Báo cáo khoa học: "Cytokine response in Balb/c mice infected with Francisella tularensis LVS and the Pohang isolate" docx

... treated with trypsin in the presence of 0.2% Triton X-100; in lanes 4 and 5, valinomycin (Val) (0.1 lg per mg of mitochondrial protein) was present during the import incubation. (B) Mean values in ... CREB was investigated by incubating freshly isolated, intact rat liver mitochon- dria with the protein synthesized in the Rabbit Reti- culocyte Lysate (RRL) system with [...

Ngày tải lên: 07/03/2014, 02:20

... targeting may occur. Finally, an outline of the future challenges in this field is provided, and the insights that may be gained from a greater under- standing of the mechanism of dual targeting ... proteins. Defining the plastidic and mitochon- drial receptor(s) for dual-targeted proteins would represent a major landmark, in that a comparison with the binding of location-sp...

Ngày tải lên: 07/03/2014, 03:20

... were compared in formalin fixed and paraffin embedded CHV -infected cells. The optimum result was obtained using digoxigenin-labeled probe, 100 µ g/ml proteinase K pre-treatment, and overnight hybridization. ... detecting canine herpesvirus (CHV) and compared for their sensitivities by in situ hybridization (ISH) in formalin fixed, paraffin embedded sections, which has been use...

Ngày tải lên: 07/08/2014, 17:22

... °C. The dialysates were used in the reference cell and for buffer baseline determination. The thermograms obtained were analyzed using the computer program cpcalc (Calorimetry Sciences Corp.), and ... ssDNA and plasmid were analyzed using linearized pBAD ⁄ gIII plasmid, linea- rized and denatured plasmid and intact plasmid. The pBAD ⁄ gIII plasmid was linearized using Sa...

Ngày tải lên: 19/02/2014, 05:20

... of Ngb and Mb. The denatur- ation of the proteins was monitored by recording the decrease in the ellipticity at 222 nm in the far-UV CD spectra of the protein in the presence of increasing concentrations ... being the endogenous ligand. Therefore, Ngb ligand binding requires the displacement of the intramolecular ligand His64 bound to the heme iron. Hexacoordinat...

Ngày tải lên: 07/03/2014, 02:20

... maximum in the near ultraviolet region of the UV-A spectrum with a long tail extending into the infrared region. The two other UV absorbing compounds contain one of two different ring units: an aminocyclohexenone ... photosynthesis in another dinoflagellate, Gymnodinium sanguineum [27]. It is evident from the present investigation that the studied cyanobacteria are able to in...

Ngày tải lên: 07/03/2014, 15:20

... caused by the loss of Ccm3 interaction with the kinases ser- ine ⁄ threonine kinase 25 (STK25) and mammalian ster- ile twenty-like 4 (MST4), giving a hint to the signaling pathway in which Ccm3 ... help translate the cellular and biochemical insights into a better understanding of disease mechanism. In this minireview, we discuss the contributions of animal models to our g...

Ngày tải lên: 15/03/2014, 10:20

... candidates. The pro- gram fithelices (Doc. S1) was used to determine the optimal splicing positions for joining the coiled coil fusion candidate to KcsA. The indicator used by the program is the ... ends at the ends of C-terminal tetramerization domains. The different structural domains are indicated by the bars above the protein sequence. The linker between the KcsA p...

Ngày tải lên: 16/03/2014, 00:20

... °C in the linear response range for 20 min (varying glycine) or 30 min (varying H-protein). Rates obtained without substrate were sub- tracted, and kinetic parameters were calculated by nonlin- ear ... metabolic environments. In this study, we examined whether the changes to the N-terminus of Flaveria H-protein alter its reactivity with P-protein, the actual glycine-decarbox...

Ngày tải lên: 23/03/2014, 04:20

... protein (dFXRP). The corresponding missense mutation in dFXRP, the I307N, has pro- nounced effects on the in vivo activity of the protein. The effect of the point mutation on the structure and ... the effect of the I307N mutation, which occurs in the second KH domain, we have cho- sen to use the tandem KH1-KH2 construct rather than the isolated KH2 domain, beca...

Ngày tải lên: 30/03/2014, 15:20