Báo cáo khoa học: " In vitro Douglas fir pollen germination: influence of hydration, sucrose and polyethylene" docx

... increase in the number of tubes was observed between dry and rehydrated pollen. Increasing sucrose and PEG concentrations caused a decline in the propor- tion of germinating ... 26], resulting in poor tube morphology or in partial or total inhibition of its growth. In both flowering plants and gymnosperms such as Douglas fir and...

Ngày tải lên: 08/08/2014, 14:21

... by using 32 P-labeling primer or template in the presence or absence of DNA-binding agents (DDIs and doxorubicin). The DNA bulge binding of both compounds was detected by CD and UV melting experiments. ... the ligand–nucleic acid complex is formed. To gain insight into the binding of drugs to bulge DNA, several bulge- containing oligonucleotides were selected as binding hosts for...

Ngày tải lên: 07/03/2014, 06:20

... hpf embryos were incubated during 15 min with different kinase inhibitors at 30 °C and then used to analyse in vitro recom- binant CNBP phosphorylation in kinase buffer containing 100 lm ATP and 5 lCi ... November 2006) doi:10.1111/j.1742-4658.2006.05596.x The zinc-finger cellular nucleic acid binding protein (CNBP) is a strikingly conserved single-stranded nucleic acid binding prote...

Ngày tải lên: 16/03/2014, 12:20

... key role of zinc [43–46] and copper [47,48] in Ab plaque formation. The finding that in vitro c -secretase cleavage of APP is inhibited by physiologically relevant concentrations of zinc places metal-mediated ... & Evin G (2004) Characterization of presenilin complexes from mouse and human brain using Blue Native gel electro- phoresis reveals high expression in embryoni...

Ngày tải lên: 16/03/2014, 23:20

... between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by meas- uring inhibition of the endonuclease reaction [36]. In the ... and its binding affinity is not altered by the presence of DNA in the DNA binding site. Interestingly, when 3 n M RNA and 10 n M DNA were added simultaneously to a limiting amount...

Ngày tải lên: 17/03/2014, 10:20

... pneumoniae SPase I For in vitro self-cleavage of S. pneumoniae SPase I in the presence of phospholipid, 20 lL of reaction containing 5 lg of purified SPase I was incubated in 37 °Cfor2hin 20m M Tris/HCl ... the protein. The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23]. In vitro self-cleavage was also observed in all investigated...

Ngày tải lên: 23/03/2014, 21:21

... dissec- tion of the folding mechanism of the a subunit of tryp- tophan synthase: an amino-terminal autonomous folding unit controls several rate-limiting steps in the folding of a single domain protein. ... display), increasing the likelihood of correct folding in a redu- cing environment while minimizing the risk of proteo- lytic degradation. The association of plasmid and...

Ngày tải lên: 30/03/2014, 20:20

... properties of PTOX in vitro. In this report, we produced the protein in E. coli and studied its substrate preferences, the factors in uencing its activity and the nature of suitable metal cofactors and ... This might explain their different substrate preferences. A fuller understanding of quinone–protein interactions in this class of enzyme may be achieved by testing t...

Ngày tải lên: 31/03/2014, 07:20

... spectra (in KBr) of lentinan samples and the immunologically active b-glucan from Saccharomyces cerevisiae. The arrows indicate absorption bands typical of lipids (a and b), and proteins (c and d). C. ... demonstrated direct inhibition of the proliferation of human breast cancer cells in vitro and immunostimulatory properties in terms of mitogenic and co-mitogenic...

Ngày tải lên: 28/06/2014, 11:20

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Ngày tải lên: 07/08/2014, 18:21