Báo cáo khoa học: "Micronutrients in biomass fractions of holm oak, beech and fir forests of the Montseny massif (Catalonia, NE Spain)" pot

... functional proteins is determined in a phospholip id probing assay. The detection of protein–protein and protein–lipid binding [38] involves a two-step process. First, the sample used to probe the array ... the addition of C- or N-terminal tags, such as glutathione-S-transferase or the IgG-binding domain of Protein A. The incorpor- ation of fusion tags also facilitates...

Ngày tải lên: 23/03/2014, 15:21

Ngày tải lên: 07/08/2014, 18:21

... stabili- ties of interaction with the pre-60S complex and not inefficient binding to the column. In order to determine whether the Nop53p trun- cated mutants also bind RNA directly, in vitro RNA binding ... Maintenance and handling of E. coli and yeast strain Escherichia coli strains DH5a and BL21(DE3) were main- tained in LB medium and manipulated according to standa...

Ngày tải lên: 30/03/2014, 04:20

... from the acyl chains of PtdCho or sphingomyelin; however, neither the choline headgroups of PtdCho and sphingomyelin (except for a small peak in THP-1) nor the sphingosine chain of sphingomyelin ... fraction. The rest of the signal was found in the membranes and low speed pellet (which includes unbroken cells and organelles). The contribution of the insolub...

Ngày tải lên: 23/03/2014, 17:21

... most frequently in the arid western and northwestern region, and vertisols dominate the lowlands of the plains of the northern Gulf of Mexico. The Tamaulipan thornscrub forests and its different ... characterized as litosols and rendzins dominate the hilly slopes of the eastern Sierra Madre mountain range and the smaller mesetas on the plains of the...

Ngày tải lên: 08/08/2014, 14:20

... under- stood, the data obtained in the present study indicate that the 43 residues (amino acids 702–744 in mice) that reside in the middle region of the CD act as the v-KIND binding core. The v-KIND-binding ... specific MAP2 binding resides within the 32 residues (amino acids 456–487) of the v-KIND KIND2 domain. To examine whether the MAP2 binding core site of v...

Ngày tải lên: 14/02/2014, 19:20

... to that in Asp141 of Met8P. The idea of NirF being a dehydrogenase is appealing because of the presence of a putative nucleotide-bind- ing motif in the N-terminal of the protein sequence and also ... and of the kanamycin insertion mutant nirF strain (SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom left panel and of SB...

Ngày tải lên: 15/02/2014, 01:20

... separately for the numerator and the denominator of Eqn (5). Based on the SD of the numerator and the denominator the SEM of q h was computed, assuming statistical independence of the two. The time-dependent ... pre- ceded by a significant increase in transcript level. In the case of PYK, one isoform increased and the other decreased at the mRNA level....

Ngày tải lên: 18/02/2014, 06:20

... is the major interaction site for the adenine moiety. In b1, an arginine residue (Ile417 in rPDE2) binds to N1 and a threonine (Ser419 in rPDE2) inter- acts with N7 and the N 6 amino group. The ... adenine and guanine of cAMP and cGMP as primary ligands. Previous experiments investigating cGMP binding to GAF domains of mammalian PDEs were designed on the basis of...

Ngày tải lên: 19/02/2014, 02:20

... 4705 In the p21 50-mer target and its derivatives p21a and p21b, the 5¢-neighboring guanines in the ‘top’ strand form another GG doublet with the first guanine of the p53DBS (Fig. 1). Interestingly, ... accommodate the cisplatin IACs. The higher the probability of formation of the cisplatin IACs within the p53DBSs due to the occurrence of the GG, AG and...

Ngày tải lên: 19/02/2014, 05:20