... Singapore, Singapore2 Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore3 Department of Medicine, Yong Loo Lin School of Medicine, National ... the bestmethod for the high-resolution separation of a com-plex mixture of proteins, and its efficacy in distinguish-ing post-translationally modified proteins and proteinisoforms is unparalleled. ... limitations of 2Delectrophoresis. These include bias to abundant pro-teins, limitations in resolving certain classes of proteinsand difficulty in producing reproducible 2D gels[123,142]. Because of...