Báo cáo sinh học: " Chromosomal localization and activity of nucleolar organizer regions in the dog" pot

... of the canine genomepossible. The objective of the present study was the chromosomal localization of NORs in the dog karyotype and the analysis of their activity. This ... were localized in the terminal part of the q arms of chromosome pairs: 7 and 17. The third chromosomepair, also bearing NORs in the terminal part of the q arm, belongs ... 1. INTRODUCTION In the nucleolar organizer regions (NORs), there are genes for 5.8S, 18S and 28Sribosomal RNA. These regions can be visualized by the silver staining...

Báo cáo sinh học: " Expression pattern and polymorphism of three microsatellite markers in the porcine CA3 gene" pptx

... from the beginning of intron 4 and the novel microsatellite, which included a tandem repeat of (TC)nis located approximately at 1264 bp from the beginning of intron 4.3.3. Allele frequencies in ... protein characteristic of adulttype-1, slow-twitch, muscle ﬁbres. In order to further understand the functions of the porcineCA3 protein in muscle, the temporal and spatial distributions of its ... Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture,Huazhong Agriculture University, Wuhan, 430070, P. R. China(Received...

Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx

... biological endpoints in decidingwhen to administer intervention, monitoring the effec-tiveness of any current intervention, determining the effi-cacy of drugs, assessing drug resistance and are usefulresearch ... attending a GUM clinic in The Gambia. The possible role of other co-infections in the secretion of HSV in CVL fluid and HSV transmission wereinvestigated.ResultsStudy subjectsSeventy subjects included ... problemsrelating to sensitivity of the HSV-1 and -2 probe relativebinding. In a single step multiplex assay mutations in the probe binding area are reported to lead to a loss in sensi-tivity and error in...

Báo cáo sinh học: "Bending out and breaking away: host-cell accomplices in retroviral escape" pot

... machinery that constricts the neck of the bud, resulting in fusion between the membraneson either side of the neck and the release of the virus from the plasma membrane. Studies with a number of ... overcoming the mechanical bending resistance of the plasma membrane [2]. Third, following the formation of the bud, the virus has to pinch off and escape from the cell(Figure 1d,e) [3]. This involves ... incorporated into the virions. Inter-estingly, ␣-adaptin and clathrin, two other components of the clathrin-mediated endocytic machinery [28], were alsofound to be incorporated into MuLV virions. The...

Báo cáo sinh học: "Comprehensive curation and analysis of global interaction networks in Saccharomyces cerevisiae" doc

... Toreveal potential bias in the extent of investigation of anygiven node in the LC datasets, we determined the number of total interactions (that is, including redundant inter-actions) in excess of ... for interrogation of gene and protein function, including DNA microarrays for globalgene-expression profiling and location of DNA-bindingfactors, and a comprehensive set of gene deletion strains ... [37], the Molecular Interactions (MINT) database [38], the IntActdatabase [39], the Database of Interacting Proteins (DIP)[40], the Biomolecular Interaction Network Database(BIND) [41], the...

Báo cáo y học: "Gene expression and activity of cartilage degrading glycosidases in human rheumatoid arthritis and osteoarthritis synovial fibroblast" potx

... degradationmay dominate and lead to the release of glycosaminoglycansfrom the extracellular matrix.One of the most striking findings of our study was that the reg-ulation of gene expression of glycosidases ... detected in the homoge-nates of either the SMs or the SFs (Figure 3a to 3c). The activ-ity of NAG in RA SFs was significantly higher than in RA SMs. In contrast, the activity of GusB in SFs was ... and activity in the joints. This is in accordance with the findings of previous studies [29]. In the present study we show that SFs appear to be majorsources of this enzyme in the SMs as they...

Báo cáo sinh học: "Evidence for large domains of similarly expressed genes in the Drosophila genome" docx

... the window size increases from two to eight genes, the netnumber of genes in groups (that is, the genes in groups in the ordered dataset minus genes in groups from the random dataset) increases ... genes in a sliding window across the genome, and this process was repeated for windows of different sizes. The netnumber of genes (that is, the number of genes in groups in the ordereddataset minus ... aligned to the chromosomearm, in Figure 2. The distribution of groups along the chro-mosomes appears random and there is little bias for genes in a group to be on the same strand. The length of genomicsequence...

Báo cáo y học: "Topical anti-inflammatory activity of Polygonum cuspidatum extract in the TPA model of mouse ear inflammation" docx

... dryweight of PCE. Most of the solids in the commercialextract were not soluble in 50% ethanol. The dry weight of the ethanol-insoluble pellet remaining after centrifuga-tion of the extract ... the topical application of 2 µg TPA dissolved in 20 µL of ace-tone to both the inner and outer ear surfaces. Thirty min-utes after the application of TPA, the inner and outersurface of each ear was ... applied to the skin and in nutraceutical products that are ingested, itis worthwhile to test PCE activity in this model. The skin and gastrointestinal mucosa are both subject to inflam-mation,...

Báo cáo khoa học: Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor pptx

... our understanding of the mechanisms underlying some of these characteristics, wehave explored the effects of combining activating and inactivating mutations of the TSHr within a single receptoron ... response and (f) the mechanism by which binding of the hormone to the N-terminal domain of the receptor translates into activation of its serpentine domain is unknown.With the aim of improving our ... of diseases [31]. In conclusion, the combination of two activating muta-tions of the TSHr determined an increase in the activity onlyfor the cAMP pathway and not for the IP pathwaysuggesting...

báo cáo sinh học:" Appropriate training and retention of community doctors in rural areas: a case study from Mali" pdf

... implicationsAs the training consists to a large extent in compensatingfor shortcomings of the medical school-based training, areasonable approach would be to incorporate this kind of training in the basic ... responses and the final evaluationsession indicated a high level of satisfaction regarding the training. Though they expressed a high demand for fur-ther training, in particular in clinical issues, ... from other incentives and support mechanisms (follow upvisits, continuing training, mentoring ) affecting job satisfaction.Conclusion: Training increasing self confidence and self esteem of rural...

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