Báo cáo y học: "Proliferation and differentiation potential of chondrocytes from osteoarthritic patients" ppt
Báo cáo y học: "Proliferation and differentiation potential of chondrocytes from osteoarthritic patients" ppt
... at days 7 and 14. The othersamples were only weakly positive at day 14.To test the potential of using Hyaff-11 as a scaffold for thedelivery of chondrocytes, the scaffold was seeded from thetop ... ability of the culture-expanded cells to form collagens inthe pellet model was analysed biochemically and by immuno-reactivity to type I and type II collagen (Fig. 3). The totalamounts of collagen ... seeded with ACT chondrocytes. Attached to the hyaluronic acid, the chondrocytes redifferenti-ated within the scaffold, as seen by the secretion of proteogly-cans and the synthesis of type II collagen...
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Báo cáo y học: "Behavioral and antioxidant activity of a tosylbenz[g]indolamine derivative. A proposed better profile for a potential antipsychotic agent" pptx
... activity.Materials and MethodsSynthesis of TPBIAKey step of the synthesis was a Mukaiyama type aldol con-densation between the dimethyl acetal of 1-(p-toluenesul-fonyl)pyrrole-3-acetaldehyde and 4-di-n-propylamino-1-trimethylsilyloxycyclohexene ... purposes)Annals of General Hospital PsychiatryOpen AccessPrimary researchBehavioral and antioxidant activity of a tosylbenz[g]indolamine derivative. A proposed better profile for a potential antipsychotic ... of 40 and 80µmol/kg reduces the activity by 39 and 82% respectively(Number of experimental animals: 3–6).ii. In apomorphine pretreated rats, TPBIA (80 µmol/kg)reverses the hyperactivity and...
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Báo cáo y học: " Derivation and preliminary validation of an administrative claims-based algorithm for the effectiveness of medications for rheumatoid arthritis"
... by the algorithm from 30 and 60%, the amount of bias compared to the true response rate ranged from 1 – 21%. The results of the second sensitivity analysis that had no baseline VARA visit (and ... Sensitivity and positive predictive value of Medicare Part B physician claims for rheumatologic diagnoses and procedures. Arthritis Rheum 1997, 40:1594-1600. 19. Singh JA, Ayub S: Accuracy of VA ... authors made substantial contributions to conception and design, and to the analysis and interpretation of the data. TRM and GWC handled acquisition of data. All authors contributed to the manuscript...
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Báo cáo y học: " Induction and effector phase of allergic lung inflammation is independent of CCL21/CCL19 and LT-beta
... airway hyperreactivity but cause severe airway inflammation. J Clin Invest 1999; 103: 175-83. [32] Cohn L, Tepper JS, Bottomly K. IL-4-independent induction of airway hyperresponsiveness by Th2, ... intranasal challenge. Briefly, mice were maintained inside a whole-body plethysmograph (Buxco, Troy, NY) capable of meas-uring changes in airflow and trans-thoracic pressures and resistances [30]. ... presentation of antigen in the lung parenchyma might be sufficient to trigger the cascade of cytokines and chemokines necessary for tissue eosinophilia without involvement of draining lymphoid...
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Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
... insects.Parabutoporin and opistoporin 1 were synthesizedchemically and preliminary studies on antibacterial activityshowed that the quality and biological activity of native and synthesized toxins ... in17.5 min.Isolation of human granulocytesHuman granulocytes were obtained from the blood of healthy volunteers and purified by Ficoll–Paque centrifu-gation and hyposmotic lysis of red blood cells ... activity assay. Antifungal activity of the pep-tides was assayed by microspectrophotometry of liquidcultures grown in microtiter plates as described previously[33]. Brie y, in one well of a...
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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf
... Molecular Biology, University of Debrecen, Hungary;3Department of Inorganic Chemistry, and 4Research Group for Antibiotics, Department of Chemistry, University of Debrecen, Hungary;5International ... Ko¨ve´r3, Gyula Batta4 and Jacek Kuz´nicki1,51Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland;2Department of Biochemistry and Molecular ... oligomeric states of CR I–II and Calb I–II are an indication of differentorganizations of EF-hands within full-length CR and Calb.CR I–II and Calb I–II may have different structuresCR I–II and Calb...
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Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx
... Sequence analysis and molecular modellingpredict the two EF-hand motifs characteristic of theS100 family. Metal-binding properties were studied by adirect45Ca2+-binding assay and by fluorescence ... [10]. Hofmann et al. [11] proved thatS100 A12, and possibly other members of the S100 family,mediates the activation of a novel proinflammatory axis bybinding to RAGE (receptor for advanced glycation ... to sequencing by MS/MS. A summary of thesequence analyses and the resulting primary structure of lungfish calhepatin is shown in Fig. 3.The protein is composed of 75 residues. From its amino-acid...
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Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx
... ability of Tomo H to transform p-cresol into 4-MC, inthe absence of any capability of Tomo D to transferelectrons, can be attributed to its capacity to modulate theactivity of the hydroxylase ... ratios of Tomo C and D followed by a sharp break atabout 1.6 mol of Tomo C per mol of Tomo H and 3 mol of Tomo D per mol of Tomo H (Fig. 5A,B), respectively. Thenearly linear titration and the ... enzymatic systems able to activatearomatic rings by mono- and di-hydroxylations and tooperate ortho or meta-cleavage pathway [1,2] which leads tocitric acid cycle intermediates.Toluene/o-xylene-monooxygenase...
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Báo cáo Y học: Structural and serological relatedness of the O-antigens of Proteus penneri 1 and 4 from a novel Proteus serogroup O72 pptx
... Arbatsky2,Alexander S. Shashkov2 and Yuriy A. Knirel21Department of General Microbiology, Institute of Microbiology and Immunology, University of èodzÂ, Poland;2N. D. ZelinskyInstitute of Organic ... Microbiology, University of èoÂdz (Poland). Thirty-seven strains of P. mirabilis and 28 strains of P. vulgaris were from the Czech NationalCollection of Type Cultures (CNCTC, National Institute of ... containing one residue each of D-Glc and D-Gal and twoD-residues of GalNAc.The1H- and 13C NMR spectra of the polysaccharidewere assigned using 2D COSY, H,H-relayed COSY, and H-detected1H,13CHMQCexperiments(Tables 1and2 ).Signals...
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Báo cáo Y học: Synthesis and turn-over of the replicative Cdc6 protein during the HeLa cell cycle potx
... begins, yeastCdc6p is phosphorylated and then rapidly destroyed b y ubiquitin-mediated protein degradation. The regulateddestruction of Cdc6p effectively prevents the binding of Mcm proteins, and ... (0–2 h afterthymidine-block) and in the middle of S phase (4–6 h), aswell as at the e nd of mitosis and during the early G1 phase(12–14 h) of the next cycle (Fig. 2A).Cytoplasmic and chromatin ... fluctuate across the yeastcell cycle. They rapidly decrease w ith the entry of yeast cellsinto S phase and increase again during t he following G1phase with the synthesis of new Cdc6p.In contrast,...
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