... sensitivity of hepsin and matriptase, and the relative insensitivity of matriptase-2 and DESC1, to leupeptin are noteworthy. Moreover,when matriptase activity was tested in the presence of proteins of ... (substrates 6, 8 and 10) was tolerated and yielded kcat⁄ Kmvalues that were similar to that of the reference (substrate 1). Leu and Tyr (substrates 12 and 14) at P2, and Ala, Gln and Tyr (substrates ... effects of various proteaseABFig. 1. TTSP expression and purification.(A) Schematic representations of matriptase,matriptase-2, hepsin and DESC1. Arrows and numbers indicate the first and lastamino...