báo cáo khoa học: "Energie métabolisable et digestibilité azotée chez les poulets en croissance : (1) influence du génotype, de l’âge et du régime" ppt

... comparing the importin-a:CLIC4 NLS experimental electron density with density calculated from the model. The CLIC4 NLS main chain fits the density well, with an average RSCC of 0.96. Side chains have greater ... difference density peak near the Tyr205 O–C f bond at the 9.1r map level, the strongest density peak in the F clic4nls o ÀF apo o difference map. The presence of Tyr205 is de nitive e...

Ngày tải lên: 14/02/2014, 19:20

... colorectal cancer detection and diagnosis or as potential therapeutic targets. VDAC1, annexin A2 and Keratin 8 variant have been identified [20]. Madoz- Gurpide et al. tested seven potential markers ... protein identification confidence interval of > 95% were confi- dently assigned. When unique peptides were identified to multiple members of a protein family, proteins with the highest sequenc...

Ngày tải lên: 16/02/2014, 15:20

... zymogen is activated by a GRASP55-dependent furin-dependent mechanism, with the bulk of activation being GRASP55-independent. The interaction observed between GRASP55 and PC5/ 6B or PC7 together ... Protein concentration was determined using a BCA protein assay kit (Perbio Sci- ence UK Ltd, Cramlington, UK). The western blot presented is a representative of three independent experiments. Pro-...

Ngày tải lên: 18/02/2014, 04:20

... KLF4 is decreased in intestinal adeno- mas of multiple intestinal neoplasia mice and in colo- nic adenomas of familial adenomatous polyposis patients. In this investigation, we first determined ... against the devel- opment of atherosclerosis. By using the matinspector Professional program (http://www.genomatix .de) and the Transcription Element Search System (TESS; http://www.cbil.upenn.edu),...

Ngày tải lên: 18/02/2014, 04:20

... rearrange the pro-peptide. Generally, however, displacement of the pro-peptide cannot be excluded although in cova- lent peptide linkage, as the peptide region between the pro-peptide and the mature ... S1). Similarly, using proBMP-2 under identical assay conditions, only a low AP signal increase and no concentration dependence was recorded (Fig. 1B). Even when cells were stimulated with 1 lm...

Ngày tải lên: 18/02/2014, 06:20

... of Shh-N; when palmitoylated, the N-terminal portion would retain activity, even under denaturing condi- tions. To understand, in full, the 5E1 epitope under denaturing conditions, extensive analyses ... in the same blot. Values were the mean ± SD of three independent experiments. The difference between total Shh-Np and modified Shh-Np was determined using a paired t-test. *, P < 0.01; NS,...

Ngày tải lên: 18/02/2014, 16:20

... nicotinamide to move into a productive position [21]. This exchange between Y308 and the NADP + nicotinamide has been experimentally indicated as the enzyme rate-limiting step [18]. Evidence has recently ... arrange- ment of lumiflavin and phenol with the exact geometry found between flavin and Y308 in the crystal structure of pea FNR was used for the initial set-up. Then, dif- ferent arrangeme...

Ngày tải lên: 18/02/2014, 17:20

... Pseudomonas fluores- cens ACMSD revealed that the enzyme is metal- dependent and catalyzes a novel type of nonoxidative decarboxylation [21,33–35]. The human gene has been identified upon expression in ... quantita- ted as described in Experimental procedures, using the prim- ers ⁄ probe sets reported in Table 1. Data are the mean of three independent experiments and are presented as copies of...

Ngày tải lên: 19/02/2014, 02:20

... paralogue genes (e.g. promoters, enhancers) which lead to temperature- dependent mRNA levels have to be identified. Also, for a more detailed understanding of the functional and metabolic consequences, ... kJÆmol )1 [20,21] Pattern of heat denaturation (scanning rate ¼ 2 °CÆmin )1 between 10 and 110 °C) There are three independent transition states during denaturation: tetramer fi monomer fi dom...

Ngày tải lên: 19/02/2014, 02:20

... ROS dependent. Taking into account that NAC did not reduce the size of inclusion bodies, the difference in protection mediated by Hsps and NAC suggests a correlation between the ROS-dependent ... disease gene: evidence against simple inac- tivation due to an expanded CAG repeat. Somat Cell Mol Genet 20, 27–38. 6 Petersen A, Mani K & Brundin P (1999) Recent advances on the pathogenesis ......

Ngày tải lên: 19/02/2014, 06:20