Báo cáo khoa học: " Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response" potx

... of 13 RESEARCH Open Access Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response Pei-Ming ... design DNA- directed alkylating agents by linking the alkylating pharmacophore to the DNA- affinity molecules (e.g., DNA intercalating agent...

Ngày tải lên: 09/08/2014, 09:20

... transduc- tion in both cell lines (data not shown). In order to test the enzymatic activity of the Dm-dNK–GFP fusion protein and the level of nucleo- side kinase activity in the cells, we determined the ... retained enzymatic activity in these human T-cell lines. Increasing sensitivity to nucleoside analogs in Dm-dNK expressing cells We determined the sensitiv...

Ngày tải lên: 20/02/2014, 01:20

... erythrocytes, and the difference between acetylcholinesterases of kid- ney and erythrocytes in the extent of binding with the lectins concanavalin A, Lens culinaris agglutinin (LCA), and Ricinus communis ... mRNA, and the b-actin mRNA, used as the internal standard. The sequence and position of the prim- ers, as well as the size of the PCR products, are p...

Ngày tải lên: 06/03/2014, 22:21

... kit (Invitrogen, USA). The quantity and quality of RNA was determined by the OD measurement at 260 and 280 nm. The integrity of RNA was checked by visual inspec- tion of the two rRNAs 28S and ... normal condition, the majority of glutathione is in the reduced form. Shifting redox equilibrium is in favor of a redu- cing or oxidizing state; that is in modif...

Ngày tải lên: 10/08/2014, 10:21

... increase of GTPcS binding was completely inhibited by Fig. 2. Binding of muscarinic ligands. All experiments were performed in triplicate. Each data point represents the mean ± SEM. (A) Binding ... over the detection sensitivity in the western blot analysis and well transferred in the membrane fraction. Binding properties of muscarinic ligands with M 2 ::GOA-1 fusion p...

Ngày tải lên: 07/03/2014, 11:20

... analyzed, the changes observed allow the generation of hypotheses and the design of further experiments validating the initial findings presented here and investigating the functional relevance of microRNA ... predict the efficacy of therapeutic interventions, including radiother- apy [8,9]. In fact, microRNAs have been shown to mo d- ulate the radiosensitivity...

Ngày tải lên: 09/08/2014, 09:20

... prominently observed by Hoechst 33342 staining in these cells (Fig. 3A), and the rate of apoptotic cells was approximately threefold higher in the Hsp105a over-expressing cells than in F9 or V1 cells ... determine the effects of Hsp105a on JNK and p38 signalling pathways, we examined the activation of JNK and p38 in F9 cells by oxidative stress. As sh...

Ngày tải lên: 21/02/2014, 03:20

... assessed by routine staining with hematoxylin and eosin, and examined by light microscopy. The inflammatory response was evaluated by examining the lung tissue for the presence of typical in- flammatory ... immune system by interacting with intestinal epithelial cells in the presence of butyric acid, a metabolite produced by intestinal bac- teria [29]. In...

Ngày tải lên: 07/08/2014, 20:23

... phases of the interpretation of the sentence "Bob met Sue and Mary in London". H means "head" and indicates the position of the node filler within the sequence of dependent ... new pieces of information, which were implicit in the "linear" input form, are made explicit in the result of the analysis. These pieces of in...

Ngày tải lên: 21/02/2014, 20:20

... goal of our research is to identify the role of verbs, keeping in mind that event profile is but one of many factors in determining text type. In our study, we explored the contribu- tion of ... they were commonly post- ings of company earnings reaching a new high or dropping from last quarter. Agreement and argument verbs appeared in many of the same artic...

Ngày tải lên: 08/03/2014, 05:21