Báo cáo y học: "Phenotypic and functional characterization of switch memory B cells from patients with oligoarticular juvenile idiopathic arthritis" pps

... expansion of < /b> switch < /b> memory < /b> B cells and < /b> IgG-secreting plasma blasts in the SF from oligoarticular JIA patients. These memory < /b> B cells belonged to both the CD27 + and < /b> the CD27 - subsets and < /b> expressed ... switch < /b> memory < /b> B cells from juvenile idiopathic arthritis (JIA) patientsChemokine receptor and < /b> CD86...

Ngày tải lên: 09/08/2014, 14:22

... expressed by several cell types (lym- phocytes, dendritic cells, B cells and < /b> plasma cells) [18,24], only areas characterised by a clear lymphocyte infiltration (as defined by anti-CD3 and < /b> anti-CD4 ... intensity. (a, b) Expression of < /b> IFN-γ (a) and < /b> CXCR3 (b) by SF CCR7 + and < /b> CCR7 - memory < /b> CD4 + cells from 10 patients with juvenile i...

Ngày tải lên: 09/08/2014, 06:22

... digestions of < /b> deglycosylated and < /b> untreated smelt AFP in presence and < /b> absence of < /b> Ca 2+ . Both deglycosylated and < /b> untreated smelt AFP were incubated with protease in the presence or absence of < /b> 20 m M Ca 2+ as ... Modulation of < /b> ligand-binding activity by Ca 2+ and < /b> pH. J. Biol. Chem. 263, 9752–9760. 28. Varki, A. (1993) Biological roles...

Ngày tải lên: 24/03/2014, 03:21

... performed with an anti-AK polyclonal antibody obtained from previously immunized rabbits. A single band of < /b> 26 kDa was detected, as shown in Fig. 4B. Functional < /b> characterization < /b> of < /b> recombinant AK The ... homogeneity. Kinetic analysis of < /b> purified L. donovani AK showed hyperbolic behaviour for both ATP and < /b> AMP, with K m values of < /b> 104 and <...

Ngày tải lên: 21/02/2014, 00:20

... agrees with the large body of < /b> independent data collected on Calb and < /b> truncated Calbs by Kumar’s group. This latter work revealed that EF-hands II and < /b> VI of < /b> Calb do not bind calcium and < /b> that Calb binds ... Poland; 2 Department of < /b> Biochemistry and < /b> Molecular Biology, University of < /b> Debrecen, Hungary; 3 Department of < /b> Inorganic Chemi...

Ngày tải lên: 22/02/2014, 07:20

... cotransformed by constructs containing CRE, CaRe, AP1, NF-jB and < /b> SRE elements and < /b> different CRFR1 isoforms. (A) Stimulation by CRF. (B) Stimulation by CRF, forskolin and < /b> PMA (TPA) and < /b> inhibition by PKA ... 5% fetal bovine serum, antibiotics and < /b> 0.5 m M 3-isobutyl-1-methylxanthine (IBMX), and < /b> transfected COS cells were incubated with the ligand for...

Ngày tải lên: 07/03/2014, 15:20

... using the yeast two-hybrid system as previously described [9]. A 915-bp fragment was initially identified and < /b> further confirmed through both repetitions of < /b> the yeast two-hybrid assay and < /b> using ... that VBARP treatment is specific and < /b> does not alter the global cellular transcription. Effect of < /b> VBARP siRNA on cell viability was tested by the trypan blue exclusio...

Ngày tải lên: 07/03/2014, 21:20

... characteristic of < /b> the S100 family. Metal-binding properties were studied by a direct 45 Ca 2+ -binding assay and < /b> by fluorescence titration. Calhepatin binds Ca 2+ and < /b> Cu 2+ but not Zn 2+ .Cu 2+ binding ... with a letter (E for peptides derived by Glu-C digestion and < /b> K for peptides obtained by Lys-C digestion) and < /b> a number that agrees with that of < /b>...

Ngày tải lên: 08/03/2014, 22:20

... Germany 4 Macromolecular Crystallography, BESSY GmbH, Berlin, Germany 5 Institute of < /b> Chemistry and < /b> Biochemistry – Crystallography, Free University, Berlin, Germany 6 Department of < /b> Structural Biology, ... morphology of < /b> the generated filament bundles. Calcium affinity of < /b> Iba1 and < /b> Iba2 Homodimerization and < /b> actin binding of < /b> Iba1 and < /b> Iba...

Ngày tải lên: 16/03/2014, 06:20

... on black background, and < /b> conserved amino acid residues with high and < /b> low similarity are indi- cated by double dots and < /b> single dots, respectively. Amino acid residues plausibly involved in the binding ... report demonstrates broad substrate specificity, with d-Glu and < /b> d-Ala being high-affinity substrates. The various d-amino acids found in most plants [37] may be...

Ngày tải lên: 30/03/2014, 04:20