Báo cáo khoa học: "Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf)" ppt

Ngày tải lên: 07/08/2014, 18:21

... numbers of mRNAs of L-chain and H-chains of the recombi- nant IgG, and sericin-1 per 10 ng of total RNA. The indicated values are 10 )5 of the actual copy numbers. b Data are the mean ± SEM of the ... that the absence of the core fucosylation is not a result of the absence of the fucosyltransferase expres- sion. The shortage of GDP-fucose in the cells might lead to the pre...

Ngày tải lên: 23/03/2014, 04:20

... inhibition of E. coli in plate assay using 100 pmol of each peptide, complete disappear- ance of ferroportin signal at the membrane of iron treated macrophage J774 cells in the presence of 700 l M of ... [27]. In our condi- tions, 10 lLofa10lm solution for either of the four peptides routinely resulted in a 10 mm diameter growth inhibition of E. coli whereas 10 lL of buffer...

Ngày tải lên: 18/02/2014, 18:20

... Fig. 1). Often, this kind of cleavage is followed by cyclization of the N-terminal glutamine to form pyroglutamic acid. The tryptic peptide mass fingerprint of TYR2 contained a peptide mass of 2136.108 ... 2136.196 Da). The identity of this peptide was then confirmed by MALDI-TOF ⁄ TOF fragment ion analysis, where partial sequences of this peptide were obtained from the ladders of...

Ngày tải lên: 19/02/2014, 06:20

... role of peroxidase in the early stages of melanogenesis, despite reports of the involvement of peroxidase–H 2 O 2 systems in later stages during the oxidation of indolequinone precursors of eumelanin and ... melanogenesis- inhibiting effects of chemical agents. Dermatology 205, 329–339. 6 Takahama U (1997) Enhancement of the peroxidase- dependent oxidation of dopa by compone...

Ngày tải lên: 07/03/2014, 17:20

... The activity of Pf-TDH was significantly increased by the addition of 2mm CoCl 2 (relative activity to that of the standard reaction 170%) and not by the addition of 2 mm ZnCl 2 or one of the other ... characterization of one of the selected enzymes, a novel l-threonine dehydrogenase, Pf-TDH (PF0991). The P. furiosus tdh gene encodes a protein of 348 amino acids and a calculate...

Ngày tải lên: 16/03/2014, 14:20

... with an anti-Aspf1polyclonal antiserum (Fig. 2B). The amino acid compositions of A C B Fig. 1. Structure of ribotoxins. Diagrams corresponding to the 3D structures of (A) a-sarcin and (B) a-sarcin ... Aspf1-specific IgE antibodies were detected in 26 out of 58 (44.8%) sera (six from the asthma and 10 from each cystic fibrosis and ABPA patients groups). The prevalence of sera having...

Ngày tải lên: 16/03/2014, 19:20

... the ampli- fication of two bands corresponding two the VH + CH1 + hinge + part of CH2 gene fragment of traditional antibodies or the VHH + hinge + part of CH2 gene fragment of HcAbs. Using the ... isolation of single- domain antibodies (sdAbs) consisting of the variable domain of these heavy chain antibodies, also named VHH [2]. These minimal antibody domains are endowed w...

Ngày tải lên: 23/03/2014, 05:22

... effect of osmolarity of the culture medium on in vitro development of interspecies somatic cell nuclear transfer whale embryos Type of embryo* Osmolarity of culture medium (mOsm/kg) † No. of embryos cultured No. ... pulses of 170 V/mm (25 μsec each) were used in our subsequent experiments. In the second Production of cloned sei whale embryos 289 Tabl e 4 . The effect of...

Ngày tải lên: 07/08/2014, 23:22

... classical hypothesis of a retention of the second polar body, a high level of meiotic postreduction may be involved at that locus. This is in good agreement with the results of T HORGAARD et ... produce, could be sterile and conse- quently of a great interest in aquaculture, where fish reproduction often makes the profi- tability decrease...

Ngày tải lên: 09/08/2014, 22:23