... to investigate whether the cysteine proteinase legumain is involved in the activation. The presence of this proteinase was shown in both cell lines, but incu- bation on monomeric 2D collagen did ... Germany). Production of conditioned media for MMP and TIMP determination To determine the secretion of MMP-2 and TIMP-1 into the media, trypsinized cells were suspended in serum-c...
Ngày tải lên: 18/02/2014, 11:20
... 6A,B). The protein content in the 8K-BLP-treated disks increased significantly, and was A E B C D Fig. 3. Determination of the structure of 8K-BLP. (A) MALDI-TOF MS analysis of the purified peptide. ... Members of the insulin-like peptide (ILP) family are present in a wide variety of metazoans. In vertebrates, insulin and insulin-like growth factors (IGFs) regulate...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc
... arginyl-tRNA synthetases as revealed by an examination of natural specificity variants Gabor L. Igloi and Elfriede Schiefermayr Institute of Biology, University of Freiburg, Germany The accuracy of protein ... between arginine and canavanine for aminoacylation of the plant tRNA transcript was observed when using [ 14 C]-canava- nine. At 0.4 mm canavanine, less tha...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx
... Journal 273 (2006) 257270 ê 2005 FEBS 261 A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site Diana Herna ´ ndez-Romero 1 , ... repor- ted the existence and expression of a multipotent lac- case and a tyrosinase in Marinomonas mediterranea [11,13...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt
... were of analytical grade from Nacalai Tesque (Kyoto, Japan) and Wako Pure Chemical Industries (Osaka, Japan). Culture and screening of bacteria Bacterial strains were cultivated for 15–20 h at ... & Komagata K (1992) Transfer of Pseudomonas- Amino- vorans (Dendooren Dejong 1926) to Aminobacter General-Nov as Aminobacter-Aminovorans Comb-Nov and description of Aminobacter-Aganoe...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: "Mining User Reviews: from Specification to Summarization Xinfan Meng Key Laboratory of Computational Linguistics " doc
... AFNLP Mining User Reviews: from Specification to Summarization Xinfan Meng Key Laboratory of Computational Linguistics (Peking University) Ministry of Education, China mxf@pku.edu.cn Houfeng Wang Key Laboratory ... Wang Key Laboratory of Computational Linguistics (Peking University) Ministry of Education, China wanghf@pku.edu.cn Abstract This paper p...
Ngày tải lên: 20/02/2014, 09:20
Báo cáo khoa học: Plasminogen activator inhibitor type-1 inhibits insulin signaling by competing with avb3 integrin for vitronectin binding pptx
... 1C), suggesting that PAI-1 might be competing with avb3 for VN binding. Moreover, we observed that urokinase- type plasminogen activator (uPA), which prevents PAI-1/ VN complex formation [22], was able ... cellular response to insulin. We demonstrate for the first time that PAI-1 modulates insulin signaling by preventing the binding of VN to avb3. This results in a dec...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: A chloroplast RNA binding protein from stromal thylakoid membranes specifically binds to the 5¢ untranslated region of the psbA mRNA potx
... to the AUG): T7-psbB5Â (5Â-GTAATACGACTCACTATAGGGTAAATTAATT TAATTTAAAATC-3Â)andpsbB3Â (5Â-TACACGATA CCAAGGTAAACC-3Â). Each template contained the pro- motor of the T7 RNA polymerase fused to the ... +13 relative to the AUG); T7 -psbA5 Â (5Â-GTAATACGACTCA CTATAGGGTACCATGCTTTTAATAGAAG-3Â)and 2054 (5Â-GATCCATGGTCATATGTTAATTTTTTTAA AG-3Â); )36 -RNA (wild-type sequence of th...
Ngày tải lên: 17/03/2014, 11:20
Báo cáo khoa học: Tec family kinases Itk and Rlk⁄ Txk in T lymphocytes: cross-regulation of cytokine production and T-cell fates pot
... associated with increased in ammation and Th1 cytokine production [4]. These results suggest that Tec kinases contribute to human diseases involving distinct types of T- cell activation and cytokine ... of distinct innate-type cytokine- producing T- cell populations in the thymus. Together, these results suggest that the Tec family kinases play critical roles in...
Ngày tải lên: 28/03/2014, 22:21
Báo cáo khoa học: "Immunolocalization of anion exchanger 1 (Band 3) in the renal collecting duct of the common marmoset" pptx
... Cl /HCO 3 exchanger in the monkey kidney is in a more active state in the collecting duct than in the CNT. Key words: band 3 protein, kidney, marmoset, monkey Introduction The collecting duct of the ... which were located in the Golgi region. In addition, there was no labeling of band 3 in the mitochondria of the CNT, CCD and in OMCD c...
Ngày tải lên: 07/08/2014, 20:23
báo cáo khoa học: "Double primary malignancies associated with colon Cancer in patients with Situs Inversus Totalis: Two Case Reports" pptx
... low anterior resection (black arrows). Double primary malignancies associated with colon Cancer in patients with Situs Inversus Totalis: Two Case Reports Young Wan Kim 1 , Hoon Ryu 1 , Dae ... versions will be made available soon. Double primary malignancies associated with colon Cancer in patients with Situs Inversus Totalis: Two C...
Ngày tải lên: 09/08/2014, 02:21
Báo cáo khoa học: "124I-HuCC49deltaCH2 for TAG-72 antigen-directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results" potx
... TAG-72 antigen- directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results. World Journal of Surgical Oncology 2010 ... on microPET imaging isexplainablebyouruseofchelatedformofthe Figure 5 Intravenous (i.v.) administration through the t ail vein of 7.4 MBq of 18 F-FDG for...
Ngày tải lên: 09/08/2014, 03:21
Báo cáo khoa học: "Intra-operative intra-peritoneal chemotherapy with cisplatin in patients with peritoneal carcinomatosis of ovarian cancer" pptx
... B, Kantelip JP, Pivot X: Serum and intraperitoneal pharmacokinetics of cisplatin within intraoperative intraperitoneal chemotherapy: influ- ence of protein binding. Anticancer Drugs 2005, 16:1009-1016. 21. ... 1 of 7 (page number not for citation purposes) World Journal of Surgical Oncology Open Access Technical innovations Intra-operative intra -peritoneal chemotherapy with...
Ngày tải lên: 09/08/2014, 04:21
báo cáo khoa học: "Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection" docx
... this article as: Lang et al.: Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection. BMC Plant Biology ... third of the strains exhibited more than 5% ARA with extraordinarily high values of 41.3% and 34.3% in Rhabdo monas incurva SAG 1271-8...
Ngày tải lên: 11/08/2014, 11:21
Báo cáo y học: " Mechanical ventilation modulates TLR4 and IRAK-3 in a non-infectious, ventilator-induced lung injury model" pptx
... Ventila- tory strategy also modulates alveolar and plasma levels of pro-inflammatory cytokines in patients with acute lung injury [5,31]. The ability of MV to induce inflammation may be in part ... Mantecón AG, Story ME, Pinto FM: Tachykinins and tachykinin receptors in human uterus. Br J Pharmacol 2003, 139:523-532. 20. Nakayama K, Okugawa S, Yanagimoto S, Kitazawa T, Tsukada...
Ngày tải lên: 12/08/2014, 11:20