... important step in developing analyses usingRLGS profiles is the cloning of target spots. However,the amount of DNA available for cloning in a singlespot is very small. For example, 1.5 lg of mouse ... fragment containing the origin or end point of each sequence might not be valid because eachsequence (clone) may be divided from a single longsequence. Therefore, we added information to indicateif ... methylation. In plant DNA, 5¢-methylcytosine (5mC) occurs atcytosine residues in symmetrical sequences, CpG andCpNpG (where N is any nucleotide), due to theactions of MET1, DRM1 ⁄ 2 and CMT3 [20–22]. In Arabidopsis,...