Báo cáo khoa học: "The alkylphospholipid, perifosine, radiosensitizes prostate cancer cells both in vitro and in vivo" docx

... perifosine and radiation (CTPR) on prostate cancer cells in vitro and on prostate cancer xenografts in vivo. Methods: Human prostate cancer cell line, CWR22RV1, was treated with perifosine, radiation, ... Access The alkylphospholipid, perifosine, radiosensitizes prostate cancer cells both in vitro and in vivo Yuanhong Gao 1,2,3,4,5 , Hiromichi I...

Ngày tải lên: 09/08/2014, 09:20

... (a ⁄ b) 8 barrel domain is colored in light blue and the ACMSD- specific small insertion domain in blue. The DHAP molecule and the protein residues involved in metal coordination are shown as sticks; ... the ligand O1 and O1P atoms at 2.5 and 2.8 A ˚ , respectively. Moreover, the ligand is engaged in a number of stabilizing interac- tions with the protein milieu by contacting...

Ngày tải lên: 18/02/2014, 06:20

... promoter. Sox9 binding to both binding sites (Sox9_1 and Sox9_2) within the AP-2e promoter was observed in vivo (Fig. 5C). Finally, the direct binding of Sox9 to the two Sox9- binding sites within the ... the Sox9-binding sites of the AP-2e promoter (Sox9_1 and Sox9_2). Incubation of in vitro- synthesized Sox9 with the labeled oligonucleo- tides containing the Sox9-binding sites re...

Ngày tải lên: 18/02/2014, 08:20

... (1999) Sequence analysis of scaffolding protein CipC and ORFXp, a new cohesin-containing protein in Clostridium cellulolyticum: comparison of various cohesin domains and subcellular localization of ORFXp. ... were identified, including three xylanases in F1, and several cellulases belonging to glycoside hydrolase families 9 and 5 and, a cystein protease inhibitor were identified i...

Ngày tải lên: 18/02/2014, 08:20

... the interaction between actin and myosin in the actomyosin complex [19,20]. The acetylation of the N terminus removes a positive charge and increases the interaction between actin and myosin compared ... remod- elling and is a potent inducer of cell death [4], while cytoplasmic membrane blebbing and fragmentation and apoptotic redistribution of actin were strongly inhibited in...

Ngày tải lên: 20/02/2014, 23:20

... analyzed by western blotting using anti- bodies to RasGRF1 and a-tubulin. A B Fig. 5. DHPH2 domain of RasGRF1 binds directly to microtubules. In vitro binding of recombinant regions of RasGRF1 ... scaffold protein for the Jun N-ter- minal kinase signaling pathway. JIP proteins also link the motor proteins kinesins with the cargo complex to be transported along the microtubules [34]. In...

Ngày tải lên: 07/03/2014, 12:20

... forms of T antigen which included the J domain, the Rb-binding domain and the DNA-binding domain, but lacked the ATPase domain of the C-terminus. Given that the ATPase domain of the T antigen has ... embryos, respectively, were maintained in minimal essential medium supplemented with 10% fetal bovine serum at 37 °Cina humidified 5% CO2 atmosphere. Sf9 insect cells were grown in Sf900II...

Ngày tải lên: 16/03/2014, 05:20

... between rhodopsin and ABCA4 for 11-cis-retinal binding. In the absence of ABCA4 this competition would be removed, increasing the availability of 11-cis-retinal to rhodop- sin and increasing the speed ... retinoid in this path- way has a specific chaperone to prevent unwanted reactions [25]. For instance rhodopsin has a total of three binding sites for retinoids, allowing one 11-cis- r...

Ngày tải lên: 22/03/2014, 15:21

... distinct core pro- teins, H4 and HMGN2, resulting in the induction of LL-37, a member of the antimicrobial peptide that protects the urinary tract against invasive bacterial infection [43]. In ... Gram-negative bacteria, fungi and viruses such as in uenza viruses. b-defensins are largely expressed in epithelial cells on the surface of respiratory, gastrointestinal and geni- tou...

Ngày tải lên: 22/03/2014, 16:20

... studied represen- tative actinoporins are equinatoxin II (EqtII), isolated from the sea anemone Actinia equina, and sticho- lysin II, from Stichodactyla helianthus. Actinoporins form cation-selective ... molecule and the C-terminal a-helix [20,22,23], and by a recently defined phosphorylcholine-binding site [19]. In the next step, the N-terminal a-helix detaches from the core of the mo...

Ngày tải lên: 23/03/2014, 10:20