... reaction(s). Fate of the adenosyl group of AdoCbl in inactivation of a mutant holoenzyme during catalysis To study the fate of the adenosyl group, the upper axial ligand of AdoCbl, in the inactivation of the Sa224A ... bonding of N3 with the side chain OH group of Sera224, and of 6-NH 2 , N1 and N7 with main chain amide groups of other residues....
Ngày tải lên: 23/03/2014, 06:20
... domain to be a consequence of lipid mixing within these microdomains, resulting in the presence of small amounts of SM and cholesterol in the DOPC micro- domain. When 1 mm zinc was present, an increase ... binding, resulting in shifts to larger angles (inset to B) and binding at higher con- centrations resulting in shifts to smaller angles. Binding to the SM-rich domai...
Ngày tải lên: 30/03/2014, 11:20
Báo cáo khoa học: "Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System" ppsx
... supple- mented in culture media on the in vitro development of bovine embryos, and to examine the effects of glucose, phosphate and citrate upon the developmental frequency of bovine embryos. Materials and ... 3. Effects of glucose and/ or phosphate in the tw o-step culture system The effects of glucose and/ or phosphate were examined in Effects of Protei...
Ngày tải lên: 07/08/2014, 17:22
Báo cáo khoa học: " Effects of endomycorrhizal development and light regimes on the growth of Dicorynia guianensis Amshoff seedlings" pptx
... (HLI) and the values of the soil temperature in the pots ranged from 42 to 47 °C. The soil temperature recorded under the two other tunnels (i.e. 1% and 14% of full sunlight) was in the range of ... October 1997, the leaflets of the seedlings were counted every 8–12 days and the height of their stem measured from the soil level to the apical meristem,...
Ngày tải lên: 08/08/2014, 14:22
Báo cáo khoa học: "Effect of article genotype and cutting type on the vegetative propagation of the pine hybrid (Pinus brutia (Ten) x Pinus halepensis (Mill)" pptx
... exists within buds between uxin and endogenous cytokinin, which on several occasions results in the dominance of the apical bud (higher auxin level). Growth of the inhibited ... regardless of the cutting type and hormone level. Several other workers have also noticed the effect of genotype on rooting of conifer cuttings (Thulin and...
Ngày tải lên: 08/08/2014, 19:21
Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... Ets-1 and Egr-1 are substrates of MAPK [12,43,44], the inhibition of MAPK by the incorporation of n-3 PUFAs could alter the phosphorylation of these transcription factors, affecting their binding ... doi:10.1111/j.1432-1033.2004.04385.x determine whether the incorporation of PUFA also affected the concentration of the p42/44 MAPK in caveolae. Pretreatment with EPA...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: "Responses of growth, nitrogen and carbon partitioning to elevated atmospheric CO concentration in live oak 2 (Quercus virginiana Mill.) seedlings in relation to nutrient supply Roberto" pot
... min after the fans were turned off in the evenings in order to maintain higher concentrations in the chambers. [CO 2] was mea- sured continuously in both the ambient and ... cm 3) and maintained in the growth cham- ber. The tubes were filled with a mixture (v/v) of 90 % sand and 10 % peat; a layer of stones was placed in...
Ngày tải lên: 08/08/2014, 14:21
Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx
... Least squares superposition of the P-loop main chain (starting at the residue prior to the catalytic cysteine and ending after the conserved arginine) resulted in rmsd values of < 0.75 A ˚ (supplementary ... upon substrate binding, due to the presence of a small b-barrel domain that is unique to this protein and is involved in substrate binding [7,8]. No other resid...
Ngày tải lên: 18/02/2014, 18:20
Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
... mm atropine, and 10 )4 m without atropine in the presence of 1 mm carbamylcholine, which induced the maximum increase in GTPcS bind- ing (Fig. 3B), suggesting that carbamylcholine caused the decrease ... over the detection sensitivity in the western blot analysis and well transferred in the membrane fraction. Binding properties of muscarinic ligands with M 2 ::GOA-...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx
... the kinetic constants for hydrolysis and synthesis of penicillin G, ampicillin and cephalexin. Furthermore, insight into the nature of the transition state in the rate-determining steps of the ... leaving group, p-nitrophenol, occupies the acyl binding site and the acetyl group binds at the leaving group binding site, sterically hindering the deacylating H...
Ngày tải lên: 08/03/2014, 08:20