Transcription-coupled mutagenesis by the DNA deaminase AID Erik D Larson and Nancy Maizels ppt

... research Minireview Transcription-coupled mutagenesis by the DNA deaminase AID Erik D Larson and Nancy Maizels Address: Departments of Immunology and Biochemistry, University of Washington Medical School, 1959 NE ... Honjo, Durandy and collaborators showed that a single polypeptide, activation- induced deaminase (AID) , induces both switch recombina- tion and...

Ngày tải lên: 09/08/2014, 20:20

... on days 0, 3 and 6, where day 0 begins on the day of randomization. Radiation (4 Gy) was delivered 24 h after the first injection of BO-1051 (day 1 after randomization), which corresponded to the ... of the repair to the DNA double-strand breaks (DBSs). Materials and Methods Cell lines and treatment This research followed the tenets of the Declaration of Helsinki. All sa...

Ngày tải lên: 09/08/2014, 09:20

... methods. Firstly, the author used analytic and synthetic methods to review the theories related to the matter concerned to form a framework for the analysis, and then collected books and materials ... find and select necessary information. Secondly, the data collected from the survey were analyzed and sorted to make a clear distinction about the students’ attitudes tow...

Ngày tải lên: 18/12/2013, 10:08

... sustained (> 3 h) and independent of EGFR but dependent on Src and Shc. Interestingly, RNAi-mediated down-regulation of E-cadherin reduced Decma-induced Erk activation (Fig. 2D) but did not ... p52 Shc3Y3F . These results revealed a nonredundant and isoform-specific role for Shc in the Erk signaling induced by the E-cadherin blockage. Decma-induced Shc tyrosine phosphorylation...

Ngày tải lên: 19/02/2014, 05:20

... encoded residues 1–505 and 653–725 of TAP1 and residues 494–639 of TAP2 and chimera 2C1 encoded residues 1–493 and 640–703 of TAP2 and residues 506–652 of TAP1. The exchange was performed at the ... distinct nucleotide binding and accordingly the different function- ality of NBD1 and NBD2, we generated TAP1 and TAP2 chimeras in which either the nonhomologous C-terminal...

Ngày tải lên: 20/02/2014, 02:21

... used: (i) 1 : 20 and (ii) 1 : 100. Digests were run at 26 °Cfor the times indicated and then separated by SDS ⁄ PAGE and the gel was Coomassie stained. Two major degradation products of WT- and YQ-proCPU-CHis ... separated by SDS ⁄ PAGE and transferred to a polyvinylid- ene difluoride membrane for N-terminal sequencing (Amidoblack staining). The bands indicated by numbers...

Ngày tải lên: 07/03/2014, 12:20

... MgCl 2 concentration dependence experiments on GUV lysis induced by peptides were conducted and it was found that the electrostatic interactions between peptides and lipids are little influenced by the addition ... [1-ethyl-3-(3-dimethylaminopro- pyl)carbodiimide] and NHS (succinimide), and the activated esters that had not bound ligand were capped with ethanolamine. The ma...

Ngày tải lên: 08/03/2014, 08:20

... expres- sion. CD40-ligand (CD40L), also known as CD154, is the chief ligand for CD40 and is expressed primarily by acti- vated T cells and platelets [10,11]. The interaction of CD40 and CD40L represents ... evaluated by flow cytometry using a FACSCanto cytometer and FACSDiva software (BD Biosciences) and the following mouse anti- human mAb: CD40 (AbD Serotec, Raleigh, NC);...

Ngày tải lên: 18/06/2014, 15:20

... bubble-migration synthesis, lagging strand synthesis does not occur, and the newly synthesized single strand is extruded from the back of the D- loop as new DNA is synthesized at the front of the D- loop (panel ... created and then quickly extruded from its template, and lagging-strand synthesis does not occur within the D- loop. In the RDR reactions analyzed by For...

Ngày tải lên: 11/08/2014, 21:21

... helicase loading protein, the gp61 primase, and the gp32 single stranded DNA binding protein. A.) the gp32 single-stranded DNA binding protein binds to regions of displaced DNA near the replication ... the gene 45 sliding clamp (gp45), the gene 44 and 62 encoded ATP-dependent clamp loader complex (gp44/62), and the gene 32 encoded single-stranded DNA binding protei...

Ngày tải lên: 11/08/2014, 21:21