Báo cáo khoa học: " Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin- neuraminidase proteins" pdf

Báo cáo khoa học: Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells ppt

... demonstratethat antioxidants carried in chylomicron remnants enhancelipid accumulation in macrophages, and that this is causedby a markedly increased rate of uptake of the particles and by a raised ... (CHAOS). Lancet 347, 781–786.11. Clarke, R. & Armitage, J. (2002) Antioxidant vitamins and risk of cardiovascular disease. Review of large scale randomised trials.Cardiovasc. Drugs Ther. ... in the same system. Data are expressed as OD units and as the percentage of the values found with untreated (control) macrophages, and represent the mean ± SEM from three experiments. ABCA1,ATP-binding...

Báo cáo khoa học: Characterization of oligosaccharides from the chondroitin/dermatan sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides doc

... Oliveira FW, Chavante SF, Santos EA, Dietrich CP& Nader HB (1994) Appearance and fate of a beta-galactanase, alpha, beta-galactosidases, heparan sulfate and chondroitin sulfate degrading ... material for the study of intact CS chains.HexasaccharidesAlthough data from disaccharides, trisaccharides and tetrasaccharides are valuable for the detailed structuralcharacterizations of ... were of analytical grade.Isolation of CS from cartilage and DS from lungCS was isolated from fresh articular and tracheal carti-lages as previously described [4,5], and DS was isolatedfrom...

Báo cáo khoa học: Abundance of intrinsic disorder in SV-IV, a multifunctional androgen-dependent protein secreted from rat seminal vesicle pot

... sepa-ration between sets of natively unfolded and ideallyglobular proteins. Following the same rationale asabove, we compared the mean value of the artiﬁcialparameter for SV-IV and the two ... methodsProtein databases The database of disordered proteins was created using a list of natively unfolded proteins [39] and the SWISS-PROTprotein sequence data bank [64]. The ideal database of globular ... the globular state was calculated according to [35]. The meannet charge was deﬁned as the absolute value of the differ-ence between the numbers of positively and negativelycharged residues at pH...

Báo cáo khoa học: Regulation of output from the plant circadian clock pot

... 11819–11820.76 Takai N, Nakajima M, Oyama T, Kito R, Sugita C,Sugita M, Kondo T & Iwasaki H (2006) A KaiC-asso-ciating SasA–RpaA two-component regulatory systemas a major circadian timing mediator ... (Betula pubescens), Lap-land diapensia (Diapensia lapponica) and leatherleaf(Chamaedaphne calyculata), germination is controlledby day-length [25–28]. The existence of photoperiodiccontrol of ... C, Nater M, Apel K & Staiger D (1997)AtGRP7, a nuclear RNA-binding protein as a compo-nent of a circadian-regulated negative feedback loop inArabidopsis thaliana. Proc Natl Acad Sci USA...

Tài liệu Báo cáo khoa học: "Integration of Large-Scale Linguistic Resources in a Natural Language Understanding System" pdf

... semantic analysis, and pragmatic analysis. Each stage has been designed to use linguistic data such as the lexicon and grammar, which are maintained separately from the engine, and can easily ... into our natural language understanding system. Client- server architecture was used to make a large volume of lexical information and a large knowledge base available to the system at development ... information about the semantic structure of concepts associated with English words, particularly verbs. For example, the verb abridge has an associated case frame consisting of an agent...

Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx

... K, KagawaW, Takata M, Takeda S, Yokoyama S & Shibata T(2001) Homologous-pairing activity of the humanDNA-repair proteins Xrcc3.Rad51C. Proc Natl AcadSci USA 98, 5538–5543.20 Kagawa W, ... compilation ª 2010 FEBSExperimental proceduresDNA A 74-mer ssDNA (5¢-ACGGGTGGGGTGGACATTGACGAAGGCTTGGAAGACTTTCCGCCGGAGGAGGAGTTGCCGTTTTAATAAGGATC-3¢) (Hokkaido SystemScience, Hokkaido, Japan) and ... amino acids surround-ing Trp71 and Trp165 with Ala, and examined the binding activities of these mutants. The E16 6A and D16 7A mutants exhibited higher afﬁnities for bothssDNA and dsDNA than the...

