Báo cáo khoa học: "In vitro susceptibilities of Leptospira spp and Borrelia burgdorferi isolates to amoxicillin, tilmicosin, and enrofloxacin" potx

Ngày tải lên: 07/08/2014, 18:21

... spectroscopy of DDI-1A (Fig. 4) showed a positive Cotton effect at 246 and 310 nm, and a neg- ative Cotton effect at 220 and 290 nm, whereas the CD of DDI-1B was almost complementary to that of DDI-1A. ... than those of their complementary strands, ATT and GT (Fig. 1B,C). As such, the enhancement effects of AB CDE F Fig. 1. Expansion of the various repeats and the effe...

Ngày tải lên: 07/03/2014, 06:20

... due to trapping of the DNA substrate by the RNA, because increasing the DNA concentration from 1 to 6 n M (with 3n M RNA) did not lead to any DNA cleavage. Tight stoichiometric binding of RNA to ... cleavage of the DNA substrate in the absence of RNA (see also Fig. 2D) and that the addition of competitor RNA to an endonuclease reaction masked the 1.025 kb DNA cleavage pr...

Ngày tải lên: 17/03/2014, 10:20

... protein in E. coli and studied its substrate preferences, the factors influencing its activity and the nature of suitable metal cofactors and inhibitors in vitro. Materials and methods Chemicals The ... inability of PTOX to use ubiquinone is an artefact of this assay (which relies on the ability of the membranes to reduce quinones). Therefore, we propose that the preferen...

Ngày tải lên: 31/03/2014, 07:20

... susceptibilities of the Brucella isolates identified in the Laboratory of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical medicine of the University of Crete. Methods A number of 74 strains ... Orran- tia-Gradin R, Hernandez-Oliva GM, Guttierrez-Rubio AT, Cardenosa O: Susceptibility of Mexican brucella isolates to moxi- floxacin, ciprofloxacin and other...

Ngày tải lên: 08/08/2014, 19:20

... Two clones (CI and GI) were test- ed with various carbohydrate sources: sucrose, glucose, fructose, galactose, mannitol and sorbitol. Carbohydrates were added to complete WPM ... (1.0 !M) and agar. The most extensive growth and num- bers of roots and leaves (Table I) were observed in media containing either glu- cose, galactose or fructose....

Ngày tải lên: 09/08/2014, 02:21

... keV/ μm of iron beam. Sensitivity to Genotoxic Chemical Agents Figure 6 shows the survival curves of the four chemical agents. Although camptothecin, mitomycin C and Figure 1 Characters of cell ... for only 1 to 4% of all primary malig nant bone tumors [1]. Chordoma originates from notochordal remnants and has slower local growth and metastasizes less frequently than other b...

Ngày tải lên: 09/08/2014, 09:21

... 1·. Gels were dried and autoradiography visualized by using GP-Storage Phosphor Screen and storm scanner and software (Amersham Biosciences). Radioactive bands were quantified and annealing percentage ... composed of unpaired strands, CNBP, hnRNP K, and additional factors [7]. Modulation of the concentrations and ⁄ or the biochemical activities of either CNBP or hnRNP K could...

Ngày tải lên: 16/03/2014, 12:20

... applied to the column, separated at a flow rate of 0.1 mLÆmin )1 , and fractions collected. Quantitation of c-secretase activity and inhibition by zinc nih image software (version 1.63) was used to ... c-secretase activity and cau- ses a subset of both Nct isoforms and PS2 to migrate in lower molecular mass fractions. Dimethylsulfoxide can modulate detection of COS7-SPC99...

Ngày tải lên: 16/03/2014, 23:20

... was puri- fied and cloned into the NdeIandXhoI sites of vector pET23b, resulting in pET23b-spiD1-36. The identity of the cloned gene was confirmed by DNA sequencing. For expression of SPase37–204, ... mL of lysis buffer with 15 m M imidazole. The protein was finally eluted out with 10 mL of elution buffer, and 1 mL of fractions were collected and analyzed by SDS/PAGE. In vitro...

Ngày tải lên: 23/03/2014, 21:21