Báo cáo khoa học: " In vitro Douglas fir pollen germination: influence of hydration, sucrose and polyethylene" docx
Báo cáo khoa học: " In vitro Douglas fir pollen germination: influence of hydration, sucrose and polyethylene" docx
...
increase
in
the
number
of
tubes
was
observed
between
dry
and
rehydrated
pollen.
Increasing
sucrose
and
PEG
concentrations
caused
a
decline
in
the
propor-
tion
of
germinating ...
26],
resulting
in
poor
tube
morphology
or
in
partial
or
total
inhibition
of
its
growth.
In
both
flowering
plants
and
gymnosperms
such
as
Douglas
fir
and...
Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... by using
32
P-labeling primer or template
in the presence or absence of DNA-binding agents
(DDIs and doxorubicin). The DNA bulge binding
of both compounds was detected by CD and UV
melting experiments. ... the
ligand–nucleic acid complex is formed. To gain insight
into the binding of drugs to bulge DNA, several bulge-
containing oligonucleotides were selected as binding
hosts for...
Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... hpf
embryos were incubated during 15 min with different kinase
inhibitors at 30 °C and then used to analyse in vitro recom-
binant CNBP phosphorylation in kinase buffer containing
100 lm ATP and 5 lCi ... November
2006)
doi:10.1111/j.1742-4658.2006.05596.x
The zinc-finger cellular nucleic acid binding protein (CNBP) is a strikingly
conserved single-stranded nucleic acid binding prote...
Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... key role of zinc [43–46] and
copper [47,48] in Ab plaque formation. The finding
that in vitro c -secretase cleavage of APP is inhibited by
physiologically relevant concentrations of zinc places
metal-mediated ... & Evin
G (2004) Characterization of presenilin complexes from
mouse and human brain using Blue Native gel electro-
phoresis reveals high expression in embryoni...
Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... between inhibition of
endonuclease activity and inhibition of splicing activity
indicating that RNA binding can be monitored by meas-
uring inhibition of the endonuclease reaction [36].
In the ... and its
binding affinity is not altered by the presence of DNA in the
DNA binding site.
Interestingly, when 3 n
M
RNA and 10 n
M
DNA were
added simultaneously to a limiting amount...
Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
... pneumoniae
SPase I
For in vitro self-cleavage of S. pneumoniae SPase I in the
presence of phospholipid, 20 lL of reaction containing
5 lg of purified SPase I was incubated in 37 °Cfor2hin
20m
M
Tris/HCl ... the protein. The self-cleavage of LexA protein is
important in the SOS response in bacteria [21–23]. In vitro
self-cleavage was also observed in all investigated...
Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx
... dissec-
tion of the folding mechanism of the a subunit of tryp-
tophan synthase: an amino-terminal autonomous
folding unit controls several rate-limiting steps in the
folding of a single domain protein. ... display),
increasing the likelihood of correct folding in a redu-
cing environment while minimizing the risk of proteo-
lytic degradation. The association of plasmid and...
Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot
... properties
of PTOX in vitro. In this report, we produced the protein in
E. coli and studied its substrate preferences, the factors
in uencing its activity and the nature of suitable metal
cofactors and ... This might explain their
different substrate preferences. A fuller understanding of
quinone–protein interactions in this class of enzyme may be
achieved by testing t...
Báo cáo khoa học " In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes " pdf
... spectra (in KBr) of lentinan samples and the immunologically active b-glucan from Saccharomyces cerevisiae. The
arrows indicate absorption bands typical of lipids (a and b), and proteins (c and d).
C. ... demonstrated direct inhibition of the proliferation of human breast cancer cells in vitro and immunostimulatory
properties in terms of mitogenic and co-mitogenic...