Báo cáo khoa học: Purine nucleoside phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding pot

... h of incubation in the presence of SsCSC and PfCGC, the enzymatic activity of SsMTAPII and its mutant, expressed as a percentage of their control enzymes, reaches 86.8% and 51.8%, and 68% and ... in the presence of various oxidative folding catalysts (reactiva-tion assay). The catalytic activity of (A) SsM-TAPII and SsMTAPIIC259S ⁄ C261S and (B)PfPNP and PfPNPC254S ⁄ C256S was thenmeasured ... catalyticactivity measurements performed under standard condi-tions.Reactivation assay of SsMTAPII, PfPNP and theirCXC-lacking mutants The activity of SsCSC and PfCGC as catalysts of oxidativeprotein...

Báo cáo khoa học: Reaction of human UMP-CMP kinase with natural and analog substrates docx

... orL-3TCDP.ResultsAnalysis of the forward reaction catalysedby UMP-CMP kinase with natural substrates and nucleoside analogs The puriﬁed recombinant human UMP-CMP kinaseexpressed as a His-tag fusion was analyzed ... Figure 1A and Table 1 show the activity of human UMP-CMP kinase as a function of natural substrates, and the corresponding kinetic param-eters. The reaction rates were found to increase as a function of ... restrictedtoD-nucleotides at the donor site.Keywords: UMP-CMP kinase; antiviral analog; 3TC; AraC;phosphorylation.Nucleoside analogs constitute a familly of importantantiviral and anticancer drugs. Analogs of thymidine...

Báo cáo khoa học: Evaluation of potential regulatory elements identi®ed as DNase I hypersensitive sites in the CFTR gene doc

... prominent in Capan1pancreatic adenocarcinoma cells. Southern blots of DNase I digested (A) Capan1 and (B) Caco2 chromatin cleaved with BamHI and hybridized with the F34L prob e. In each panel, lanes ... cotransfection with pCMV/b. Each baris the average of at least four transfectionexperiments, with each sample assa yed intriplicate, and standard errors of t he mean areshown.Fig. 3. DNA atlas ... lines w ere analysed: the colon carcinomaCaco2 [6], the pancreatic adenocarcinomas Capan1 [6] and NP31 [7] and the Calu3 lung adenocarcinoma [8].Transient transfection assaysTransient expression...

Báo cáo khoa học: Regulation of DNp63a by tumor necrosis factor-a in epithelial homeostasis docx

... 5¢-TGACATGTTTTCTGACGGCAAC-3¢; Bax reverse, 5¢-GGAGGCTTGAGGAGTCTCACC-3¢;DNp6 3a forward, 5¢-GGAAAACAATGCCCAGACTC-3¢;DNp6 3a reverse, 5¢-GTGGAATACGTCCAGGTGGC-3¢;GAPDH forward, 5¢-GAAGGTGAAGGTCGGAGTC-3¢;GAPDH ... 5¢-AGGAGTCCGCATCTCCGTCAGTG-3¢; Noxa forward, 5¢-GAGATGCCTGGGAAGAAGG-3¢; Noxa reverse, 5¢-ACGTGCACCTCCTGAGAAAA-3¢; p21 forward, 5¢-AAGACCATGTGGACCTGT-3¢; p21 reverse, 5¢-GGTAGAAATCTGTCATGCTG-3¢; Bax forward, ... TNF -a and DNp6 3a . We have found that TNF -a destabilizesDNp6 3a by both proteasomal and caspase-dependentdegradation pathways. The degradation of DNp6 3a byTNF -a was attenuated by inhibition of...

Xem thêm