Báo cáo y học: "Community-wide analysis of microbial genome sequence signatures" pptx

... approximately 210 Mb of total sequence, which assembled into 15,929 contigs and 36.6 Mb of compos- ite sequence. Phylogenetic analysis The phylogenetic tree of 16S rRNA genes was constructed by neighbor ... inherent diversity of microbial communities typically limits genomic assembly, resulting in highly fragmentary data [13]; there are few uni- versally conserved phylogenetic...

Ngày tải lên: 09/08/2014, 20:20

... to identify key molecules in the course of hy- poxia-induced apoptotic pathways. Such analysis of trophoblastic cells will, in the near future, yield in- sights into the mechanisms of preeclampsia ... biology and biochemistry have enabled analy- sis of the expression profiles of numerous proteins at once. In this study, we looked at hypoxia-induced apoptosis and, by using the...

Ngày tải lên: 31/10/2012, 15:12

... Big Dye Terminator system (Applied Biosystems, Germany). Sequences were analyzed using the Chromas software (Technely- sium Pty Ltd, Tewantin, Australia) and GeneDoc software (http://www.psc.edu/biomed/genedoc ). ... hCDC4 protein. Sequence analysis of hCDC4 exon 10 identifies a new intronic SNP During the analysis of exon 10 and adjacent intronic sequences we could for the fi...

Ngày tải lên: 31/10/2012, 16:49

... levels of many of these genes identified in the array analysis. Moreover, Western blot analysis of thymo- cyte lysates also confirmed that IgM increases with age (Figure 3). Flow cytometry analysis ... and array analysis. For each array sample, the RNA was prepared from the purified thymocytes. The thymocytes were processed by using the RNEasy Mini Kit (Qiagen, Valencia, CA)....

Ngày tải lên: 03/11/2012, 11:35

... potentiated by enzymatic degradation of phospholipids [7,44]. The capacity of DM64 to inhibit the myotoxicity induced by myotoxins I (Asp49) and II (Lys49) from B. asper venom was analyzed in vivo ... three domains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64. Cytotoxicity was analyzed in vitro using C2C12 sk...

Ngày tải lên: 21/02/2014, 01:21

... H.S., Han, Y. & Fung, B.K K. (2000) Subunit e xchange of small h eat sho ck proteins. Analysis of olig- omer formation of aA-crystallin and Hsp27 by fluorescence res- onance energy transfer ... chaperoning in vitro [66], and disassembly of active units from an oligomeric (storage) state of a-crystallin was proposed, upon structural analysis of aA-crystallin by site- direct...

Ngày tải lên: 22/02/2014, 04:20

... formation of gamma glutamyl-lysine bonds [4]. Furthermore, tTG plays a role in the transduction of extracellular signals, mediated by its additional GTP- hydrolyzing activity [5], analogous to that of ... member of a family of seven isoforms of enzymes involved in protein cross-linking, including prostatic TGase and factor XIII. tTG is a Ca 2+ -dependent ubiquitous intracellular e...

Ngày tải lên: 08/03/2014, 09:20

... 2-N-(3-O- acyl 4 -acyl 2 )-b-GlcN-(1–6)-2-N-acyl 1 )3-O-acyl 3 -GlcN where acyl 1 ,acyl 2 and acyl 3 are 3-hydroxyhexadecanoic or 3-hy- droxyoctadecanoic acids, acyl 4 is tetradecanoic or (minor) hexadecanoic ... Acyl 1 ,acyl 2 and acyl 3 residues had hydroxy or acyloxy groups at C-3 ( 13 C signals of C-3 at 69.0–72.4 p.p.m.), while acyl 4 had no substituents. The signals of acyl chains c...

Ngày tải lên: 08/03/2014, 09:20

... chemical analysis of 7-O-(2-amino-2-deoxy-a- D -glucopyranosyl- L -glycero- D -manno-heptose as a constituent of the lipopolysaccharides of the UDP-galactose epimerase less mutant J-5 of Escherichia ... of carbon. Additionally, long-range correlations between protons of adjacent sugar residues across the glycosidic bond established their sequence; this was confirmed by the anal...

Ngày tải lên: 08/03/2014, 09:20

... 2002 Kinetic analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by an Escherichia coli expression system 7 Tatsuya 7 Kitazume 1 , Akinori Tanaka 1 , Naoki Takaya 1 , ... fundamentally as described previously [15] by gas chromatography (GC) and GC-electron impact-mass spectrometry (GC-EIMS). Prior to analysis, the carboxyl group of the products was blo...

Ngày tải lên: 08/03/2014, 22:20