Báo cáo khoa học: "Assessment of the spatial distribution of light transmitted below young trees in an agroforestry system S Meloni" pptx
... distri- bution between 30 and 90% was not signif- Original article Assessment of the spatial distribution of light transmitted below young trees in an agroforestry system S ... the spatial distribution of transmitted radiation. Tree structure and light distribution below trees were measured in an agroforestry sys- tem consi...
Ngày tải lên: 08/08/2014, 18:21
... negatively numbered subsites of IPU also resembles that of GH family 49 dextranases. Materials and methods Host strains and media Escherichia coli JM109 was used for the plasmid con- structions. P. pastoris GS115 ... structure of Dex49A using the Swiss- Model server. IPU consists of a signal sequence (residues 1–19) and a mature part (residues 20–564), and the model is composed...
Ngày tải lên: 19/02/2014, 16:20
... measure, and the proportion of TPs among this -best list (the precision) is computed. Another measure of goodness is the proportion of TPs in the base data that are also contained in the -best ... evaluation of lexi- cal association measures, mainly for the follow- ing reasons: the instability of precision values ob- tained from the first few percent of the da...
Ngày tải lên: 20/02/2014, 18:20
Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
... A 0 is the intrinsic anisotropy. This analysis starts with an initial model of the G (s, h) distribution as a ÔflatÕ mapwhereallthe (s, h) are equiprobable. A value of the intrinsic anisotropy of ... domain of B. pertussis CyaA toxin. R224 is the site of trypsin cleavage of the protein in the two subdomains. I, II and IV correspond to those segments possessing hig...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx
... acids (Fig. 1A). A thiazoline ring is present at the N-terminus of the pep- tide formed either by l-cysteine and l-isoleucine or by l-cysteine and l-valine. The various analogs result from substitutions ... bacitracin A. The thiol form of the thiazoline ring of bacitracin A and Cys345 are shown as chemical structures, the rest of bacitracin A as ‘Bacitracin A’, and all oth...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Differential tissue-specific distribution of transcripts for the duplicated fatty acid-binding protein 10 (fabp10) genes in embryos, larvae and adult zebrafish (Danio rerio) docx
... RT-PCR, and Santhosh Karanth for providing technical assis- tance during the course of this study. This work was supported by research grants from the Natural Sciences and Engineering Research ... the basis of the distribution of fabp10b transcripts in many tissues of adult zebrafish, compared with the limited distribution of zebrafish fabp10a transcripts, and chicken...
Ngày tải lên: 16/03/2014, 00:20
Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
... Expression of anti-WASP scFvs and in vivo interaction between scFvs and WASP in scFv transgenic mice T and B cells. (A) Western blot analysis of protein extracts of T and B cells from the spleens ... scFv intrabodies and assessed their binding activity to the WASP-EVH1 domain in the scFv gene-transfected T cells. Finally, we succeeded in expressing the functional scFv in...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx
... signal strength; the dur- ation of the signal; the final signal strength and the integral signal strength. The summing is over all the processes in the system, and its results are given in the final ... the kinases or phosphatases in the system, and whether they are controlled differ- entially by some or all of them. On the basis of the antagonistic action...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx
... Diseases. We greatly appreciate the expert technical assistance of Ali Sepulveda Mun ˜ oz in expression and purification of the recombinant enzymes, and the staff of the Swiss-Norwegian Beamlines ... mechanism inclu- ding its reversal by substrate binding. Although the pterin cofactor and the substrate bind at distinctly different sites, the local conformational changes imp...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Curcumin suppresses the dynamic instability of microtubules, activates the mitotic checkpoint and induces apoptosis in MCF-7 cells ppt
... monopolar spindles (Fig. 5C). Discussion In this study, we have provided several lines of evi- dence indicating that the antiproliferative mechanism of action of curcumin involves the perturbation of microtubule ... acquisition and processing. MCF-7 cells were transfected with EGFP– a-tubulin plasmid, as described previously [20] and the stably transfected MCF-7 cells were mai...
Ngày tải lên: 23/03/2014, 03:20