Báo cáo y học: " pdf

... Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phospho enol pyruvate carboxykinase determined by fluorescence spectroscopy Marı ´ a ... solution. Keywords: Escherichia coli phosphoenolpyruvate carboxy- kinase; ligand binding; conformational changes; P-pyridoxyl fluorescence spectroscopy. Escher...

Ngày tải lên: 21/02/2014, 01:21

... cooperation of Dnmt3a and Dnmt1 in de novo methylation of DNA that can only be provided by in vitro experiments. In this model Dnmt3a is targeted to a domain of the DNA that is subject to de novo methylation. ... strand of this site in the presence of Dnmt1. Kinetically this reaction scheme means that two methyl groups are always introduced, one slowly by Dn...

Ngày tải lên: 21/02/2014, 01:21

... specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions Ronald P. de Vries 1† , Lucie Par ˇ enicova ´ 1‡ , Sandra W. A. Hinz 2 , ... Potato arabinogalactan consists of 86% D -galactose and 6.6% L -arabinose, while soy arabinogalactan consists of 57% D -galactose and 38% L -ar...

Ngày tải lên: 21/02/2014, 01:21

... Hunter et al. (Eur. J. Biochem. 269) Ó FEBS 2002 Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue The ´ re ` se ... Peplow, F. Yamakura and T. Matsumoto for the analyses of iron and manganese in protein samples. We also wish to thank H. Steinma...

Ngày tải lên: 21/02/2014, 01:21

... that of C. jejuni. In summary, we have presented the complete structures of the LOS and capsule polysaccharide of the genome sequenced strain C. jejuni NCTC 11168. The outer core LOS of NCTC 11168 ... The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC...

Ngày tải lên: 21/02/2014, 01:21

... aliquots of the reaction Ó FEBS 2002 A mammalian 14-kDa phosphohistidine phosphatase (Eur. J. Biochem. 269) 5017 Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase Pia ... observed a phosphatase activity that was insensitive towards okadaic acid and EDTA. This suggested the existence of a phosphohistidine phosphata...

Ngày tải lên: 21/02/2014, 01:21

... unfolding, will also be discussed. A new approach of image analysis, which is established by us recently [4,11], was used to analyze the unfolding dynamics and also was introduced into the analysis of the ... C., Narayan, M., Wedemeyer, W.J. & Scheraga, H .A. (2000) Acceleration of oxidative folding of bovine pancreatic ribonuclease A by anion-induced stabilization and...

Ngày tải lên: 21/02/2014, 01:21

... suggest that certain enzymes, and therefore the corresponding genes of the A2 0 1A biosyn- thetic pathway, may be related to their counterparts of the puromycin and hygromycin A biosynthetic pathways, respectively. The ... Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A2 0 1A from...

Ngày tải lên: 21/02/2014, 01:21

... Biochem. 269) 6061 Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum Surza L. G. Rocha 1 , Bruno Lomonte 3 , Ana G. C. Neves-Ferreira 1 , ... physicochemical and biological properties of DM64, an antimyotoxic protein from opossum serum. DM64 is an acidic protein showing 15% gly...

Ngày tải lên: 21/02/2014, 01:21

... the C(17) group of the ste roid. 1402 P. Auvray et al. (Eur. J. Biochem. 269) Ó FEBS 2002 Study of substrate specificity of human aromatase by site directed mutagenesis P. Auvray 1, * ,† , C. ... this case, by introducing a hydroxyl group, accessibility of the substrate to the active site is reduced, as observed with the mutants K119T and K11 9Y. By keeping a...

Ngày tải lên: 21/02/2014, 15:20

... Christian-Albrechts-University of Kiel, Germany To gain insight in the subcellular localization of tumor necrosis factor receptor-associated factor (TRAF4) we analyzed GFP chimeras of full-length TRAF4 and various deletion ... Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4...

Ngày tải lên: 21/02/2014, 15:20

... individual enzymes. However, the study of the enzymes of the tricarboxylic acid cycle necessarily also entails the s tudy of the several enzymes involved in t he glyoxylate cycle in plants, because three ... the evolution of the enzymes of the pyru vate dehydrogenase (PDH) complex, the tricarboxylic acid cycle, and the glyoxylate cycle...

Ngày tải lên: 22/02/2014, 04:20

... activity of the smaller form of the bifunctional e nzyme was far lower than that of the larger form of the enzyme [11]. This is reminiscent of the enzyme from rat liver in which partial proteolysis ... previously [1]. In conclusion, the kinetic properties of the recombinant enzyme are in a greement with t hose of t he enzyme isolated from spinach leaves. This sugges...

Ngày tải lên: 22/02/2014, 04:20

... 2002 Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase Konstantinos Mavromatis 1, *, Iason Tsigos 2, *, Maria Tzanodaskalaki 2 , Michael Kokkinidis 1,3 and Vassilis ... the active site of the psychrophilic alkaline phosphatase from the Antarctic Table 1. Enzymatic and thermodynamic parameters of the psychrophilic...

Ngày tải lên: 22/02/2014, 04:20

... many people are in your family? (8) What is your ethnicity? (9) What is your occupation (peasant, laborer, small private businessman or cadre official)? (10) How many years did you study? ... is your yearly income? (12) Have you been vaccinated for HBV (yes/no)? (13) Do you have a family history of HBV? Information on demographics and behavioral factors was obtained. The study ... HBs...

Ngày tải lên: 08/08/2014, 18:21