND2; DRasN, deletion of RasN; DGEF, deletion of RasGEF; KIND1, KIND1 domain; KIND2, KIND2 ... Thus, it would be interesting to deter- mine whether a hydrophobic interaction between the Leu residues from v-KIND and MAP2 contributes to the interaction between the two proteins. In c...

Ngày tải lên: 14/02/2014, 19:20

... Ultra- violet fluorescence of actin. Determination of native actin content in actin preparations. FEBS Lett. 62, 4–6. 24. Kouyama, T. & Mihashi, K. (1981) Fluorimetry study of N-(1- pyrenyl) iodoacetamide-labelled ... of the temperature up to 80 °C had no effect on parameter A. The data presented agree with the results of Kuznetsova et al. [6] and Turoverov et al.[5] who showed that re...

Ngày tải lên: 20/02/2014, 23:20

... of total protein, were diluted with detergent-containing buffer [NET buffer I: 50 mm Tris ⁄ HCl, pH 7.0, 150 mm NaCl, 0.1% (v ⁄ v) nonionic detergent Nonidet P-40, 1 mm EDTA] to a total volume ... sediment was washed twice with NET buffer I, once with NET buffer II (NET buffer I with 500 mm NaCl), and once with buffer IP [10 mm Tris ⁄ HCl, pH 7.5, 0.1% (v ⁄ v) Noni- det P-40]. The bound prote...

Ngày tải lên: 06/03/2014, 00:21

... amplified from a fetal brain cDNA library (Clontech Laboratories, Inc., San Diego, CA) and cloned into pTL1 (EcoRI–BamHI sites), pET–TEV (NdeI–BamHI sites) and pET–GST–TEV (NcoI–BamHI sites). pTL1, pET–TEV ... marker. pET–TIPRL and pET–GST–TIPRL encode N-terminal hexahistidine and GST fusions, respectively, separated by a TEV protease cleavage site. Construction of plasmids pET28a–a4 and pET28...

Ngày tải lên: 07/03/2014, 05:20

... pellet was resus- pended in 15 mL of ice-cold buffer A [20 mm Tris/HCl, pH 7.9, 10% glycerol, 0.5 m NaCl, 5 mm imidazole con- taining protease inhibitors (1 mm phenylmethanesulfonyl Link between ... the conjugation of genetically variable gametes, which are generated during meiosis in the parental germline. Mei- osis consists of two rounds of chromosome segrega- tion, resulting in gametes wit...

Ngày tải lên: 07/03/2014, 05:20

... added. Calpain was detected using a peroxidase-conjugated secon- dary antibody [42] developed with an ECL Ò detection sys- tem (Amersham Pharmacia Biotech). Immunoreactive material was detected by subjecting ... spotted on a nitrocellulose sheet (0.5 cm · 0.5 cm; Bio-Rad Laboratories, Bio-Rad Ita- lia, Milan, Italy) and left for 15 min at 4 °C in a humidified chamber. The sheet was then washed...

Ngày tải lên: 07/03/2014, 12:20

... mean residue ellipiticity at 222 nm [33]. TFE, trifluoroethanol. % a-Helix % b-Structure % Random oil h 222 K2D CONTIN b-Sheet (K2D) b-Sheet (K2D) b-Turn (CONTIN) K2D CONTIN E2(7–26) Buffer 13 ... of E2 located between residues 279 and 298 has both pre- dictive features. The secondary structure of both peptides was meas- ured by CD. We monitored several parameters that determine peptide–membra...

Ngày tải lên: 07/03/2014, 17:20

... using the 3-(4,5- dimethylthiazol- 2-yl)-5-( 3-carboxymethox yphenyl)-2- (4-sulfophenyl)-2H-tetrazolium (MTS) assay, with stefin A as a negative control. Stefin B exhibits a weak, yet significant, surface-seeking ... preparations are toxic to neuroblastoma cells, as determined by the 3-(4,5-dimethylthiazol-2-yl)-5- (3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay, again in contras...

Ngày tải lên: 07/03/2014, 17:20