Báo cáo khoa học: "Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)" ppsx

... +82-31-467-1883 E-mail: kweonch@mail.nvrqs.go.kr Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30 T -) Seong In Lim, Wooseog ... 92.3% (Table 2). Overall, recombinant BLV gp51/gp30 T - was more sensitive than the traditional FLK Immunodiffusion analysis using baculovirus-expressed...

Ngày tải lên: 07/08/2014, 23:22

... Natural Language Processing, pages 495–498. Taku Kudo and Yuji Matsumoto. 2002. Japanese dependency analysis using cascaded chunking. In Proc. of the 6th Conference on Natural Language Learning 2002 (CoNLL ... Marcus. 1995. Text chunking using transformation-based learning. In Proc. of the third workshop on very large corpora, pages 82–94. Dan Shen and Mirella Lapata. 2007. Using se-...

Ngày tải lên: 20/02/2014, 04:20

... Cell numbers were counted using a Coulter counter (Multisizer 3, Beckman Coulter Inc., Fullerton, CA, USA), using a 30 lm aperture. Experimental time-dependent regulation analysis K. van Eunen et ... time-dependent regulation analysis K. van Eunen et al. 5530 FEBS Journal 276 (2009) 5521–5536 ª 2009 The Authors Journal compilation ª 2009 FEBS fundamentally different from other types o...

Ngày tải lên: 18/02/2014, 06:20

... explicitly using morphology-based fea- tures in our models improves the system’s perfor- mance. We also measure the impact of using a wide coverage polarity lexicon and show that using a tai- lored ... sentiment analysis. Computational Lin- guistics, 35(3):399–433. J. Yi, T. Nasukawa, R. Bunescu, and W. Niblack. 2003. Sentiment analyzer: Extracting sentiments about a given topic using...

Ngày tải lên: 20/02/2014, 05:20

... apoptosis-related proteins previously identified by others, using MS ⁄ MS analysis, such as RIP1, Smac, STAT3, B23, and CaM, were confirmed here by immunoblot analysis to be phosphorylation- dependent 14-3-3-associated ... 14-3-3-binding proteins by protein micro- array analysis. J Neurosci Methods 152, 278–288. 19 Puri P, Myers K, Kline D & Vijayaraghavan S (2008) Proteomic analysis...

Ngày tải lên: 06/03/2014, 22:21

... analysis of unknown serial analysis of gene expression tags; rSAGE, reverse serial analysis of gene expression; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis of unknown SAGE ... existing expressed sequences upon analysis of the SAGE data Keywords modified lock-docking oligo(dT); mRNA; RACE; serial analysis of gene expression (SAGE); two-step analysis of...

Ngày tải lên: 07/03/2014, 04:20

... verified using SDS/ PAGE. Protein concentrations were determined using the Bradford assay kit provided by Bio-Rad (Hercules, CA, USA). Enzyme assays The activity of ChiB variants was determined using ... performed using the QuikChange TM Site-directed Mutagenesis Kit from Strata- gene (La Jolla, CA, USA) essentially as described by the manufacturer. Sequences of mutated chiB fragments...

Ngày tải lên: 07/03/2014, 14:20

... were detected by immunoblot analysis with anti-IgG (Fig. 1C,D). The different migration behaviour reflects the different sizes of the fusion proteins. Immunological analysis revealed the presence ... KU1234 and KU617) and subcloned into pPC97 by using the primer-derived SalI ⁄ NotI sites (pIB1 ⁄ 26). The corresponding construct in pPC86 was designed using restriction sites SalI ⁄ SacI...

Ngày tải lên: 07/03/2014, 16:20

... (2005) 4114–4128 ª 2005 FEBS using LdUSP-RR2 (68 °C) and LdUSP-RF2 (68 °C), respectively. DNA sequencing and sequence analysis PCR products were purified by agarose gel electrophoresis and cloned ... mm )1 , Institute of Isotopes Co.), were sep- arated in 10% SDS ⁄ polyacrylamide gel and analyzed using BAS-2000. Gel mobility shift assay was conducted with in vitro translated LdEcR...

Ngày tải lên: 07/03/2014, 21:20

... 60607 USA bdieugen@cs.uic.edu Abstract We discuss Feature Latent Semantic Analysis (FLSA), an extension to Latent Semantic Analysis (LSA). LSA is a statistical method that is ordinar- ily trained ... dia- logues. 1 Introduction In this paper, we propose Feature Latent Semantic Analysis (FLSA) as an extension to Latent Seman- tic Analysis (LSA). LSA can be thought as repre- senting the...

Ngày tải lên: 08/03/2014, 04:22