Báo cáo y học: "Living the high life: high-altitude adaptation" pptx

... respectively. Interestingly, there is no overlap in candidate regions identified in the two populations. When the authors restrict their analysis to candidate genes and pathways involved in high- altitude ... EPAS1, on the other hand, was only identified in the Tibetan populations, and PPARA was not considered with the HIF pathway genes in the Andean analysis, so it remains...

Ngày tải lên: 09/08/2014, 20:22

... interact with the tyrosine motifs pY401 and pY429pY431. pY429pY431 was more potently recruiting SOCS-3 than pY401, reflecting the high affinity binding determined by SPR. The nonphosphorylated peptide Y4 2 9Y4 31 ... SOCS-3. Peptide K d (l M ) pY343 > 30 pY401 9.5 ± 0.12 pY429pY431 1.1 ± 0.03 F429pY431 4.6 ± 0.02 pY429F431 4.9 ± 0.02 Y4 2 9Y4 31 ND pY443 ND pY461pY464 ND Y4 61pY46...

Ngày tải lên: 24/03/2014, 00:21

... anticipated. These factors significantly reduced the power of the study. Based on their findings, the authors estimate that more than 100 hospitals would be needed to show the 30% difference they sought. ... seen from baseline to the study period for both groups combined. Conclusion The MET system greatly increases emergency team calling, but does not substantially affect...

Ngày tải lên: 25/10/2012, 10:39

... series will probably be familiar, it is hoped that there will still be useful lessons to be learned, for example the underlying assumptions of a hypothesis test that were not fully appreciated, or ... some previously unrecognised confusion between terms. The first article, presented in this issue, covers the presentation and summary of data. It is unlikely that the material covered by...

Ngày tải lên: 25/10/2012, 10:45

... the catalytic efficiency of the enzyme, the cold adaptation of the enzyme could not be attributed to the presence of the Gly cluster in this element. The present study supports the idea that the ... Gly261 by Ala in mutant G261A resulted in an enzyme with slightly lower stability. On the other hand, in the double mutant G261A /Y2 69A the additional replacement of Tyr269...

Ngày tải lên: 22/02/2014, 04:20

... guanidine hydro- chloride (GdnHCl), urea or elevated temperatures, they destroy the tertiary structure of proteins which results in the loss of enzymatic activity. Regarding the secondary struc- ture ... detected by proteolysis (see below), we investigated RNase A in the absence and presence of 20% trifluoroethanol by NOESY and TOCSY NMR spectroscopy. However, due to the high pH va...

Ngày tải lên: 22/02/2014, 07:20

... angle almost typical for the 3 10 helix. In this region of the polypeptide chain, the secondary structure is characterized also by the O i H N i þ 3 hydrogen bonds, found in all 40 DYANA structures ... residue for the final family is given in Fig. S1. Fig. 3A shows the family of 40 structures and Table 1 contains a summary of statistical data indica- ting the quality of the 40...

Ngày tải lên: 17/03/2014, 10:20

... catalytic activity proceed essentially by the same mechanism. Indeed, catalytic residues occupy the same position in the 3D structures of the two enzymes. In addition, the p redicted secondary structures ... function, display only one polar cavity in the vicinity of the conserved catalytic Asp re sidue. Keywords: neurospora; P-ATPase; homology model; cavity; H + . The 3D struc...

Ngày tải lên: 23/03/2014, 21:20

... previously described [9,15]. MALDI-TOF/MS was performed on a Perseptive DE-RP (Applied Biosystem) using a-cyano-4-hydroxycin- namic acid as matrix. Lethality assay in mice The in vivo toxicity of ... venoms and become key molecular probes for the characterization of these channels. They are usually small basic polypeptides (between 30 and 70 amino acids), cross-linked by three or four dis...

Ngày tải lên: 23/03/2014, 21:20

... Results from other coprecipitation studies supported the yeast two-hybrid results with the exception of the weak interaction observed between LEF-3 and IE1 with the yeast two-hybrid system (Table ... of PCR products into the BglII and PspAI sites of plasmid phsp70HAHisVI+ containing the 5¢ terminal amino acid sequence RYPYDVPDYARA. The sequence YPYDVPDYA constitutes the haemag...

Ngày tải lên: 23/03/2014, 21:20