oxidative stress in the pathogenesis of diabetic nephropathy

... stabilization of HIF [39] Our results of catabolic stress- induced expression of HIF-1α in chondrocytes are consistent with these findings These findings suggest that HIF-1α may, at least in part, ... maintain chondrocyte viability in the face of catabolic changes in articular cartilage Many molecular aspects of HIF-1 signaling should be also studied to further clarify the role of HIF-1 in the ... Germany) was used The assay is based on the light-emitting oxidation of luciferin by luciferase in the presence of extremely low levels of ATP After collecting the chondrocytes by scraping, cells were...

Ngày tải lên: 09/08/2014, 06:23

Ngày tải lên: 09/08/2014, 07:20

... Oxidative stress after status epilepticus in rats inefficiency of the electron-carrying components of the mitochondrial transport chain, monoamine degradation, xanthine oxidase reaction, ... determined by analysis of variance markedly increased in this model compared with corresponding values for the control group After pilocarpine-induced SE, there was a significant (77%) increase in ... activity only changes during the initiation of seizures [14] When studying this epilepsy model, we found increased catalase activity in the hippocampus, indicating that this enzyme, in association with...

Ngày tải lên: 19/02/2014, 16:20

... increment in the protein level of GM-CSF-induced MLN51 (Fig 2E) These data indicate that GM-CSF not only induces the expression of MLN51, but also blocks the proteasome-mediated degradation of ... blocking of the proteasome degradation pathway GM-CSF-induced MLN51 is involved in the hyperproliferation and anti-apoptosis of MH7A cells via the upregulation of FLIP Next, we investigated the ... of RA FLSs, thus indicating that other factors must be involved in this proliferation In our previous studies, we have determined that GM-CSF in the synovial fluid plays an important role in the...

Ngày tải lên: 23/03/2014, 07:20

... VEGF activates the VEGF-Flt-1-FAK pathway The activation of this signaling pathway might be involved in the migration of these cells into the lesion at the site of bone destruction in GCTs We recently ... expression and the clinical stage of GCTs at presentation To determine the status of pY-Flt-1 and pY-FAK in GCT samples, the staining intensity of each specimen was scored as follows: (weak staining; less ... Immunolocalization of VEGF, Flt-1 and Flk-1 in GCT samples We initially analyzed the expression profiles of VEGF and the VEGF receptors in GCT specimens TRAP Page of staining demonstrated the presence of bone-resorbing...

Ngày tải lên: 20/06/2014, 04:20

... VEGF activates the VEGF-Flt-1-FAK pathway The activation of this signaling pathway might be involved in the migration of these cells into the lesion at the site of bone destruction in GCTs We recently ... expression and the clinical stage of GCTs at presentation To determine the status of pY-Flt-1 and pY-FAK in GCT samples, the staining intensity of each specimen was scored as follows: (weak staining; less ... Immunolocalization of VEGF, Flt-1 and Flk-1 in GCT samples We initially analyzed the expression profiles of VEGF and the VEGF receptors in GCT specimens TRAP Page of staining demonstrated the presence of bone-resorbing...

Ngày tải lên: 20/06/2014, 07:20

... the pathogenesis of EoE by enhancing activation of eosinophils through synergy with eotaxin via the ERK signaling pathway The data may be used in the possible diagnosis and prognosis of EoE In ... together with eotaxin, FGF enhances activation of eosinophils in EoE specifically Page of The increased global expression of bFGF led to the investigation of the local expression bFGF of the esophageal ... this increase is also reflected systemically In addition, the extent of increase in FGF and ERK pathways was closely associated with the severity of the disease and endoscopic findings in the...

Ngày tải lên: 08/08/2014, 21:20

... binds directly to the ODDD of HIF-1α in the cytoplasm and acetylates a single lysine residue at position 532 Acetylation of this specific lysine residue favours the interaction of HIF-1α and the ... part of the E3 ubiquitin ligase complex Interaction of prolinehydroxylated HIF-1α with pVHL/E3 ubiquitin ligase complex activates the ubiquitination machinery, thereby promoting degradation of ... HIF-1α is not ubiquitinated Thus, degradation of HIF-1α in the proteasome is inhibited and HIF-1α protein accumulates Binding of HIF-1 to HIF binding sites, formation of the transcriptional complex...

