Báo cáo khoa học: "Genetic characterization of Barbari goats using microsatellite markers" docx

... Barbari goats using 21 microsatellite markers. Detailed Genetic characterization of Barbari goats 75 of alleles observed, alleles size, polymorphism information content and heterozygosity of Barbari ... the Barbari goats for further improvement of performance. Keywords: Barbari goats, genetic characterization, heterozygosity, microsatellite markers, polym...

Ngày tải lên: 07/08/2014, 23:22

... Mapping and characterization of the origin of DNA replication of circovirus. J. Virol. 1997, 71(3), 2562-2566. 32. Mankertz J., Buhk H J., Blaess G, and Mankertz A. Transcription analysis of porcine ... homology of Korean isolates, but PC201DJ had exact consensus ORF2 with AF118097. The functions of 104 amino acids of ORF3 and 59 amino acids of ORF4 have been not clearly rep...

Ngày tải lên: 07/08/2014, 15:20

... (A) Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identified in each step of the screen. (C) Distribution of the 63 distinct sequences in relation of their position ... 3¢ UTR. In recent years, and as a result of great efforts aiming to understand the mechanism of RNA control in animals, the function of a diverse number of RNA-binding proteins...

Ngày tải lên: 18/02/2014, 06:20

... in the course of the reaction. The principle of the assay is that the presence of a SOD inhibits the reduction of cytochrome c. This was vali- dated by adding a total of 2.5 U of commercial SOD ... kinetics and mechanisms of the reactions of iron(III) with b-dik- etones. The formation of monoacetylacetoatoiron(III) and the effect of copper (II) on the formation of monoth...

Ngày tải lên: 18/02/2014, 06:20

... 860 Phos- phorimager and quantified using imagequant software (Molecular Dynamics). Data were fit using the kaleida- graph curve-fitting program (Synergy Software) using the equation: h = [ribozyme] u ⁄ ... constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentration of unbound ribozyme in the reaction. Measurement of...

Ngày tải lên: 18/02/2014, 18:20

... splitting constants of the 15 N nucleus of the distal NO and of the 14 N nucleus of the proximal His of the GmHO-1 complex are closer to those of the rHO-1 complex than to those of the SynHO-1 complex. ... complex of GmHO-1 at pH 7.0 (0.1 M, KPB). a-1, Expansion of the g xy region of (A) (solid line) and of the corres- ponding part of the spectrum of ferric heme–rHO-...

Ngày tải lên: 19/02/2014, 05:20

... to induction of the structure of the N-domain. Measurements in the presence of the osmolyte, trimethylamine N-oxide, which induces the structure of unfolded proteins, show that formation of the coupled ... endothermic. The heats of dilution of the peptide and the buffer were small com- pared with the heat of reaction. Data were fitted using a nonlinear least squares routine...

Ngày tải lên: 19/02/2014, 05:20

... forms of AUH were pro- duced as fusions to the MBP of E. coli. The purification of the gene products was carried out as previously described [17]. Protein was estimated using the method of Bradford [24]. Production ... pre- pared by incubation of 3-methylcrotonyl-CoA with 3-methylcrotonyl-CoA-carboxylase in the presence of NaH 14 CO 3 . In this work, K m values for the hydration...

Ngày tải lên: 19/02/2014, 07:20

... appearance of the signals of both methyl groups and of the methine group of the enzymat- ically formed DMAPP (Fig. 4A). Concomitantly, the signals of IPP were progressively diminished. Using acetate as ... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. The substrate binding site of the type II enzyme remains veil...

Ngày tải lên: 19/02/2014, 13:20

... 1.2 molar ratio of Ca 2+ ⁄ CaM to PhK5 had been reached. The inset shows an expanded view of the indi- cated area of the spectrum. (Bottom) An overlay of spectra from the titration of Ca 2+ ⁄ CaM ... in the two EF hand motifs of each CaM domain. The pres- ence of a relatively large number of Ca 2+ ⁄ CaM ⁄ pep- tide complexes in the PDB opens up the possibility of using the...

Ngày tải lên: 19/02/2014, 17:20