... Forward primerAcs 5¢-TAATACGACTCACTATAGGGA*5¢-AACACACCATTCCTGCCAAC-3¢CCACCACAGGTCGCGCC-3¢AcnB 5¢-TAATACGACTCACTATAGGGA*5¢-CTCACACGCTGCTGATGTTC-3¢CGTGGTTACGCACTTCACC-3¢PrpD 5¢-TAATACGACTCACTATAGGGA*5¢-AACATCGGCGCGATGATCC-3¢TCGCTGCTTCAACTGCCG-3PrpE ... pyroglutamicacid formation at the N-terminal glutamine, and modifica-tion of cysteine by carbamidomethylation as well as partialcleavage leaving a maximum of one internal site uncleaved.RNA isolation and Northern ... trans-aconitate,D-malate andL-malate, fumarate, maleate,D-tartrate and meso-tartrate,D-citramalate andL-citramalate,mesaconate, citraconate, itaconate, and (R,S)-3-methylitaconate.SubstrateConcentration(mM)ActivityUÆmg)1%(2S,3S)-Methylcitrate...