Báo cáo y học: "The p38 mitogen-activated protein kinase signaling cascade in CD4 T cells" docx

... regulate mRNA stability is through the phosphorylation and inactivation of the zinc finger protein TTP. Binding of TTP to AREs in the 3′ untranslated region leads to the rapid degradation of target ... helper; TNF = tumor necrosis factor; TTP = tristetraprolin. Available online http://arthritis-research.com/content/8/2/205 Abstract Since the identification of the p38 mitogen-activated...

Ngày tải lên: 09/08/2014, 07:20

... upregulated by p38 inhibitor, but not altered by the ERK1/ERK2 pathway. To study whether the MAPK pathway regulates progestin-induced gene transcription, MCF-7 cells were treated with MPA with and ... pathway mediates the progestin effect on the cell cycle regulatory molecules during growth inhibition. Thus our results establish a candidate gene that may be involved in progestin-induced g...

Ngày tải lên: 24/03/2014, 00:21

... CtBP can also bind sites within the N-terminal domain of Eos. TRPS1 interacts with CtBP The TRPS1 protein is a multi-(zinc finger) protein that contains two C-terminal fingers highly related to ... interaction. In this way, it seems likely that proteins such as Eos may be able to bind to CtBP that is already associated with another PXDLS motif-containing protein. For instance, Eos may bi...

Ngày tải lên: 21/02/2014, 01:21

... Rac in eluted fractions at each of the three steps of the protein fractionation was determined by immunoblotting. Immunoprecipitation assay The cytosolic extract from nonactivated bovine neutrophils was ... immunoprecipitation and protein fractionation whether the cytosolic phox proteins could bind to S100A8/A9. Fol- lowing incubation of a cytosolic extract from nonactivated bovine neut...

Ngày tải lên: 18/03/2014, 01:20

... electro- static interactions with the lipid layers. Our results indicate that the penetration of the protein into the lipid monolayer is also in uenced by the hydrophobic interactions between APP ... solubility of the protein in the bulk phase at high ionic strength, leading to a greater amount of the protein present at the interface. In other words, increasing the ionic strength h...

Ngày tải lên: 31/03/2014, 21:21

... shift titration experi- ments with purified proteins, presented in this report, demonstrate unambiguously that no direct protein- pro- tein interactions between HIV-1 RT and HuR are present in vitro ... a direct protein- protein interaction between HuR and HIV-1 RT in this study, an indirect interaction may be mediated by RNA. However, we could not detect any influence of HuR on HIV-1...

Ngày tải lên: 12/08/2014, 23:23

... full-length HIV-1 Tat obtained by in vitro translation. All deletants lacking the N-terminus of the protein up to aa 161 bound Tat as efficiently as the full length protein; in contrast, binding was ... protein- protein interactions. Over the last few years, a number of cellular proteins have been reported to interact with Tat and to mediate or modulate its activity. Among these inter...

Ngày tải lên: 13/08/2014, 06:20

... team at the moment of consultation. This methodology captures important opportunities for improvements in local care at a rate far higher than routine incident-reporting systems, but without requiring substantial ... requiring substantial investments of additional resources. Academic centers are increasingly recognizing engagement in quality improvement as a distinct career pathway. Invol...

Ngày tải lên: 25/10/2012, 10:06

... IRSs through their src homology 2 domains (a) the growth factor receptor binding protein activates the ras/ mitogen-activated protein kinase pathway and (b) phosphatidylinositol 3 -kinase activates ... phosphatidylinositol 3 -kinase inhibitor, wortmannin, inhibits insulin-induced activation of phosphati- dylcholine hydrolysis and associated protein kinase C transloca- tion in...

Ngày tải lên: 20/02/2014, 02:21

... ferritin (data not shown). Experiments incubating the lysate with an anti-ferritin antibody demonstrated that only this band could be super- shifted (Fig. 1B), again indicating that it was ferritin. ... m M ). This latter agent is a potent and selective inhibitor of the enzyme c-glutamylcysteine synthetase that is involved in GSH synthesis [40]. A 20-h incubation with BSO at a concentration...

Ngày tải lên: 08/03/2014, 22:20