... lectin on SDS/PAGE gave a single band at apparent molecular mass of 34 kDa. The binding affinity of the lectin in the hemolymph of the freshwater crab, Paratelphusa jacquemontii, expressed O-acetyl ... showing a( 2–6) linkage. The sialic acid affinity of the Paratelphusa lectin was further proved by its inability to inhibit sialidase-treated BSM and bovine thyroglobul...
Ngày tải lên: 21/02/2014, 00:20
... dianthramide-derivatives, the carnation phyto- alexins. However, no data are available regarding the role of this enzymatic activity in the biosynthesis of methylated phenols other than the dianthramide-derivatives. ... isocratically, at a flow rate of 1mLÆmin )1 , and the volume of injected samples was 10 lL. The amounts of the residual initial phenolic substrate...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx
... L-glutamate. The activity of L-aspartate was adjusted to 100. b 30 mML-aspartate was used as amino donor for a- ketoglutarate, and 30 m ML-gluta- mate was used as amino donor for oxaloacetate. The activity ... respectively (Fig. 2C). In the paper chromatography analysis of amino acids (Fig. 3), the AATB3 also demonstrated the ability to transfer the a- amino of th...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: "The Safety and efficacy of a new self-expandable intratracheal nitinol stent for the tracheal collapse in dogs" ppt
... trachea to thoracic trachea. Fig. 1. Lateral radiographs before (a) and after (b) intratracheal placement of self expandable nitinol stent with flare ends in a dog with tracheal collapse. Collapsed ... located from the mid-cervical to the thoracic trachea increased the diameter of the entire cervical to thoracic tracheal area. Coughing and dyspnea disappeared...
Ngày tải lên: 07/08/2014, 20:23
Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... behavior is typical of integral membrane proteins. The polypeptide with an apparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1). Table 1. N-Terminal ... Materials and methods. (A) Data points correspondtotheamplitudeofthetroughcenteredatg ¼ 1.951; as in the low po tential range, the radical signal of the dyes overlap in t...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... of A DNA binding domain B Ligand binding domain Fig. 1. Sequence alignment. (A) Alignment of DNA binding domain (C domain) and its C-terminal extension. (B) Alignment of ligand binding domain ... binding of SmNR1(Ile247-Ser372) and SmRXR1(Glu251-Asn376) in vitro. DNA binding of a protein containing 20 amino acids at the 5¢ end of the DBD, the DBD and the 40 a...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Purification and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily doc
... 4). Activities of purified arginase and H. pylori arginase- containing extracts with divalent cations Mammalian arginases require manganese for optimal catalytic activity, although some of these arginases ... that RocF is neces- sary and sufficient for arginase activity, and that the enzyme has a number of interesting and unique features among the arginase superfamily, i...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Purification and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx
... least six classes of cytosolic GSTs in insects [2]. The majority of GSTs are in the Delta and Epsilon classes, and the remain- ing enzymes are in the Omega, Sigma, Theta and Zeta classes. The ... Corp., Carlsbad, CA, USA), in accordance with the manufacturer’s instructions. Removal of contaminating agents from the crude RNA extract was performed using a Qia...
Ngày tải lên: 23/03/2014, 09:21
Báo cáo khoa học: Identification and characterization of a collagen-induced platelet aggregation inhibitor, triplatin, from salivary glands of the assassin bug, Triatoma infestans ppt
... 2957 Identification and characterization of a collagen-induced platelet aggregation inhibitor, triplatin, from salivary glands of the assassin bug, Triatoma infestans Akihiro Morita 1 , Haruhiko Isawa 2 , ... a 2 b 1 and a IIb b 3 integrins and secretion of platelet agonists such as ADP and thromboxane A 2 , accelerating platelet aggregation and thrombus formation. Th...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo khoa học: Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor pptx
... explored the effects of combining activating and inactivating mutations of the TSHr within a single receptor on its constitutive activity towards G s -alpha and G q -alpha and its binding characteristics ... both for the cAMP and IP pathways. These data show that an inactive form of the TSHr which is trapped inside a cell after transfection is able to gain the me...
Ngày tải lên: 08/03/2014, 08:20