Báo cáo khoa học: " Sequence analysis of the S1 glycoprotein

Báo cáo khoa học: " Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea" docx

... using one of the forward primers S1OLIGO5' (5'TGA AAACTGAACAAAAGA3') or NEWS1OLIGO5' (5'TG AAAACTGAACAAAAGAC3') together with one of the reverse primers S1OLIGO3' ... S1OLIGO3' (CTAAACTAACATAAGGG C3') or degenerate3'-2 (5'CCATAAGTAACATAAGGG CAA3') [14,16]. The RT reaction for synthesis of cDNA consisted of purifi...

Ngày tải lên : 07/08/2014, 20:23

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Báo cáo khoa học: Proteome analysis at the level of subcellular structures Mathias Dreger pot

... mass spectrometry along with the availability of comprehensive protein and DNA databases that made easy and quick protein identiﬁcation feasible. The analytical tools that are available nowadays allow the ... proteins in Golgi preparations from rat mammary gland cells in the state of maximal secretion at lactation as compared to that in a state of basal secretion. This u...

Ngày tải lên : 23/03/2014, 20:22

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Báo cáo khoa học: " Sequence Analysis of Canine LINE-1 Elements and p53 Gene in Canine Transmissible Venereal Tumor" doc

... pBLCAT2 vector. CAAGGCTCTCATTCAAAATGGAAGGAGAGATAAAGAGCTTCCAAGACAGGCAGGAACTGAAAGAATATGTGACCTCCAAA 80 CCAGCTCTGCAAGAAATTTTAAGGGGGACTCTTAAAATTCCCCTTTAAGAAGAAGTTCAGTGGAACAGTCCACAAAAACA 160 M M T L N ... 288 GTCAACCAGGAAATTAAGGAAGAATTAAAAAGATTCATGGAAACTAATGAGAATGAAGATACAACCGTTCAAAATCTTTG 1040 D A A K A V L R G K Y I A I Q A S I Q K L E R T Q I 314 GGATGCAGCAAAAGCAGTCCTGAGGGGGAAATACATCGCAAT...

Ngày tải lên : 07/08/2014, 15:20

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Báo cáo khoa hoc:"Comparative analysis on the structural features of the 5 ﬂanking region of κ-casein genes from six different species" pot

... AAC TTCTAAGAA ATA −1014: CTA TTCTGAGAA ATA −931: TGG TTCCCAGAA ACA −949: TCA TTCCAAGAA ACA PMF[13] ATCAN{0,8}TGAT −679: TAA ATCAGAATGAT CTG −726: GTG TGATCTAAATCA CAA TGATN{0,8}ATCA −597: AAG ... TTGAGGAAT ACA Rev: −298: TAT TTTAGCAAT AAC −1781: AAC CTTACCGAA GGA −214: ATT TTTAGAAAG CAC −1592: AAC ATTTCCCAA CAA Rev: −1577: AAC ATTTCCTCA TTT −481: TAA CTTACAAAACGC −639: TAT ATTACTGAA TTT −263:...

Ngày tải lên : 09/08/2014, 18:21

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Báo cáo khoa học: New insights into the P-glycoprotein-mediated efﬂuxes of rhodamines doc

... P-gp-associated efflux of rhodamine was calculated at the steady-state, taking into account the following points: (a) the diffusion (in ux and efflux) of rhodamine through the membrane is passive, ... mechanism. In this paper, we present data that further characterize the transport of several rhodamine analogues. We took advantage of the intrinsic fluorescence of rhodamin...

Ngày tải lên : 08/03/2014, 08:20

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Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence speciﬁc DNA binding proteins pptx

... however, yielded a major termination downstream of AAUAAA signal at the end of CAA*, A being the terminal nucleotide 16 295 of the mouse mt genome (189 nt transcript; Fig. 7A and C, lane 4). An additional major ... addition to AAUAAA sequence, in the termination process. Quantitation of the gel by radiometric imaging showed that nearly 60% of the RNA chains were...

Ngày tải lên : 08/03/2014, 08:20

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Báo cáo khoa học: Cholesterol interaction with the related steroidogenic acute regulatory lipid-transfer (START) domains of StAR (STARD1) and MLN64 (STARD3) ppt

... material The following supplementary material is available online: Fig. S1. Cholesterol binding of the START proteins. Fig. S2. Stability of the START domains of human STARD1 and STARD3 in the ... 35000 Intensity Intensity 0 5 10 15 20 0 10 20 30 40 50 29162.8+H 26167.8+H STARD1-START STARD3-START Fig. 1. Expression of the START domains of STARD1 and STARD3. (A) Domain...

Ngày tải lên : 30/03/2014, 04:20

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Báo cáo khoa học: S -Stereoselective piperazine-2-tert-butylcarboxamide hydrolase from Pseudomonas azotoformans IAM 1603 is a novel L-amino acid amidase doc

... 5¢-CGATCC AAGCTTTAAGGAGG AAtagGAAATGGAATTCATCGAAAAAATCCG-3¢ antisense primer, 5¢-TGCATCCA TCTAGAGCATTCA GC-3¢. The ampliﬁed PCR product was digested with HindIII and XbaI, separated by agarose ... codon ATG, and there was a palindromic sequence suggesting a termination structure downstream from the TGA stop codon of the gene. In the region of DNA upstream of the laaA...

Ngày tải lên : 23/03/2014, 12:20

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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... superposition of the exponential rise to analyze quantitatively the contribution of each phase. The amplitude and rate constant of each of the three phases can be calculated by deconvolution of the traces ... the J step was lowered and the subsequent rise was retarded. Kinetic information on each phase can be of great help in investigating the bioenergetics of...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... evaluating the in uence of Table 2. Thermodynamic parameters of the thermal denaturaturation of the diﬀerent onconase (ONC) variants at 25 °C and pH 2.0. (C Gdm/HCl ) ½ 13 , midpoint of guanidinium ... M., Nitta, K., Kawauchi, H., Takayanagi, Y. & Oyama, F. (1991) Comparative base speciﬁcity, stability, and lectin activity of two lectins from eggs of Rana catesbeiana an...

Ngày tải lên : 19/02/2014, 12:20

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