Tracking developmental changes in gene expression pot

... both. Using expression maps to generate hypotheses The current dataset of gene expression in the root [5] provides a rich resource for those interested in plant development. Cell- type-specific expression ... could be observed by fitting all differentially expressed genes to a hypothetical gene- induction curve; and the similarities between expression profiles for all genes i...

Ngày tải lên: 09/08/2014, 20:20

... treatment. Changes in Global Gene Expression Gene expression in PBMC changed dramatically and rap- idly during PEG-interferon-α2b (PegIntron™)/ribavirin therapy, with major changes being evident ... [2,22-25,32]. Among gene functions significantly altered by IFN are genes involved in the immune response including inflammation, genes previ- ously reported to be involved in...

Ngày tải lên: 18/06/2014, 15:20

... shown that TTR expression decreases significantly in a cytokine-induced APR model [17], and that changes in HNF-4a and HNF-1a binding can be seen very rapidly in a murine burn injury model [16,18]. ... reduction in the binding of HNF-4a, HNF-1a, HNF-3b and HNF-6a to the transthyretin promoter, which cor- relates with a decrease in transthyretin expression after injury. These fin...

Ngày tải lên: 23/03/2014, 03:20

... that includes a DNA-binding domain, a ligand-binding domain and a transactivation domain. The DNA-bind- ing domain is responsible for DNA binding specificity and dimerization, and the ligand-binding ... domain involved in protein–protein interactions. FYRC and FYRN domains are involved in heterodimerization between MLL N and MLL C terminal fragments. High-mobility group (HMG) domains are i...

Ngày tải lên: 16/02/2014, 14:20

... (v/v) heat inactivated fetal bovine serum (Gibco) and 100 lgÆmL )1 penicillin/streptomycin. C2C12 cells were cultured as mono- layers in Falcon tissue culture dishes in a medium containing 10% (v/v) ... described in Materials and methods using 30 lg mitochondrial protein in each case. Ó FEBS 2003 Cytochrome c oxidase subunit expression in hypoxia (Eur. J. Biochem. 270) 875 transcr...

Ngày tải lên: 20/02/2014, 23:20

... thereby eliminating the 3¢-heter- ogeneity inherent in conventional oligo(dT) priming [14]. As the PrimeScript Reverse Transcriptase exhib- ited terminal transferase activity upon reaching the end ... SAGE tags originating from potentially novel transcripts or novel genes are unidentified in the human genome. We have constructed a SAGE library on human spermatozoa in which we obtained mo...

Ngày tải lên: 07/03/2014, 04:20

... regulation of SHP-1 expression. To confirm whether USF proteins bind to the 12 bp repeat in the (I)SHP-1 promoter, in vivo binding of USF proteins can be assessed by formaldehyde cross-linking followed ... the proteins involved in these complexes have very low ÔonÕ rates, resulting in an ineffi- cient, albeit stable binding to the oligonucleotides. Our finding that an oligonucleotide be...

Ngày tải lên: 08/03/2014, 23:20

... fibrinogen- domain containing proteins, fibrinogens, fibroleukins/ techylectins, angiopoietins, ficolins and tenascins, the following consensus was deduced: FSTxDNDND. It is located in the human fibrinogen ... protein was identified biochem- ically. The binding of the linear carbohydrate, curdlan, was abolished by the branched molecule, laminarin, indicating that in S. domuncula a binding prote...

Ngày tải lên: 16/03/2014, 16:20

... during certain developmental stages. The observation that Drosophila dmeglob1 pro- tein exhibits ligand-binding properties and expression patterns that resemble those of other known insect globins ... ROS-generating chemicals revealed a complex gene regulatory response, including the expected upreg- ulation of antioxidant defense genes [17,18]. Many invertebrates harbor respiratory prot...

Ngày tải lên: 30/03/2014, 02:20