Báo cáo y học: "Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration" ppt

... participated in the design of the study, performed the majority of the laboratory work and analysis, and drafted the manuscript. AJF participated in the design of the study and interpretation of data. JAH ... CCT ACC ACG AAA GCA GAT C 3' 5' 6 FAM – CCC AGG ACA GAC CTA CGA TGC CAC C – TAMRA 3' 5' GCC GGG ACA CAC GGA GTA 3' 99.74% ADAMTS 5,...

Ngày tải lên: 09/08/2014, 10:20

... Analyses of BALF chemokine and cytokine levels can probably provide therapeutic targets in SSc-associated ILD. Competing interests The authors declare that they have no competing interests. Authors' ... allowing the detection of cytokines without any BALF manipulation that could influence the stability of cytokines. By using this technique, we observed abnormalities in a...

Ngày tải lên: 09/08/2014, 14:22

... 789 Conformationally constrained human calcitonin (hCt) analogues reveal a critical role of sequence 17–21 for the oligomerization state and bioactivity of hCt Athanasios Kazantzis 1 , Michaela Waldner 1 , ... with a Kratos Compact MALDI I (Shimadzu Europe, D uisburg, Germany) and a- cyano-4-hydroxycinnamic acid as matrix. Purity of the HPLC purified peptides were also confirme...

Ngày tải lên: 31/03/2014, 21:21

... PCR Human IL-32 Sense 5'-TGAGGAGCAGCACCCAGAGC-3' Antisense 5'-CCGTAGGACTGGAAAGAGGA-3' Human TNFα Sense 5'-GTCTCCTACCAGACCAAG-3' Antisense 5'-CAAAGTAGACCTGCCCAGACTC-3' Human ... 5'-TGGGAGTAGACAAGGTACAACCC-3' Mouse IL-1β Sense 5'-CAACCAACAAGTGATATTCTCCATG-3' Antisense 5'-GATCCACACTCTCCAGCTGCA-3' Mouse IL-6 Sense 5'-CACTT...

Ngày tải lên: 09/08/2014, 08:23

... collected at the time of joint harvest at pre-CIA (days 16, 22, and 28 after immunization), early-CIA (within 2 days of receiving a score of 3 for any joint), and chronic-CIA (80 days), and teased into ... University and later at the University of Michigan. Animal care at the Unit for Laboratory Animal Medicine is supervised by a veterinarian and operates in accordance wit...

Ngày tải lên: 09/08/2014, 06:23

... messenger RNA (mRNA) have been found in the cytoplasm of neurons and in thenucleiofastrocytesof many brain regions [5-8]. FGF-2 plays a role in the neu- ronal development in prenatal life and also influence survival ... protocols approved by the Animal Care and Use of Ethic Committee at the University of São Paulo and in accordance with the Guide for Care and Use...

Ngày tải lên: 10/08/2014, 10:20

... these organelles, where they s erve as the substrates for the elongation of carbohydrate chains by appropriate glyco- syltransf erase s. Changes in the activities of NSTs may affect the structure of ... structure of oligosaccharide chains by affecting the availability of substrates for glycosyltransferases [1]. In fact, in organisms such as Drosophila melanogaster an...

Ngày tải lên: 21/02/2014, 15:20

... immunostaining was observed mainly in epithelial cells of the secondary lamella (Fig. 8, panels a and e). The staining pattern was reminis- centofthatoffreshwater-typechloridecellsthathave recently ... remode- ling of the gill architecture and its maintenance by targeting, for degradation via the proteasomal pathway, a group of regulatory and structural proteins that are n...

Ngày tải lên: 17/03/2014, 10:20

... 2. 5 Quality of the electron crystallography data. (a) Cryo-electron crystallography data after averaging over 21 separate untilted crystalline areas. The size of the box and n umber indicate the ... account the redundancy of the data and hence can g ive a misleading pessimistic impression of the reliablility of oversampled data. The three-dimensional data set was...

Ngày tải lên: 17/03/2014, 17:20

... a catalytically inactive DAP kinase mutant and a construct containing the death domain and C-terminal tail of DAP kinase, which act in a dominant-n egative manner, rescued cells from C 2 -, and ... ratio was increased. These results suggest that CaM kinase IV is activated through a CaM kinase kinase cascade triggered by a n increase in intracellular Ca 2+ levels, and as CaM...

Ngày tải lên: 24/03/2014, 00:21