Báo cáo khoa học: "Anti-obesity activity of diglyceride containing conjugated linoleic acid in C57BL/6J ob/ob mice" potx

... expressio n of PPAR-γ increased with the treatment of diglyceride (B), conjugated linoleic acid (C), and diglyceride containing 22% conjugated linoleic acid as fatty acid (D). acids in C57BL/6J ob/ob ... effect of CLA -containing diglyceride in obese mice 191 Fig. 1. Change in body weights of C57BL/6J ob/ob male mice for 5 weeks. DG: diglyceride,...

Ngày tải lên: 07/08/2014, 23:22

... pK a is consistent with the involvement of an acidic amino acid in the binding of excess BTC. Excess-substrate activation for human BuChE has been attributed to binding of positively charged substrates, ... excess-substrate binding appears to involve the intricate interplay of a variety of residues in the active site. pH dependence of A328W The A328W mutant has a remarkable...

Ngày tải lên: 20/02/2014, 23:20

... to investigate whether calpain 7 interacts with IST1 through MIT– MIM interactions. In this study, we examined cal- pain 7–IST1 interactions by in vitro and in vivo binding experiments, using ... MIM1 and MIM2 bind to only one MIT domain or bind to each of the two MIT domains of calpain 7, and it is not known why interaction between calpain 7 and the MIM2 -containing protein CHMP6...

Ngày tải lên: 18/02/2014, 04:20

... conformational transition upon binding to BchH may bring the integrin I domain of BchD into contact with the integrin-binding motif of BchH, simultaneously triggering porphyrin metallation. This would ... shown binding of Mg 2+ to this subunit [21]. An integrin I domain, suggested to exist in the C-terminus of the 70 kDa subunit, is a metal binding site (MIDAS motif) that can be exp...

Ngày tải lên: 19/02/2014, 12:20

... meso-phenyl rings being recognized by th e side chains of amino acids of the antibody [36]; (b) in the case of 13G10, one carboxylic acid residue of the protein could participate in the catalysis of the ... pyrrole rings packed against tyrosine residues of the V L domain. Binding of imidazoles to the iron(III) of Fe(ToCPP) and Fe(ToCPP))13G10 The second part of our res...

Ngày tải lên: 21/02/2014, 03:20

... N-terminal arm. The N-terminus of PsHsp18.1.C-I was also implicated in substrate interactions in bis-ANS binding experiments [15]. As it is proposed that substrate binding and protection involves ... of the chaperone. Using this assay we tested the relative activity of the two wheat proteins in suppressing aggregation of MDH. As shown in Fig. 5, both wheat sHsps were effecti...

Ngày tải lên: 07/03/2014, 15:20

... specific proteins in the hemolymph, namely LPS-binding proteins, b-glucan b inding proteins [2], or lectins [3]. b-1,3-Glucan binding proteins have been identified in Penaeus californiensis and in L. ... from the N-terminus domain of histone H2A: buforin I (39 amino acids), parasin I (21 amino acids), and hipposin (51 amino acids ) from the Asian toad stomach and catfish and halibut skin...

Ngày tải lên: 07/03/2014, 16:20

... blot using polyclonal antibody against purified adenylate kinase (Fig. 5B). Autophosphorylation activity The autophosphorylating activity of Ndk was determined by incubating purified protein with ... Ndk-associated GTPase activity was demonstrated in a filter-binding assay by incubating Ndk with [c- 32 P]GTP which resulted in hydrolysis of 60% bound GTP in 30 min (Fig. 7B). Third...

Ngày tải lên: 08/03/2014, 08:20

... (A) Native gels showing in situ enzymatic staining for ATP or ADP hydrolysis activity of the Q-fraction of NtrC1 C . Arrows indi- cate positions of the NtrC1 C and apyrase proteins located by Coo- massie ... contam- ination revealed a heterogeneity in the ATPase preparation, yielding two subfractions. The resulting, more homogeneous preparation of an NtrC1 C variant bearing a si...

Ngày tải lên: 16/03/2014, 04:20

... such a binding constant, by study- ing an alternative activity of the enzyme where the substrate of interest acts as an inhibitor (or competi- tive substrate, see Appendix). The high binding affinity of ... determination of the inhibition pattern of CH 2 H 4 folate, which is treated as a dead- end inhibitor for FDTS oxidase activity; and (b) examination and validation of the ass...

Ngày tải lên: 23/03/2014, 04:21