Báo cáo khoa học: "Molecular structure and biochemical properties of lignins in relation to possible self-organization of lignin networks" pdf

... components. In an attempt to relate the formation of such lignin net-works to possible functions of lignins, anew hypothesis on the self-organization properties of lignin is ... datarelated to the variations in the molecular structure of lignin and mainly discussesthe biochemical heterogeneity and occur-rence of associations between lignins and other ... determined by mercurization withSEM- and TEM-EDXA. Wood Sci. Technol. 22,251-257Molecular structure and biochemical properties of lignins in relation to possible self-organization...

Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

... ‘stored’ in the autoinjector).Analyses of the degradation of a-chitin and b-chitin wereconducted by incubating 100 lL solutions containing1mgÆmL)1 of b-chitin (squid pen b-chitin, 3 lm in ... N-terminal chitin-binding domain. ChiG, onthe other hand, lacks this chitin-binding domain, and consists only of a catalytic domain [22]. The chiG geneencodes a 244 amino acid chitinase, including ... corresponds to residue 8 in ChiG [18]. In ChiC, this N-terminus ispart of a linker (with unknown structure) that connectsan N-terminal chitin-binding domain to the catalyticdomain. All in all, it is...

Báo cáo khoa học: Quaternary structure and functional properties of Penaeus monodon hemocyanin docx

... throughout.Analysis of oxygen-binding curvesMWC modelThe oxygen-binding curves of hexameric Hc from P. mono-don were analysed according to the MWC model using thefollowing binding polynomial:Pmwc¼ðl ... out in 50 mM Tris ⁄ HCl, 20 mM CaCl2,pH7.5(solid line). The dashed line and the dotted line indicate the elutionproﬁle of a further chromatography of the material included in peakA and ... conformational state is needed to describethe oxygen-binding behaviour of Penaeus monodon hemocyanin within thepH range of 7.0 to 8.5. The dodecamers bind oxygen according to the nes-ted Monod–Whyman–Changeaux...

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

... b-conglycinin and glycinin,as pro-b-conglycinin and proglycinin are transient pro-tein forms that are present in the ER prior to process-ing in the protein storage vacuoles. The synthesis of proglycinin ... redox-activeCGHC motifs within the a-domain and a¢-domain.Instead, they both contain the sequence CPRS in thea-domain and CMNC or CINC in the a¢-domain.GmPDIL-3a and GmPDIL-3b contain a C-terminalKDEL sequence ... proteins play important roles in folding and quality control of nascent polypeptides[25]. In soybean cotyledon, large amounts of seed stor-age proteins such as glycinin and b-conglycinin aresynthesized...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu-bating the protein at 60, 70 and 80 °C for 30 min and ... a5 and a6 helices in thepRN1 prim–pol protein. Because of a two-residue gap in the corresponding region of the Rep245 sequence,this ﬁnding had not been predicted in phd and profsecondary structure ... that is plausibly involved in DNArecognition and binding and is positioned left of the active site [14].Rep245 is both capable of de novo synthesis of DNA primers and of elongating them. Long...

Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

... with cerebral malaria and in the brain of a murine model of the syndrome[18,19]. Interestingly, picolinate is reportedly able to prevent the neurotoxic effects of quinolinate in the ratcentral ... restored the bacterial protein [21,33].Interestingly, the same difﬁculty in stripping off themetallocofactor from the protein has been describedfor murine adenosine deaminase, a member of ... duplicated in experimental animals byintrastriatal quinolinate injection [5,6]. In addition, asigniﬁcant elevation of quinolinate levels has beenobserved in low-grade Huntington’s disease brains,suggesting...

Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt

... interaction.Thus, in terms of inhibition of cell adhesion, cVY has morepotential since it may directly interrupt the binding mech-anism of CD2–CD58. Inhibition of cell adhesion involvesinterrupting the interaction ... (Cvff). To eliminate the steric hindran ce betweenpeptide and protein, and to relax the hydrogen added to theprotein, the peptide and protein were merged and minimizedbefore docking. A tomic ... (w/v) heat-inactivated fetal bovine serum and 100 mgÆL)1 of penicillin/streptomycin. Caco-2 cells were maintained in minimumessential m edium-a containing 10% (w/v) f etal bovineserum, 1%...

Tài liệu Báo cáo khoa học: The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB pdf

... identiﬁcation of lipopolysaccharides and immunodeterminan ts in pathogenic strains of Haemophilis in u-enzae; application to clinical isolates. In Mass Spectrometry in Biology and Medicine (Burlingame, ... cm) and Sephadex G -15columns (1.6 · 80 cm) in pyridinium-acetate buffer,pH 4.5 (4 mL of pyridine and 10 mL of AcOH in 1 L of water), and the eluate was monitored by a refractive indexdetector.Conﬁguration ... collected into tubes containing heparin and d iluted 1 : 4 in RPMI 1640. Sa mples were a liquotedinto 96-well plates, agonist was added, and incubatio n wascarried out at 37 °C in an atmosphere of...

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... participating in an IgE-bindingepitope. The N-terminal sequencing of a 52-kDa glyco-protein of tomato extract gave hints for the existence of b-fructofuranosidase as a relevant allergen in tomato[15,16]. ... recombinant pro-teins (both isoforms) were separated by SDS/PAGE (0.5 lgFig. 1. IgE binding to glycoproteins in tomato extract. IgE-binding of sera from German (A) and Spanish (B) patients to ... 20 and 30 kDa showing enhancedreactivity in skin prick tests (SPT). Darnowski et al.[6]investigated the distribution of proﬁlin in tomato tissues.Recently the cDNA sequence of tomato proﬁlin...

Tài liệu Báo cáo khoa học: "The Structure and Process of Talking About Doing" pdf

... model of the role of point of view in problem solving. SUMMARY We have reported here a three pronged approach to the study of problem solving action and report: I) the collected of data ... "point of view" for a problem solver at each point in the problem solving from a record of the problem solving report and a record of moves made. Then, we use this extracted trace to ... collected of data on problem solving and talk about problem solving, 2) development of a process model of these behaviors, and 3) use of coding techniques to extract traces of "critical phenomena"...

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