... of endogenous plant growth substances in Douglas fir I. Cytokinin analysis N. Imbault P. Doumas C. Joseph M. Bonnet-Masimbert 2 1 Laboratoire des Composes Ph6noliques, Université ... this paper, some of their applications to the woody plant, Douglas fir (Pseudotsuga menziesii Mirb.), are presented: purifica- tion by immunoaffinity chromatography (...
Ngày tải lên: 09/08/2014, 02:21
... and sensitive detection, identification and quantification of some GAs. C18 purifica- tion and directly labeled antibodies de- crease the number of steps required and improve ... GAs of Douglas fir shoots provide a clear illustration of the utility of a com- bined HPLC-ELISA detection system for GAs. This method allows rapid, specific Pharis...
Ngày tải lên: 09/08/2014, 02:21
Báo cáo khoa học: Study of synthetic peptides derived from the PKI55 ppt
... Dipartimento di Medicina Sperimentale e Diagnostica, Sezione Genetica Medica, Universita ` degli Studi di Ferrara, Italy 2 Dipartimento di Biochimica e Biologia Molecolare, Universita ` degli Studi di Ferrara, ... Ferrara, Italy 3 Dipartimento di Studi Farmaceutici, Universita ` di Roma ‘La Sapienza’, Italy 4 Dipartimento di Scienze Farmaceutiche, Universita ` degli Studi di Ferrara, Italy 5...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... PSI can be made in planta. In future experiments, we will evalu- ate its use for quick purification of PSI and alignment of PSI particles during structural determination of PSI using electron microscopy ... test the feasibility of rescuing Arabidopsis PSI com- plexes lacking PSI-G with tagged constructs. Successful introduction of a hexa-histidine (His)- or Strep-tagged PSI-G into PS...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
... min and then incubated on ice for 5 min. Plasmid–peptide dissociation and plasmid purifica- tion was carried out with a commercially available DNA purification kit (QIAquick PCR purification kit; ... Takeuchi T, Katoh H, Hiramoto K, Negishi M, Nomizu M, Suguira Y & Futaki S (2007) Interaction of arginine-rich pep- tides with membrane-associated proteoglycans is crucial for induction of a...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Activity of the plant peptide aglycin in mammalian systems potx
... a cross-kingdom bioactive peptide family. Interestingly, similarity searches using the Swiss Institute of Bio- informatics (SIB) BLAST network service (http:// www.expasy.org/cgi-bin/blast.pl) identified ... concentration. VDAC-1 was purified and identified as a specific aglycin binding protein. A high-affinity protein binding site for aglycin in the gut of cereal weevils has been Aglycin acti...
Ngày tải lên: 23/03/2014, 09:21
báo cáo khoa học: "Induction of endogenous γ-globin gene expression with decoy oligonucleotide targeting Oct-1 transcription factor consensus sequence" pps
... of the competitive binding of the Oct-1 decoy oligonucleotide in vitroFigure 1 Determination of the competitive binding of the Oct- 1 decoy oligonucleotide in vitro. The radioactively labeled ... target genes, it will significantly extend its poten- tial applications. Induced transcription of target genes with decoy oligonucleotides will also have important clin- ical implications...
Ngày tải lên: 10/08/2014, 22:20
Báo cáo khoa hoc:" Study of the impact of perilipin polymorphisms in a French population" docx
... was associated with increased obesity risk in Malays and Indi- ans, but not in Chinese [16]. In this study, we examined the genetic variability of the perilipin gene and possible associations with ... AS, et al.: Intra- genic linkage disequilibrium structure of the human perilipin gene (PLIN) and haplotype association with increased obes- ity risk in a multiethnic Asian population. J Mol...
Ngày tải lên: 11/08/2014, 08:20
báo cáo khoa học: " Construction of a potato consensus map and QTL meta-analysis offer new insights into the genetic architecture of late blight resistance and plant maturity traits" pps
... meta-QTL Maturity meta-QTL VII I VIII II IX III X IV XI V XII VI Figure 3 Graphical overview of the late blight and maturity meta-QTLs. The 12 consensus potato chromosomes are represented by 12 vertical ... chro- mosomes III, V, VII, VIII and XI), while the confidence interval of individual QTLs varied greatly. In these regions, the consensus map appeared cond ensed in com- parison with...
Ngày tải lên: 11/08/2014, 11:21
báo cáo khoa học: " Study of ‘Redhaven’ peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism" ppsx
... Kobayashi M, Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K, Yoshida S, Asami T: A novel inhibitor of 9-cis-epoxycarotenoid dioxygenase in abscisic acid biosynthesis in higher plants. Plant Physiol ... 14 Additional material Additional File 1: Structures of the main carotenoids identified in RHB and RH fruits during ripening. Carotenoid composition is reported in Table 1. Additional Fi...
Ngày tải lên: 11/08/2014, 11:21