Ngày tải lên: 09/08/2014, 01:22

... protein; matrilin-1) Matrilin-3 CILP (cartilage intermediate layer protein) Fibronectin PRELP (proline/arginine-rich and leucine-rich repeat protein) Chondroadherin Fibrillin Tenascin-C Elastin MGP (matrix ... [23], linkage analysis of 14 candidate genes in OA kindreds resulted in the exclusion of 10 important cartilage genes, including COL2A1 [40] Analysis of 47 families with the phenotype of earlyonset ... epiphyseal dysplasia of several joints during childhood, and OA of the knees in adulthood A complex splicing defect of exon and/or exon 10 in the COL9A1 gene has been identified in other families [59]...

Ngày tải lên: 09/08/2014, 06:22

... arthritis dylarginine deiminase of involvement of hyaluronic of rheumatoid Proposed mechanism (PADI4) in the pathogenesis acid (HA) and peptidylarginine deiminase (PADI4) in the pathogenesis of rheumatoid ... antithrombin However, such binding did not stimulate the activity of antithrombin as did heparin and heparan; in fact, it blocked the ability of antithrombin to inhibit thrombin In the present study, the ... proteinase binding loop on the protein surface [19,20] Because the molecular structure of HA is analogous to that of heparin, HA might exert its effect by binding to the heparin-binding region of...

Ngày tải lên: 09/08/2014, 06:22

... passages in vitro before being exposed to the immunoglobulins Consequently, the data suggest that the local stromal environment in the joint (and based on previous data from the group other organs ... determines the disease specific immune response Given the variety of signaling pathways initiated by IGF-1 in fibroblasts, it may be speculated, as the authors mention briefly, that binding of antibodies ... trigger the release of IL-16 in RASFs, the present research from Smith and Cruikshank extends the panel of signals involved in the regulation of IL-16 at least under pathologic conditions Although the...

Ngày tải lên: 09/08/2014, 06:22

... result in an increase in the gene expression of the aggrecanase ADAMTS4 in cells derived from degenerate NP In vivo, the NP contains the highest concentration of aggrecan in the IVD The response of ... observed on the other cell types (Fig 6d) Discussion In this study, we investigated whether in IVD degeneration there is local production of the cytokine IL-1 and whether IL-1 could induce the cellular ... immunopositivity was observed in the matrix of the IVDs or in blood vessels, with the exception of immunopositivity for ICE, which showed some staining in the matrix and blood vessels of the most degenerate...

Ngày tải lên: 09/08/2014, 06:22

... suggesting a more general mechanism of these receptors in inflammatory diseases Maintaining autoantibody titers through FcRn-mediated recycling One of the most striking characteristics of the K/B ... through the regulation of FcRs much like the instructive role of the innate immune system for the adaptive response [56] The production of autoantibodies as a result of the failure of the immune ... anaphylatoxin, acting through the C5aR, is a major regulator of the transcription of activating and inhibitory FcγRs in IC-induced lung disease C5a increased the expression of activating FcγRIII and...

Ngày tải lên: 09/08/2014, 06:23

... extravasate into the extracellular space Once in the vicinity of the synovium, the DPPIV activity would be able to initiate amino-terminal degradation of the increased levels of proinflammatory ... levels in SF may represent an increase in the presence of attractin Certainly, analysis of serum reveals an increase in attractin in RA patients with erosive disease, which is not observed in individuals ... presence of systemic activated T cells will lead to their continuous influx into the inflamed synovium, maintaining the inflammatory state This will lead to a continuous activation of synovial...

Ngày tải lên: 09/08/2014, 07:20

... adjacent to the developing joint space The arrowhead indicates the area from which the high-magnification micrograph was taken (inset) The arrowhead in the inset indicates COMP staining (f) At ... in the pericellular space (Figure 4a) In OA cartilage taken from the area adjacent to the main defect from patients in the late stages of OA, an increase in staining intensity was found in the ... especially in the pericellular matrix of cell clusters (Figure 3b, inset) With the help of light microscopic in situ hybridization, the mRNA for COMP was detected in the cytoplasm of chondrocytes of the...

Ngày tải lên: 09/08/2014, 07:20

Ngày tải lên: 09/08/2014, 07:20

Ngày tải lên: 09/08/2014, 08:23

Ngày tải lên: 09/08/2014, 08:23

Ngày tải lên: 09/08/2014, 10:20

Ngày tải lên: 09/08/2014, 10:20