... residues B2and E9 of 7.35 A˚in de Sanctis’ structure (pdb 1UM0Reduced CygbOxidized CygbReduced CygbABCFig. 2. ESI-MS analyzed under denaturing conditions of: (A) a solution of reduced ... oxidized(intramolecular disul de bond) form and a reduced(disul de free) Cygb form is evident in the kinetics;such a transition affects the E-helix position, allowinga fine modulation of the endogenous ... Healthcare, Life-sciences, Uppsala, Sweden) on a Hitrap DEAE Sepharosecolumn (GE Healthcare, Lifesciences, Uppsala, Sweden).The concentrated material was loaded onto a Superose 12HR 16 ⁄ 50...