Báo cáo khoa học: " Isobologram analysis of triple therapies" pdf

... equations. ab c d BioMed Central Page 1 of 8 (page number not for citation purposes) Radiation Oncology Open Access Methodology Isobologram analysis of triple therapies Maximilian Niyazi* and ... level of interaction of two agents. In this regard, isobologram analysis was developed and described 30 years ago and is still the most popular tool for this question [1,8]. Basical...

Ngày tải lên: 09/08/2014, 10:21

... Department of Structural and Functional Biology, and Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy 2 Department of Biotechnology, University of Verona, Italy 3 Department of Clinical ... specifically linked to one of the major patho- genetic mechanisms of PD [5]. Worthy of note is the complete disappearance of the VDAC-2 upon dopa- mine treatment. Thi...

Ngày tải lên: 15/02/2014, 01:20

... re-plot of Dslope )1 of the Lineweaver– Burk plot as a function of the reciprocal of the benzalkonium concentration. (B) Mutant D70G:d, no benzalkonium; s,2l M; , 3 l M; h,4lM. Modulation of butyrylcholinesterase ... hydrolysis of aryl acylamides by BuChE [33]. However, several lines of structural evi- dence clearly rule out this hypothesis. First, kinetic analysis of organo...

Ngày tải lên: 18/02/2014, 17:20

... thylakoids in the absence of additives or in the presence of 1 lm DCMU or dPQ over a range of temperature from 15 °Cto25°C. The range of tem- perature was set on the basis of the membrane trans- ition ... superposition of the exponential rise to analyze quantitatively the contribution of each phase. The amplitude and rate constant of each of the three phases can be calculate...

Ngày tải lên: 19/02/2014, 05:20

... (in subunits). Inset: plo t of 1/DA as a function of the reciprocal total pro tein concen tration. T he reciproca l of the Y-in ter- cept of this plot gave th e value of DA 1 , the change in a ... excess of CuTCPP and C uTMPyP are also shown. A fi t of the CD spectrum of native MCL, obtained by analysing the spectrum using the CDSSTR program, is also given (details of the spe...

Ngày tải lên: 19/02/2014, 16:20

... viscosity of t he extract as a result of D NA, 2 lLof DNase I (200 units) was added per 100 lL of buffer [30]. Following filtration through two layers of miracloth a nd the addition of four volumes of ... ÔStains- AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4. As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional...

Ngày tải lên: 19/02/2014, 16:20

... S DS/PAGE with 10% of input 35 S-labelled DEC1, and visualized by autoradiography. Ó FEBS 2004 Interaction of DEC1 with BMAL1 (Eur. J. Biochem. 271) 4417 Functional analysis of the basic helix-loop-helix ... Kato 1 1 Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan; 2 Department of Physiology, Hokkaido U...

Ngày tải lên: 19/02/2014, 16:20

... Formation of the covalent serpin– protease complex involves a Michaelis docking complex, cleavage of the P 1 –P 1 ¢ peptide bond, linkage of the active site Ser of the protease to the carboxyl group of ... Mechan- isms of conversion of plasminogen activator inhibitor 1 from a suicide inhibitor to a substrate by monoclonal antibodies. J. Biol. Chem. 277, 43858–43865. Ó FEBS 2003...

Ngày tải lên: 20/02/2014, 11:20

... conformational properties of HpPex5p, the PTS1 receptor of the yeast H. polymorpha. Fluorescence emission spectra revealed a pH dependence of Trp fluorescence of HpPex5p, indicative of pH driven changes ... concluded from the structural model of the PTS1-binding C-terminal domain of HpPex5p (Fig. 4). In this domain three of the seven Trp residues of HpPex5p are found. However...

Ngày tải lên: 21/02/2014, 00:20

... Thermodynamic analysis of Jun–Fos coiled coil peptide antagonists Inferences for optimization of enthalpic binding forces Jonathan A. R. Worrall and Jody M. Mason Department of Biological Sciences, ... energy of bind- ing. Indeed, the free energy of binding is 2–3 kcalÆmol )1 less than any of the antagonist–cJun or antagonist– cFos complexes. ITC data collected from the leucine...

Ngày tải lên: 06/03/2014, 01:20