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báo cáo khoa học: "Energie métabolisable et digestibilité azotée chez les poulets en croissance : (1) influence du génotype, de l’âge et du régime" ppt

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... comparing the importin-a:CLIC4 NLSexperimental electron density with density calculatedfrom the model. The CLIC4 NLS main chain fits thedensity well, with an average RSCC of 0.96. Sidechains have greater ... difference density peak near the Tyr205 O–Cfbond at the 9.1r map level, the strongest density peakin the Fclic4nlsoÀFapoodifference map. The presence ofTyr205 is de nitive evidence that ... per image. The crystal to detectordistance was set at 220 mm and the beam width was set to200 lm · 200 lm.Structure determination and refinementAfter datasets had been initially processed and...
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Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx

Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx

... colorectal cancer detection and diagnosis or aspotential therapeutic targets. VDAC1, annexin A2 andKeratin 8 variant have been identified [20]. Madoz-Gurpide et al. tested seven potential markers ... proteinidentification confidence interval of > 95% were confi-dently assigned. When unique peptides were identified tomultiple members of a protein family, proteins with thehighest sequence coverage ... iTRAQ-labeledpeptides with confident MS ⁄ MS identification (mascotscore ‡ 40), detected signature ions (m ⁄ z = 114, 115, 116,117), and performed automated quantitation of peptideabundance. For the detector...
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Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... zymogen is activated by aGRASP55-dependent furin-dependent mechanism, withthe bulk of activation being GRASP55-independent.The interaction observed between GRASP55 and PC5/6B or PC7 together ... Protein concentrationwas determined using a BCA protein assay kit (Perbio Sci-ence UK Ltd, Cramlington, UK). The western blot presentedis a representative of three independent experiments.Pro-MMP2 ... interactionobserved between the furin ICD and GRASP55 leadus to test whether both proteins could be detected inthe same membrane compartment. We were unable todetecte the endogenous level of furin...
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Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

... KLF4 is decreased in intestinal adeno-mas of multiple intestinal neoplasia mice and in colo-nic adenomas of familial adenomatous polyposispatients. In this investigation, we first determined ... against the devel-opment of atherosclerosis. By using the matinspectorProfessional program (http://www.genomatix .de) andthe Transcription Element Search System (TESS;http://www.cbil.upenn.edu), ... ICorrespondenceJ. Liu, Department of Histology andEmbryology, School of Basic MedicalSciences, Central South University,Changsha, Hunan 410013, ChinaFax: 86 731 82650400Tel: 86 731 82650436E-mail:...
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Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx

Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx

... rearrangethe pro-peptide. Generally, however, displacement ofthe pro-peptide cannot be excluded although in cova-lent peptide linkage, as the peptide region between thepro-peptide and the mature ... S1). Similarly, using proBMP-2 underidentical assay conditions, only a low AP signal increaseand no concentration dependence was recorded(Fig. 1B). Even when cells were stimulated with 1 lmproBMP-2, ... a standard deviation were deduced from five indepen-dent measurements. Apparent dissociation constants, KD,were calculated as KD= kd⁄ ka.The influence of the free pro-peptide on BMP-2...
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Tài liệu Báo cáo khoa học: Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog doc

Tài liệu Báo cáo khoa học: Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog doc

... ofShh-N; when palmitoylated, the N-terminal portionwould retain activity, even under denaturing condi-tions. To understand, in full, the 5E1 epitope underdenaturing conditions, extensive analyses ... in the same blot.Values were the mean ± SD of threeindependent experiments. The differencebetween total Shh-Np and modified Shh-Npwas determined using a paired t-test.*, P < 0.01; NS, not ... theABCFig. 3. Requirement of Cys25of Shh for Skn-dependent retentionof the 5E1 epitope on the N-terminal fragment of Shh in westernblotting. COS7 (A) and NSC34 (B) cells were transiently transfectedwith...
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Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx

Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx

... nicotinamide tomove into a productive position [21]. This exchangebetween Y308 and the NADP+nicotinamide has beenexperimentally indicated as the enzyme rate-limitingstep [18]. Evidence has recently ... arrange-ment of lumiflavin and phenol with the exact geometryfound between flavin and Y308 in the crystal structureof pea FNR was used for the initial set-up. Then, dif-ferent arrangements were generated ... region was detected from20° to 55°. Moreover, all differences in potential energyvalues obtained for arrangements at 4.6 A˚betweenangles from )20° to 85° were equal or lower than theenergy...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

... Pseudomonas fluores-cens ACMSD revealed that the enzyme is metal-dependent and catalyzes a novel type of nonoxidativedecarboxylation [21,33–35].The human gene has been identified upon expressionin ... quantita-ted as described in Experimental procedures, using the prim-ers ⁄ probe sets reported in Table 1. Data are the mean of threeindependent experiments and are presented as copies of the targetvariant ... (2006) Stimulation ofnicotinamide adenine dinucleotide biosynthetic path-ways delays axonal degeneration after axotomy. J Neuro-science 26, 8484–8491.45 Cooper BF, Sideraki V, Wilson DK, Dominguez...
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Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt

... paralogue genes(e.g. promoters, enhancers) which lead to temperature-dependent mRNA levels have to be identified. Also,for a more detailed understanding of the functionaland metabolic consequences, ... kJÆmol)1[20,21]Pattern of heat denaturation (scanningrate ¼ 2 °CÆmin)1between 10 and 110 °C)There are three independent transition states during denaturation: tetramer fimonomer fi domain 1 ... protein kinase CK2 [14,15], F0F1-ATPase[16], etc. Temperature-dependent gene expression ofthese enzymes may result from: (a) the temperature-dependent expression profile of transcription factorslike...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt

Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt

... ROS dependent. Taking into account that NAC didnot reduce the size of inclusion bodies, the differencein protection mediated by Hsps and NAC suggests acorrelation between the ROS-dependent ... disease gene: evidence against simple inac-tivation due to an expanded CAG repeat. Somat CellMol Genet 20, 27–38.6 Petersen A, Mani K & Brundin P (1999) Recentadvances on the pathogenesis ... fluorescence was monitored by FACS cytometry as describedin Experimental procedures. The fluorescence index wasdetermined as the ratio of the fluorescence of cells expressinghttEx1-polyQ polypeptides...
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